Separating the effects of temperature and absorbed X-ray dose on unit-cell volume

The nature of the expansion of the crystal unit-cell volume with absorbed dose and with temperature was examined for three different proteins.

Single-crystal X-ray and in crystallo XANES studies resolve cobalt coordination in cobalamin and rule out radiation-induced ligand loss in human intrinsic factor

High-resolution crystallography combined with in crystallo Co K-edge XANES shows that cobalamin retains octahedral cobalt coordination in both oxidized and reduced states. These results rule out radiation-induced Co–CN cleavage as the cause of the missing axial ligand in intrinsic factor-bound cobalamin.