Structure of a novel ribosome-inactivating protein from a hemi-parasitic plant inhabiting the northwestern Himalayas

The crystal structure of a ribosome-inactivating protein from Himalayan V. album has been determined at 3.8 Å resolution. The structure has revealed unique structural features of the functionally important sites, including the active site, sugar-binding site, antigenic epitopes and ribosome-recognition site.

Structure of photoactive yellow protein (PYP) E46Q mutant at 1.2 Å resolution suggests how Glu46 controls the spectroscopic and kinetic characteristics of PYP

The crystal structure of photoactive yellow protein E46Q mutant was determined at 1.2 Å resolution, revealing that the hydrogen bond between p-coumaric acid and Glx46 is lengthened by the mutation. The alkali shift in the pK_a, the red shift in the absorption maximum and the rapid photocycle of E46Q can be explained by the weakened hydrogen bond.

Structure of shikimate kinase from Mycobacterium tuberculosis reveals the binding of shikimic acid

The crystal structure of shikimate kinase from Mycobacterium tuberculosis (MtSK) complexed with MgADP and shikimic acid (shikimate) has been determined at 2.3 Å resolution, clearly revealing the amino-acid residues involved in shikimate binding. This is the first three-dimensional structure of shikimate kinase complexed with shikimate.

Structural basis for discrimination between oxyanion substrates or inhibitors in aspartate-β-semi­aldehyde de­hydrogenase

Enzyme structural differences between an alternative substrate and an inhibitor bound at the phosphate site have established the features controlling the oxyanion-specificity of aspartate-β-semialdehyde dehydrogenase.

Purification, crystallization and preliminary X-ray analysis of the N-terminal domain of NO38, a nucleolar protein from Xenopus laevis

NO38 may function as a histone chaperone in Xenopus nucleoli. The conserved N-terminal domain of NO38 has been crystallized as a thermostable pentamer and as a decamer.

Purification, crystallization and preliminary X-ray diffraction analysis of Methanococcus jannaschii TATA box-binding protein (TBP)

TATA box-binding protein (TBP) from M. jannaschii has been crystallized by the hanging-drop vapour-diffusion method. A diffraction data set was collected to 1.9 Å resolution using synchrotron radiation. A molecular-replacement solution was found using the structure of TBP from S. acidocaldarius as a model.

Crystallization and preliminary X-ray diffraction analysis of ferredoxin-NADP(H) reductase from Rhodobacter capsulatus

Ferredoxin-NADP(H) reductase from R. capsulatus has been crystallized by the hanging-drop vapour-diffusion method. The addition of a specific detergent is crucial to stabilize disordered regions in the crystal.

Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase

The coiled-coil domain of dystrophia myotonica kinase has been cloned, overexpressed, purified and crystallized. Phasing information has been obtained by molecular replacement from models lacking sequence similarity and experimentally by MAD from an SeMet-derivative.

Crystallization and preliminary crystallographic analysis of the 2′-aminobi­phenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10

CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by P. resinovorans strain CA10, has been crystallized and diffraction data were collected to 1.9 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL), a crotonase homologue active in phenylpropanoid metabolism

A preliminary crystallographic analysis of the crotonase homologue hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL) is presented.

Crystallization and initial crystallographic analysis of the Kelch domain from human Keap1

The crystallization of the Kelch domain of human Keap1 protein by hanging-drop vapor diffusion is reported in space group P6₅22.

Crystallization and preliminary crystallographic studies of MOMP (major outer membrane protein) from Campylobacter jejuni

In order to determine the three-dimensional structure of this protein and to elucidate the underlying molecular mechanisms, the MOMP from C. jejuni strain 85H has been purified and crystallized by vapour diffusion.

Expression, crystallization and preliminary X-ray crystallographic studies of Klebsiella pneumoniae maltohexaose-producing α-amylase

A recombinant form of K. pneumoniae maltohexaose-producing α-amylase was overexpressed in E. coli, purified to homogeneity and crystallized by the microbatch technique using polyethylene glycol. The crystal diffracted to better than 2.5 Å resolution at 95 K.

Crystallization of parasporin-2, a Bacillus thuringiensis crystal protein with selective cytocidal activity against human cells

An active form of parasporin-2, a B. thuringiensis crystal protein toxic to particular human cells, has been crystallized. Native data have been collected to 2.75 Å resolution.

Crystallization and preliminary X-ray analysis of the Streptomyces olivaceoviridis NgcE binding protein of the ABC transporter for N-acetylglucosamine

The binding protein NgcE of the ABC transporter for N-acetylglucosamine uptake in S. olivaceoviridis was overexpressed and purified. Plate-like crystals were obtained that belonged to either space group P2₁2₁2 or P2₁2₁2₁ and diffracted to 2.2 or 1.8 Å resolution, respectively.

