Structure of mouse IP-10, a chemokine

The structure of mouse IP-10 shows a novel tetrameric association.

Building of RNA and DNA double helices into electron density

A method of automatically fitting nucleic acid double helices into electron density of high to medium resolution is presented and tested.

The 1.25 Å resolution structure of phosphoribosyl-ATP pyro­phospho­hydrolase from Mycobacterium tuberculosis

The crystal structure of M. tuberculosis phosphoribosyl-ATP pyrophosphohydrolase, the second enzyme in the histidine-biosynthetic pathway, is presented. The structural and inferred functional relationships between M. tuberculosis phosphoribosyl-ATP pyrophosphohydrolase and other members of the nucleoside-triphosphate pyrophosphatase-fold family are described.

Structure of the recombinant antibody Fab fragment f3p4

The structure of the Fab fragment f3p4 selected from the HuCAL GOLD library is reported.

Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family

The crystal structure of the enzymatically active domain of Ply500 has been solved at 1.8 Å resolution.

Structure of human α-enolase (hENO1), a multifunctional glycolytic enzyme

The crystal structure of human α-enolase (hENO1) has been determined for the first time and discloses distinct surface properties despite its high sequence similarities to other enolases. The surface properties provide an insight into its unique activities, including plasmin(ogen) and DNA binding, which are closely related to its roles in numerous diseases such as metastatic cancer, autoimmune disorders, ischaemia and bacterial infection.

An investigation into the protonation states of the C1 domain of cardiac myosin-binding protein C

An evaluation is made of the protonation states of surface Asp and Glu residues in the C1 domain of cMyBP-C at 1.3 Å resolution. Extrapolations are made of the X-ray diffraction resolution required and/or of the specimen volume required for neutron protein crystallography to determine such states precisely.

Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 Å resolution

Hybrid cluster proteins (HCP), whose function has yet to be determined, possess a similar fold to carbon monoxide dehydrogenases which also have a related metal–sulfur cluster at the equivalent hybrid cluster position. In HCP the precise nature of the hybrid cluster appears to be dependent on the absence or presence of oxygen during the purification and crystallization procedures.

Insights into the structural basis of substrate recognition by histidinol-phosphate aminotransferase from Corynebacterium glutamicum

The crystal structures of apo histidinol-phosphate aminotransferase from C. glutamicum, of the internal PLP aldimine adduct and of a pyridoxamine 5-phosphate–enzyme complex were determined at resolutions of 2.2, 2.1 and 1.8 Å, respectively. The residues lining the substrate-binding pocket were studied by site-directed mutagenesis.

Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites

Microporous molecular sieves were used as a hetero-epitaxic nucleant for protein crystallization.

Structure of an archaeal alanine:glyoxylate aminotransferase

The crystal structure of an archaeal alanine:glyoxylate aminotransferase which has an amino-acid sequence that shows no similarity to any other known alanine:glyoxylate aminotransferases was determined.

Structural characterization of a human Fc fragment engineered for lack of effector functions

Human Fc fragments containing the L234F/L235E/P331S triple mutation exhibit a dramatic decrease in their binding to several effector molecules (CD64, CD32A, CD16 and C1q). The three-dimensional structure of such a mutated fragment reveals that these broad-ranging functional effects are not caused by major structural rearrangements in the Fc moiety.