Synchrotron-based multi-scale biological imaging inside biocontainment environments

Cardoso, M.B.; Rabelo, R.S.; Polo, C.C.; Sanfelici, L.; Geraldes, R.R.; Moreno, G.B.Z.L.; Ramos, H.P.P.; dos Santos, G.R.; Pinto, A.C.; Lordano Luiz, S.A.; Westfahl, H.

doi:10.1107/S1600577526007083

Figure 3
(a) Integrated overview of the TIMBÓ beamline, showing photon delivery, beam conditioning optics, and the two experimental branches, including the BSL-2 arthropod station and the high-containment (BSL-4) tomography station. (b) Optical layout based on a cryogenic permanent magnet undulator, double-crystal monochromator, and achromatic mirror system for high-coherence beam delivery over extended propagation distances. (c) Optical components in the vicinity of the sample position, highlighting the configuration used to shape and deliver the beam for coherence-based imaging modalities. (d) Sample preparation strategies for cryogenic imaging, illustrating alternative vitrification methods based on sample size, including high-pressure freezing (HPF) and controlled-rate freezing (CRF).

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 33| Part 5| September 2026|

https://doi.org/10.1107/S1600577526007083

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