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Figure 4
Enhanced phase contrast in CPD neuronal tissue imaged via XNH at ID16A. (a)/(d) Reconstructed slices from embedded (Epon) and CPD tissue, respectively, imaged in the external plexiform layer (EPL) and glomerular layer (GL) of the mouse olfactory bulb. (b)/(e) Magnified views within the EPL. (c)/(f) Showing nucleolus features (yellow line: profile axis), with corresponding corrected phase-shift profiles in parts (g) and (h). (i) Averaged line profiles from multiple nucleoli in Epon (blue, n = 100 nucleoli) and CPD (red, n = [100, 50] nucleoli) samples, illustrating enhanced phase modulation [corrected phase-shift  = raw-signal − baseline mean (background)] and gradient sharpness in dried tissue. Shaded bands represent standard deviation. Individual line profiles from each dataset is shown in Fig. S10.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 33| Part 3| May 2026| Pages 759-768
https://doi.org/10.1107/S1600577526001402
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