An additional C-terminal loop in endonuclease IV, an apurinic/apyrimidinic endonuclease, controls binding affinity to DNA

The crystal structures of long-type and short-type endonucleases IV were determined. These structures revealed that an additional C-terminal loop in the long type was responsible for its higher DNA-binding affinity.

A crystallographic fragment screen identifies cinnamic acid derivatives as starting points for potent Pim-1 inhibitors

The presented hits from a crystallographic fragment screen provide direct structural evidence for the primary tenet of fragment-based lead discovery: while low in potency, fragments form high-quality interactions and therefore present valid starting points for further optimization into drug-like molecules.

The structure of Bacillus subtilis SPβ prophage dUTPase and its complexes with two nucleotides

The structure of the Bacillus subtilis SPβ prophage dUTPase is described for the apo-enzyme and complexes with dUDP and the trinucleotide analogue dUpNHpp. The triphosphate moiety in the gauche conformation is needed to trigger conformational changes in the enzyme, including stacking of the Phe-lid over the uracil.

JBluIce–EPICS control system for macromolecular crystallography

A fast, user-friendly and easily extensible beamline-control system based on a combination of Java Eclipse RCP and EPICS and featuring a user interface similar to that of the SSRL BluIce has been developed at the GM/CA-CAT macromolecular crystallography beamlines in Sector 23 of the Advanced Photon Source.

Understanding the polymorphic behaviour of a mutant of the α-spectrin SH3 domain by means of two 1.1 Å resolution structures

Two polymorphic crystals of the α-spectrin SH3 domain were obtained using the same crystallization conditions. The hexagonal polymorph represents a more thermodynamically stable form, whereas the orthorhombic form is the kinetically favoured form.

Structural characterization of CalO1: a putative orsellinic acid methyltransferase in the calicheamicin-biosynthetic pathway

The structural characterization of CalO1 is reported.

Structure of the mouse galectin-4 N-terminal carbohydrate-recognition domain reveals the mechanism of oligosaccharide recognition

The crystal structure of the mouse galectin-4 N-terminal domain CRD1 in complex with lactose was determined at 2.1 Å resolution. Lactose-binding studies together with the structural analysis provide a basis for understanding the mechanism for the recognition of complex sugars.

Structure of the sortase AcSrtC-1 from Actinomyces oris

The structure of the fimbriae-associated sortase AcSrtC-1 from A. oris has been determined to 2.4 Å resolution.

Modification of the sugar specificity of a plant lectin: structural studies on a point mutant of Erythrina corallodendron lectin

The crystal structures of a mutant of E. corallodendron lectin in complex with galactose and N-acetylgalactosamine provide a structural view of an engineered carbohydrate-binding site of a legume lectin.

The use of low-resolution phasing followed by phase extension from 7.6 to 2.5 Å resolution with noncrystallographic symmetry to solve the structure of a bacteriophage capsid protein

Using hybrid methods to solve the phase problem for a viral protein showing non-crystallographic symmetry.