Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major

The crystal structure of a conserved hypothetical protein from L. major, Pfam sequence family PF04543, structural genomics target ID Lmaj006129AAA, has been determined at a resolution of 1.6 Å.

Structure of the conserved hypothetical protein MAL13P1.257 from Plasmodium falciparum

The crystal structure of a conserved hypothetical protein, MAL13P1.257 from P. falciparum, has been determined at 2.17 Å resolution. The structure represents a new protein fold and is the first structural representative for Pfam sequence family PF05907.

Crystallization and preliminary crystallographic analysis of selenome­thionine-labelled progesterone 5β-reductase from Digitalis lanata Ehrh

Progesterone 5β-reductase is the first stereospecific enzyme in the pathway for the synthesis of cardenolides. To elucidate the structural mechanism of this reaction, we crystallized the selenomethionine-labelled enzyme from D. lanata and report the preliminary analysis of a MAD data set collected from these crystals.

Crystallization and preliminary X-ray analysis of rat SHPS-1

The ligand-binding domain of rat SHPS-1 was purified and crystallized using the vapour-diffusion method with the solution-stirring technique.

Crystallization of Escherichia coli CdtB, the biologically active subunit of cytolethal distending toxin

Cytolethal distending toxin subunit CdtB from E. coli strain 9142-88 was purified and crystallized. Crystals belonging to space group P2₁2₁2₁ diffract to a resolution of 1.72 Å.

Capillary crystallization and molecular-replacement solution of haemoglobin II from the clam Lucina pectinata

The haemoglobin II from the clam L. pectinata has been crystallized using counter-diffusion in single capillary in the presence of agarose to improve crystal quality. Initial phases have been obtained by molecular replacement.

Crystallization of the class IV adenylyl cyclase from Yersinia pestis

The class IV adenylyl cyclase from Y. pestis has been crystallized in an orthorhombic form suitable for structure determination.

Crystallization and preliminary characterization of a highly thermostable lectin from Trichosanthes dioica and comparison with other Trichosanthes lectins

A lectin from Trichosanthes dioica seeds has been purified and crystallized using 25%(w/v) PEG 2K MME, 0.2 M ammonium acetate, 0.1 M Tris–HCl pH 8.5 and 50 µl 0.5%(w/v) n-octyl β-D-glucopyranoside as thick needles belonging to hexagonal space group P6₄.

Expression, purification, crystallization and preliminary X-ray analysis of a C-terminal fragment of the Epstein–Barr virus ZEBRA protein

A C-terminal fragment of the Epstein–Barr virus lytic switch protein ZEBRA has been crystallized in complex with DNA.

Crystallization and preliminary X-ray analysis of cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens

A novel cytochrome c nitrite reductase (TvNiR) has been isolated from the haloalkalophilic bacterium T. nitratireducens. TvNiR crystals were grown by the hanging-drop vapour-diffusion technique.

Crystallization and initial crystal characterization of the C-terminal phosphoglycerate mutase homology domain of Sts-1

The C-terminal domain of the signaling protein Sts-1 has been expressed in bacteria, purified and crystallized and diffraction data sets have been collected from two crystal forms.

Crystallization and preliminary crystallographic studies of human indole­amine 2,3-dioxygenase

Human indoleamine 2,3-dioxygenase, a haem-containing dioxygenase, was crystallized. The crystal diffracted to 2.3 Å resolution.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of human ARH3, the first eukaryotic protein-ADP-ribosylhydrolase

Human ADP-ribosylhydrolase 3 (ARH3), which has been identified as an ARH by a sequence-similarity search and which cleaves the glycosidic bond of ADP-ribose attached to a protein, has been cloned, expressed, purified and crystallized in two different space groups.

Overexpression, purification, crystallization and data collection of Sulfolobus solfataricus Sso6206, a novel highly conserved protein

The novel protein Sso6206 has been crystallized; interestingly, the protein may form large multi-subunit oligomers.

Biosynthesis of a 3,6-di­deoxyhexose: crystallization and X-ray diffraction of CDP-6-de­oxy-L-threo-D-glycero-4-hexulose-3-dehydrase (E₁) for ascarylose biosynthesis

E₁ dehydrase, which is important in the biosynthesis of the 3,6-dideoxy sugar ascarylose and is the only known PMP-containing enzyme to carry out one-electron chemistry, has been crystallized and diffracted to 1.9 Å.

Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds

A lectin from C. roseum seeds (CRL) has been purified, characterized and crystallized.

A single point mutation changes the crystallization behavior of Mycoplasma arthritidis-derived mitogen

The mutagenesis, crystallization and preliminary crystallographic analysis of M. arthritidis-derived mitogen is described.

Expression, purification and crystallization of Streptococcus dysgalactiae-derived mitogen

S. dysgalactiae-derived mitogen, a superantigen, was crystallized. Crystals diffract to 2.4 Å at a synchrotron-radiation source and belong to space group P3/P3₁/P3₂, with unit-cell parameters a = b = 52.7, c = 62.4 Å, γ = 120° and one molecule in the crystallographic asymmetric unit.

Crystallization and halide phasing of the C-terminal domain of human KIN17

Expression, purification, crystallization and phasing procedure are reported for the C-terminal domain of human KIN17.

Crystallization and preliminary X-ray diffraction analysis of iron regulatory protein 1 in complex with ferritin IRE RNA

The iron regulatory protein IRP1 has been crystallized in a complex with ferritin IRE RNA and a complete data set has been collected to 2.8 Å resolution.

