issue contents

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047

November 2005 issue

Highlighted illustration

Cover illustration: Ribbon diagram and transparent surface representation of the hexameric assembly of inorganic pyrophosphatase (PPase) from Helicobacter pylori, as viewed along the molecular threefold axis (p. 1459). The structural comparison of hexameric PPase appears to reflect the evolutionary relationship among PPase from bacterial species.

research papers


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Various anomalous signal indicators and their use in the last 50 years are reviewed. A Monte Carlo procedure is described that allows the estimation of a number of these indicators as well as the resulting cosine of the phase error as obtained from experimental SAD phases and the number of significant Bijvoet differences in a SAD data set.


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The crystal structure of inorganic pyrophosphatase from H. pylori was determined at 2.6 Å resolution, revealing that structural flexibility of the loops near the active site is required for catalytic efficiency.

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The ACORN program has been extended to work with data to resolution down to 1.7 Å.

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Although the structure of T6 bovine insulin is nearly identical to that of porcine insulin, the loss of Cδ1 from isoleucine at residue A10 in porcine insulin to produce valine in bovine insulin results in a more efficient packing of the hexamers along the c axis.

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By studying nine crystallographically independent structures of M. viridifaciens sialidase, the relative flexibility of its three domains has been analysed. A detailed study is also presented of the recognition of galactose and lactose by the M. viridifaciens carbohydrate-binding module.

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The X-ray structure of a conserved hypothetical protein of molecular weight 16.3 kDa from M. tuberculosis corresponding to the open reading frame Rv1155 has been solved and refined at 1.8 Å resolution. The structures of flavin mononucleotide (FMN) and pyridoxal 5′-phosphate (PLP) bound separately to Rv1155 have been determined at 2.2 and 1.7 Å resolution, respectively.

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The structure of ATP-dependent phosphoenolpyruvate carboxykinase from T. thermophilus HB8 has been determined at 2.0 Å resolution. This first three-dimensional structure of a thermostable orthologue exhibits the so-called `conformational selection' mechanism of induced fit upon ATP binding. The polypeptide fold contains a bound calcium ion which has been suggested to be important for the thermostability of this enzyme.


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The FINDMOL algorithm is designed to quickly locate contiguous symmetrically unique macromolecular regions from medium- to low-resolution skeletonized electron-density maps. It uses cluster analysis of trace points representing high-density regions. Higher level prior information such as sequence information is not required. In most cases, the resulting arrangement of trace points resembles the contiguous protein macromolecule.

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A limited number of accurate phases were measured for strong low resolution rhombohedral insulin reflections. These phases greatly improved initial map quality and subsequent density modification.

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A simple method for growing protein crystals in thin-wall water-permeable plastic tubing provides greater kinetic control and simplifies setup, handling and crystal retrieval. Tubing thicknesses that give desirable permeability and mechanical properties produce little X-ray scatter and allow rapid in situ flash-cooling.

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Three phasing methods were compared for data sets collected with a Cr Kα X-ray source using loopless data collection. Phasing with OASIS-2004 gave the best results for four standard proteins and the 69 kDa protein TT0570.


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The crystal structure of a truncated, soluble form of human semicarbazide-sensitive amine oxidase, also know as vascular adhesion protein-1, is described.
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