Crystallization and preliminary X-ray characterization of the atypical glutaminyl-tRNA synthetase from Deinococcus radiodurans

The glutaminyl-tRNA synthetase from D. radiodurans possesses an atypical C-terminal domain the function of which remains unknown. Orthorhombic crystals diffracting to 2.3 Å resolution were obtained from overexpressed protein and characterized using synchrotron radiation. The data allowed solution of the structure by molecular replacement.

Expression, purification and preliminary crystallographic characterization of a novel segment from the neurofibromatosis type 1 protein

A novel 31 kDa segment of the neurofibromatosis type 1 protein has been overexpressed in E. coli, purified and crystallized. Various crystal forms were obtained with a maximum resolution of 2.3 Å.

Crystallization and preliminary X-ray diffraction analysis of bacteriophage φ12 packaging factor P7

The bacteriophage φ12 packaging, factor P7 was crystallized, in both full-length and C-terminus truncated forms, P3₂21, a = 75.7, b = 75.7, c = 45.2 Å, α = 90, β = 90, γ = 120°. Cross-linking indicated dimers of P7 and the need of the N-terminus for dimerization.

Preliminary crystallographic characterization of BSAP, an extracellular aminopeptidase from Bacillus subtilis

The double-zinc aminopeptidase from B. subtilis (BSAP) has been crystallized in a hexagonal space group. Complete zinc MAD data at 2.5 Å resolution and a full native data at 2.2 Å resolution were collected.

Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein

Crystals of the fusion core of SARS-CoV spike protein have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 Å resolution at 100 K in-house. The crystals have unit-cell parameters a = 121.2, b = 66.3, c = 70.0 Å, α = γ = 90, β = 107.4° and belong to space group C2.

Crystallization and preliminary X-ray diffraction analysis of the unliganded human growth hormone receptor

The unliganded form of human growth hormone receptor has been expressed, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to space group C222₁ and diffract to 2.5 Å.

Crystallization and preliminary X-ray crystallographic studies of dissimilatory nitrite reductase isolated from Hyphomicrobium denitrificans A3151

Dissimilatory nitrite reductase (CuNIR) isolated from H. denitrificans A3151 exhibits a similar structure to a complex of known green CuNIR and pseudoazurin (or azurin). Crystallographic studies of this enzyme and a mutated form, C260A, which lacks a type I copper ion in the CuNIR-like domain are reported.

Crystallization and preliminary crystallographic studies of human coactosin-like protein (CLP)

The human coactosin-like protein (CLP) has been crystallized by the hanging-drop vapour-diffusion method. Diffraction data have been collected to 1.9 Å resolution.

Escherichia coli stress protein YciF: expression, crystallization and preliminary crystallographic analysis

YciF protein from E. coli was overexpressed and purified. Crystals of YciF in space group R32 diffracted to a resolution of 2.25 Å.

Crystallization and preliminary X-ray crystallographic analysis of a non-specific lipid-transfer protein with antipathogenic activity from Phaseolus mungo

A 9 kDa non-specific lipid-transfer protein (nsLTP) from mung bean (Phaseolus mungo) seeds, displaying antifungal activity, antibacterial activity and lipid-transfer activity, was crystallized at 297 K using ammonium sulfate as a precipitant by means of the hanging-drop vapour-diffusion method.

Crystallization and preliminary X-ray crystallographic analysis of the laminarinase endo-β-1,3-glucanase from Pyrococcus furiosus

Single crystals of P. furiosus LamA have been obtained by the hanging-drop vapour-diffusion method using 2-­methyl-2,4-pentanediol as a precipitant agent. A complete data set has been collected under cryocooling at a synchrotron source.

Crystallization, X-ray diffraction and oligomeric characterization of arginine de­carboxylase from Yersinia pestis, a key polyamine biosynthetic enzyme

The pyridoxal-5′-phosphate-dependent arginine decarboxylase from Y. pestis was crystallized. Crystals diffract to 3.0 Å resolution and belong to the monoclinic space group P2₁, with unit-cell parameters a = 116.5, b = 78.3, c = 147.3 Å, β = 91.7°. Light scattering indicates ADC to be a dimer of 178 kDa in solution; in the crystalline state the data is consistent with two dimers in the asymmetric unit.

Crystallography of the integral membrane protein EmrE from Escherichia coli

The direct-methods Shake-and-Bake program SnB was used to obtain the substructure solution from a mutant of E. coli EmrE using data with limited anomalous difference resolution of 5.5 Å.

SAD at home: solving the structure of oxalate de­carboxylase with the anomalous signal from manganese using X-ray data collected on a home source

The crystal structure of oxalate decarboxylase was solved by manganese SAD using a 2 Å resolution data set collected on a home X-ray source.