Image-processing methods for electron microscopy of biological specimens

The focused issue on Image-processing methods for electron microscopy of biological specimens is introduced. The virtual issue is available at https://journals.iucr.org/special_issues/2025/imageprocessing.

Mechanistic insight into O=O bond formation upon model-independent visualization of the coordination geometry and ligand composition of Mn₄Ca cofactor in dark-adapted photosystem II structures

Model-independent visualization of oxygen-evolving center-omit electron-density maps of dark-adapted photosystem II reveals the inner workings of this four-step/four-chamber combustion engine running in a reverse direction.