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![[Figure 8]](hi5555fig8mag.jpg)
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Figure 8
The ability of the technique to provide reliable data from impure samples and mixtures was illustrated by a run with a sample containing three components: cytochrome c, Rg = 1.3 nm, bovine serum albumin, Rg = 3.0 nm, and blue dextran, a carbohydrate molecule of very high molecular mass (>1 MDa) and uncertain but undoubtedly high Rg. This latter compound is a common chromatographic standard used to represent limitingly large molecules, and here serves as a proxy for aggregates. It can be seen that reliable plateau regions were observed for both proteins, even though the chromatographic separation was marginal. The Rg data for the blue dextran are not reliable, since our experimental set-up did not have the angular resolution to fully capture the Guinier region for molecules of this size. As such, the open circles do not satisfy the criterion Rgqmax = 1.3 used everywhere else in this paper, and are presented only as guide to the increasing size of this molecule, as would be expected from its chromatographic position. Typically, in this region, Rgqmax was ∼2.5. |
![[Figure 8]](hi5555fig8.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
