Synchrotron-based multi-scale biological imaging inside biocontainment environments

Cardoso, M.B.; Rabelo, R.S.; Polo, C.C.; Sanfelici, L.; Geraldes, R.R.; Moreno, G.B.Z.L.; Ramos, H.P.P.; dos Santos, G.R.; Pinto, A.C.; Lordano Luiz, S.A.; Westfahl, H.

doi:10.1107/S1600577526007083

Figure 2
(a) Integrated overview of the SIBIPIRUNA beamline, showing photon delivery and the two experimental stations located at SIRIUS and Orion, including their respective sample environments and transfer systems. (b) Optical layout based on dipole radiation, plane grating monochromator, and reflective optics for beam conditioning and energy selection in the soft X-ray regime. (c) Full-field transmission X-ray microscope configuration, highlighting the condenser optics, sample stage, Fresnel zone plate objective, and detector system used for cryogenic imaging. (d) Sample preparation and correlative workflow, illustrating cryogenic preparation, transfer, and integration with complementary imaging modalities.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 33| Part 5| September 2026|

https://doi.org/10.1107/S1600577526007083

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