Stoichiometric complex formation by proliferating cell nuclear antigen (PCNA) and its interacting protein: purification and crystallization of the DNA polymerase and PCNA monomer mutant complex from Pyrococcus furiosus

A stable stoichiometric complex of archaeal DNA polymerase with proliferating cell nuclear antigen (PCNA) was formed using a PCNA monomer mutant and the complex was successfully crystallized.

Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis

Raucaffricine glucosidase, an enzyme involved in the biosynthesis of monoterpenoid indole alkaloids in the plant Rauvolfia serpentina, was crystallized by the hanging-drop vapour-diffusion method using PEG4000 as precipitant. The crystals diffract to 2.3 Å resolution and belong to space group I222.

Purification, crystallization and preliminary characterization of a putative LmbE-like de­acetylase from Bacillus cereus

The BC1534 protein from B. cereus was purified and crystallized and a native X-ray diffraction data set was collected to 2.5 Å using synchrotron radiation.

Preliminary X-ray crystallographic studies of yeast mitochondrial protein Tom70p

Tom70p is an important translocase of the outer membrane complex member and a major surface receptor of the protein-translocation machinery in the outer mitochondrial membrane. To investigate the mechanism by which Tom70p functions to deliver the mitochondrial protein precursors, the cytosolic fragment of yeast Tom70p (cTom70p) has been crystallized.

Expression, crystallization and preliminary crystallographic analysis of SufE (XAC2355) from Xanthomonas axonopodis pv. citri

Cloning, expression, purification, crystallization and data collection are reported for a member of the SufE family of proteins involved in the biosynthesis of Fe–S clusters in prokaryotes. Diffraction data were collected to 1.9 Å resolution and an interpretable electron-density map has been obtained by molecular replacement.

Crystallization and X-ray diffraction analysis of salicylate synthase, a chorismate-utilizing enyme involved in siderophore biosynthesis

Salicylate synthase, which catalyzes the first step in the synthesis of the siderophore yersiniabactin, has been crystallized. Diffraction data have been collected to 2.5 Å.

Crystallization and preliminary X-ray crystallographic analysis of biodegradative threonine deaminase (TdcB) from Salmonella typhimurium

S. typhimurium biodegradative threonine deaminase (TdcB), a member of the β-family of PLP-dependent enzymes, has been overexpressed, purified and crystallized in three different crystal forms using the hanging-drop vapour-diffusion method.

Crystallization and preliminary X-ray analysis of a bacterial L-amino-acid oxidase from Rhodococcus opacus

The crystallization and preliminary X-ray analysis of a bacterial L-amino acid oxidase from R. opacus is described. The homodimeric protein contains one molecule of non-covalently bound FAD per monomer. Crystals with good diffraction properties were grown in two different orthorhombic space groups (P2₁2₁2₁ and C222₁).

Crystallization and preliminary X-ray crystallographic investigations on a βγ-crystallin domain of absent in melanoma 1 (AIM1), a protein from Homo sapiens

The crystallization and preliminary X-ray diffraction analysis of AIM1g1, a βγ-crystallin domain of absent in melanoma (AIM1) protein from H. sapiens, is reported.

Crystallization and X-ray diffraction analysis of the complement component-3 (C3) inhibitory domain of Efb from Staphylococcus aureus

The crystallization and results of multiwavelength anomalous diffraction studies of a recombinant C3-inhibitory fragment of Efb from S. aureus are reported.

Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri

The molybdate-binding protein (ModA) from X. axonopodis pv. citri was crystallized with sodium molybdate in the presence of PEG or sulfate. The crystal diffracted to a maximum resolution of 1.7 Å and belongs to the orthorhombic space group C222_1, with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 Å.

Purification, crystallization and preliminary X-ray structural studies of a 7.2 kDa cytotoxin isolated from the venom of Daboia russelli russelli of the Viperidae family

A cytotoxin from Indian Russell's viper (D. russelli russelli) venom having multifunctional activity has been crystallized in space group P4₁. Larger crystals diffracted to 1.5 Å but were found to be twinned; preliminary data were therefore collected (2.93 Å) from a smaller crystal.

Expression, purification, crystallization and preliminary diffraction studies of the mammalian DAG kinase homologue YegS from Escherichia coli

The overexpression, crystallization and preliminary diffraction analysis of E. coli YegS are reported.

Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins

Recombinant His-tagged XendoU, a eukaryotic endoribonuclease, appeared to aggregate in the presence of divalent cations. Monodisperse protein which yielded crystals diffracting to 2.2 Å was obtained by addition of EDTA.

Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle

A monodisperse truncation mutant of MxiH, the subunit of the S. flexneri type III secretion system needle, has been crystallized. SeMet derivatives and a uranyl derivative have undergone preliminary crystallographic analysis.

A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin

Neutron diffraction data of hydrogenated recombinant urate oxidase enzyme (Rasburicase), complexed with a purine-type inhibitor 8-azaxanthin, was collected to 2.1 Å resolution from a crystal grown in D₂O by careful control and optimization of crystallization conditions via knowledge of the phase diagram. Deuterium atoms were clearly seen in the neutron-scattering density map.

Crystallization and preliminary X-ray crystallographic analysis of a highly stable mutant V107A of gluta­thione transferase from Anopheles dirus in complex with gluta­thione

A crystal of an engineered glutathione transferase adgstD4-4 (V107A) from A. dirus, which possesses an enzyme stability that is 32 times greater than the wild type, diffracted to 2.47 Å resolution in space group P3₂21.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii. Erratum

An erratum to the paper by Inagaki et al. [(2006), Acta Cryst. F62, 169–171].