Structures of Brucella ovis leucine-, isoleucine-, valine-, threonine- and alanine-binding protein reveal a conformationally flexible peptide-binding cavity

Brucella ovis leucine-, isoleucine-, valine-, threonine- and alanine-binding protein structures have a prototypical bacterial periplasmic amino acid-binding protein topology with a conformationally flexible peptide-binding cavity in the absence of peptide.

Crystallographic fragment screen of the c-di-AMP-synthesizing enzyme CdaA from Bacillus subtilis

The crystal structure determination of CdaA enzymes from Streptococcus pneumoniae, Bacillus subtilis and Enterococcus faecium is reported. Additionally, the structural results of a fragment screen of B. subtilis CdaA are presented, along with a subsequent in silico drug-repurposing screen conducted using the OpenEye suite.

Enhancing the apo protein tyrosine phosphatase non-receptor type 2 crystal soaking strategy through inhibitor-accessible binding sites

Protein tyrosine phosphatase non-receptor type 2 (PTPN2), a known target for cancer immunotherapy, was crystallized with novel crystal packing, resulting in a true apo PTPN2 crystal structure with an unbound active site. Inhibitor-bound PTPN2 crystal structures were obtained by soaking these apo crystals with an active-site inhibitor and an allosteric inhibitor.

Crystal structure of the GDP-bound human M-RAS protein in two crystal forms

The crystal structure of GDP-bound human M-RAS protein is presented with two forms of crystal packing.

Crystal structure of the Rib domain of the cell-wall-anchored surface protein from Limosilactobacillus reuteri

The high-resolution structure of a Rib domain derived from L. reuteri reveals a significant structural variant of the canonical Ig-like fold.