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Figure 5
Correlative fluorescence and fast scanning SAXS analysis of sarcomeric organization in hiPSC-ACTN2-citrine-derived cardiomyocytes. (a) Inverted fluorescence image of α-actinin-labeled Z-disks with the periodic sarcomeric arrangement within the cardiomyocytes. The region within the black box highlights a representative area containing Z-disks. (b) The corresponding X-ray dark-field image reveals contrast differences between nucleus and the cytoplasm. (c) Orientation map derived from the fluorescence image of α-actinin-labeled Z-disks (d) SAXS-derived orientation map. The white outlines indicate the cells as shown in panel (a). (e) Overlay of the X-ray dark-field image with black lines representing the SAXS-determined orientation of the region within the black box in panel (a). Pixels within the white contour correspond to areas of high X-ray scattering intensity, where prominent Z-disks are observed in the fluorescence image. (f) Orientation histograms obtained from SAXS and fluorescence analysis for the same region within the white outline as shown in panel (e).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 33| Part 6| November 2026|
https://doi.org/10.1107/S1600577526009513