Structure of the Calcium-Binding Echidna Milk Lysozyme at 1.9 Å Resolution

The structure of the lysozyme isolated from the milk of the echidna, an Australian native monotreme, has been refined with data to 1.9 Å resolution. The nature of the calcium binding site is revealed.

Crystallographic Study of Yeast Copper Amine Oxidase

Orthorhombic crystals from the copper-containing amine oxidase from Hansenula polymorpha contain three dimers of 158 kDa molecular weight per asymmetric unit. A 12-site potassium mercuric iodide derivative was solved by molecular replacement and is being used to determine the structure of the protein by single isomorphous replacement combined with sixfold averaging and solvent flattening.

Bias Reduction in Phase Refinement by Modified Interference Functions: Introducing the γ Correction

The γ correction removes residual bias from refined electron density resulting in model structure factors that are each individually independent from their corresponding observed structure-factor amplitudes. This allows straightforward recombination of the two sources of information.

Accuracy of Determination of Position and Width of Molecular Groups in Biological and Lipid Membranes via Neutron Diffraction

This paper deals with the question of statistical accuracy of the position and width of molecular groups in membranes, determined in a neutron diffraction experiment.

Enhanced Diffractivity of Phosphoglucomutase Crystals. Use of an Alternative Cryocrystallographic Procedure

A protocol is described for transferring crystals of phosphoglucomutase, which are quite fragile, from 55% ammonium sulfate to 55% polyethylene glycol. The increase in diffractivity observed at 253 K is described and a possible rationale for this increase is considered.

Structure of Rabbit Muscle Phosphoglucomutase Refined at 2.4 Å Resolution

Structural features of the crystalline enzyme are described; the relationship of many of these to the active site of the enzyme are discussed.

Structures of a Blue-Copper Nitrite Reductase and its Substrate-Bound Complex

First crystal structures of a blue-copper nitrite reductase in its oxidized and nitrate-complexed form have been determined.

Structure of Crystal Form IX of Bovine Pancreatic Ribonuclease A

The crystal form of ribonuclease A is unusual in that it diffracts well when dehydrated. Nevertheless, the hydrated structure described herein is similar to that found in other crystal forms.

Procedure to Calculate the Molecular Envelope from a Partial Model

Molecular envelope calculation is a critical step in the density-modification procedure. An improved procedure is proposed to evaluate this envelope when the MR phases are biased by the model.

A Connected Set Algorithm for the Identification of Spatially Contiguous Regions in Crystallographic Envelopes

A simple algorithm is described for the identification of spatially contiguous regions in crystallographic envelopes. All sets of points that are globally connected according to a local connectivity criterion are systematically identified during a single sequential pass through the grid points of the envelope map

Different Tools to Study Interaction Potentials in γ-Crystallin Solutions: Relevance to Crystal Growth

Protein solubility and crystallization are determined in solution by interaction potentials. The tuning with pH of the interactions, measured with osmotic pressure, small-angle X-ray scattering and quasi-elastic light scattering, gives results for two potentials: a coulombic repulsive and a van der Waals attractive potential.

wARP: Improvement and Extension of Crystallographic Phases by Weighted Averaging of Multiple-Refined Dummy Atomic Models

wARP is a procedure for the improvement and extension of crystallographic phases. It can be applied as a subsequent step to any conventional phasing or map-modification technique. Requirements are native data extending to at least 2.5 Å resolution and a map of reasonable quality. In the presented examples wARP resulted in a dramatically better phase set and subsequent map improvement far beyond what is provided by existing methodology.

Electrophoretic mobility and zeta potential of lysozyme crystals

As shown by free-solution capillary electrophoresis, crystals of hen egg-white lysozyme are positively charged because of absorption of H⁺ when suspended in aqueous acid solution. The ζ potential increases from 8 to 24 mV as the pH decreases from 5.70 to 3.59.

Crystallization and preliminary X-ray crystallographic studies of type A influenza virus matrix protein M1

The influenza virus matrix protein M1 has been crystallized. The crystals are in space group P3₁21 with a = 66.17 and b = 135.30 Å.

Crystallization and preliminary X-ray crystallographic analysis of the electron-transferring flavoprotein from Megasphaera elsdenii

The X-ray diffraction data collected from crystals of the electron-transferring flavoprotein from M. elsdenii is consistent with an orthorhombic space group and a very low solvent content.

Structure of apo duck ovotransferrin: the structures of the N and C lobes are in the open form

The crystal structure of apo duck ovotransferrin has been determined. Both lobes are in the open form.

Homology between jacalin and artocarpin from jackfruit (Artocarpus integrifolia) seeds. Partial sequence and preliminary crystallographic studies of artocarpin

Comparison of the partial amino-acid sequence of artocarpin with the known sequence of jacalin and the solution of the crystal structure of the former using the known structure of the latter shows that the two lectins from jackfruit seeds are homologous.

Crystallization of the Fab fragment of the tumour-specific antibody PR1A3

Crystals of the Fab fragment of the tumour-specific antibody PR1A3 diffracting to 2.9 Å, have been obtained by repeated macroseeding.

Purification, crystallization and preliminary X-ray diffraction studies of recombinant calcium-binding domain of the small subunit of porcine calpain

Crystals of the calcium-bound domain VI of porcine calpain have been obtained in space group P2₁ by vapor-diffusion methods. Crystals diffract to 1.9 Å resolution. There are two molecules in the asymmetric unit.

Purification, crystallization and preliminary X-ray analysis of the M.BseCI DNA methyltransferase from Bacillus stearothermophilus

The DNA methyltransferase, M.BseCI from B. stearothermophilus methylates the N6 atom of the 3^' adenine in the sequence 5^'-ATCGAT-3^'. M.BseCI crystals difracting to at least 2.5 Å resolution have been obtained and characterized.

Preliminary crystallographic study on a low molecular weight form of bacterial plasminogen activator staphylokinase

Recombinant Δ10 form of staphylokinase crystallizes in orthorhombic space group C22₁ with unit-cell parameters a = 43.78, b = 59.86 and c = 103.25 Å and one molecule in the asymmetric unit.

Crystallization of the RNA guanylyltransferase of Chlorella virus PBCV-1

RNA guanylyltransferase from Chlorella virus PBCV-1 has been crystallized. Diffraction data have been collected using native, selenomethionine and mercury-derivatized enzyme.

Purification, crystallization and preliminary X-ray analysis of Bacteroides fragilis Zn²⁺ β-lactamase

Crystallization of Bacteroides fragilis Zn²⁺ β-lactamase. Unit-cell dimensions are a = 56.03, b = 43.98 and c = 105.32 Å, β = 112°.

Isocitrate lyase from Aspergillus nidulans: crystallization and X-ray analysis of a glyoxylate cycle enzyme

The tetrameric isocitrate lyase from Aspergillus nidulans has been crystallized in space group P4₂2₁2 with a monomer in the asymmetric unit.

Preliminary X-ray diffraction studies on asparaginyl-tRNA synthetase from Thermus thermophilus. Erratum

Handbook of crystal growth. Vol. 2:Bulk crystal growth. a: Basic techniques; b: Growth mechanisms and dynamics edited by D. T. J. Hurle