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![[Figure 3]](wuz5001fig3mag.jpg)
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Figure 3
Enhanced phase contrast in CPD-treated tissue imaged via propagation-based XPCT at P14. (a)/(d) Reconstructed tomographic slices from embedded (Epon) and CPD-treated tissue pillars, respectively, centred on the external plexiform layer (EPL) of the olfactory bulb, and parts of the glomerular layer (GL) and granule cell layer (GLC) are also visible. (b)/(e) Magnified views within the EPL. (c)/(f) Showing nucleolus features (yellow line: profile axis), with corresponding corrected phase-shift profiles in parts (g) and (h). (i) Overlay of line profiles from multiple samples containing the nucleolus feature {Epon: blue, n = [25, 25, 25] nucleoli; CPD: red, n = [25, 25, 25, 25] nucleoli)}, demonstrating consistent enhancement of phase-shift [corrected phase-shift = raw-signal − baseline mean (background)] in CPD samples. Shaded areas indicate standard deviation across measured regions. Reconstructed tomographic slices of embedded and CPD datasets labelled here are shown in Figs. S6 and S7, respectively. Other individual line profiles from each dataset are shown in Fig. S8. Increased phase contrast in CPD tissue results from the higher refractive index mismatch between the tissue and the surrounding air/vacuum, compared to resin-embedded preparations. |
![[Figure 3]](wuz5001fig3.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
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