Synchrotron-based multi-scale biological imaging inside biocontainment environments

Cardoso, M.B.; Rabelo, R.S.; Polo, C.C.; Sanfelici, L.; Geraldes, R.R.; Moreno, G.B.Z.L.; Ramos, H.P.P.; dos Santos, G.R.; Pinto, A.C.; Lordano Luiz, S.A.; Westfahl, H.

doi:10.1107/S1600577526007083

Figure 4
(a) Schematic overview of the HIBISCO beamline, showing photon delivery from the source to the in vivo experimental stations and the integration with BSL-2 and BSL-4 environments. (b) Optical layout, including the monochromator and focusing optics used to condition the high-energy X-ray beam for propagation-based phase-contrast imaging. (c) In vivo experimental platform, highlighting the animal positioning system, physiological monitoring, and motion-control components required for stable tomography. (d) Tomography cabins for BSL-2 and BSL-4 operation, implemented as sealed biocontainment enclosures compatible with animal handling, imaging requirements, and decontamination protocols.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 33| Part 5| September 2026|

https://doi.org/10.1107/S1600577526007083

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