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Figure 5
Summary of phase-shift enhancement across all datasets and imaging modalities. Amplitude of the corrected phase-shift (rad/voxel), extracted from averaged line profiles across nucleolar features in CPD-prepared (red) and Epon-embedded (blue) neuronal tissue. Data points include measurements acquired at P14 (squares) and ID16A (circles). Special cases (measured at ID16A) are marked: star indicates a different brain region (thalamus) and smaller pillar diameter (150 µm), as shown in Fig. S11; diamond indicates a dataset collected under different imaging conditions, as detailed in Fig. S12. Despite differences in voxel size and imaging geometry, CPD consistently yields stronger phase-shift signals, confirming the generality of phase contrast enhancement introduced by CPD. Horizontal black bars indicate the mean value for each group.

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ISSN: 1600-5775
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