A C-terminal di­sulfide bond in the copper-containing amine oxidase from pea seedlings violates the twofold symmetry of the molecular dimer

In a new crystal form of PSAO, the homodimeric molecules lack twofold symmetry and the C-termini of the two protomers are linked by a previously unrevealed disulfide bond.

The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing

The amidase from G. pallidus RAPc8, a moderate thermophile, converts amides to the corresponding acids and ammonia and has application as an industrial catalyst. RAPc8 amidase has been cloned, expressed and purified, and then crystallized using the hanging-drop vapour-diffusion method.

Structure of HLA-A*1101 in complex with a hepatitis B peptide homologue

The structure of HLA-A*1101 in complex with a HBV peptide homologue is presented and discussed in the context of vaccine design.

The 2.1 Å structure of Aerococcus viridans L-lactate oxidase (LOX)

The crystal structure of L-lactate oxidase (LOX) from A. viridans at 2.1 Å resolution.

Structure of the second domain of the Bacillus subtilis DEAD-box RNA helicase YxiN

The structure of the second helicase domain of the B. subtilis YxiN protein, a DEAD-box RNA helicase, is presented.

The structure of Pyrococcus horikoshii 2′-5′ RNA ligase at 1.94 Å resolution reveals a possible open form with a wider active-site cleft

The crystal structure of the 2′-5′ RNA ligase PH0099 from P. horikoshii OT3 was solved at 1.94 Å resolution. The molecule has a bilobal α+β arrangement with two antiparallel β-sheets constituting a V-shaped active-site cleft, as found in other members of the 2H phosphoesterase superfamily.

The molecular structure of Rv1873, a conserved hypothetical protein from Mycobacterium tuberculosis, at 1.38 Å resolution

SIRAS phasing techniques have been employed to determine the structure Mycobacterium tuberculosis Rv1873 at a resolution of 1.38 Å. We show that this conserved hypothetical protein of unknown function shows limited structural similarity to other molecules.

Purification, crystallization and preliminary X-ray diffraction studies of UDP-N-acetylglucosamine pyro­phosphorylase from Candida albicans

UDP-N-acetylglucosamine pyrophosphorylase was purified and crystallized and X-ray diffraction data were collected to 2.3 Å resolution.

Crystallization and X-ray diffraction analysis of 6-amino­hexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72

Crystals of 6-aminohexanoate-cyclic-dimer hydrolase were obtained by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. Diffraction data from native and mercury-derivative crystals were collected to resolutions of 1.90 and 2.06 Å, respectively.

Crystallization and preliminary X-ray diffraction studies of the terminal oxygenase component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177

The terminal oxygenase component of carbazole 1,9a-dioxygenase from N. aromaticivorans IC177 was crystallized and diffraction data were collected to 2.30 Å resolution.

Crystallization and preliminary X-ray data of the FadA adhesin from Fusobacterium nucleatum

The FadA adhesin from F. nucleatum, which is involved in bacterial attachment and invasion of human oral epithelial cells, has been crystallized in space group P6₁ or P6₅, and X-ray data have been collected to 1.9 Å resolution.

Expression, crystallization and preliminary crystallographic studies of a novel bifunctional N-acetylglutamate synthase/kinase from Xanthomonas campestris homologous to vertebrate N-acetylglutamate synthase

Expression, crystallization and preliminary X-ray diffraction studies of a novel bifunctional N-acetylglutamate synthase/kinase from X. campestris homologous to vertebrate N-acetylglutamate synthase are reported.

Crystallization and preliminary crystallographic studies of the recombinant di­hydropyrimidinase from Sinorhizobium meliloti CECT4114

The dihydropyrimidinase from S. meliloti CECT4114, with activity towards both hydantoin and dihydrouracil substrates, was crystallized, and diffraction data were collected to 1.85 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli

A water-soluble mutant of the outer membrane lipoprotein NlpE has been overexpressed, purified and crystallized. Diffraction data from two crystal forms obtained under two different conditions were collected to 2.8 and 3.0 Å resolution and processed in space groups P4₃2₁2 and C2, respectively.

Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor

Uracil N-glycosylase from M. tuberculosis has been crystallized in complex with a proteinaceous inhibitor (Ugi) and X-ray diffraction data have been collected.

Purification, crystallization and preliminary X-ray analysis of truncated and mutant forms of VP4 protease from infectious pancreatic necrosis virus

Various truncated and mutant forms of the protease VP4 from infectious pancreatic necrosis virus were used to generate two different crystal forms of VP4 which diffracted to beyond 2.4 Å resolution.

Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya

The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å.

Crystallization and preliminary X-ray analysis of a native human tRNA synthetase whose allelic variants are associated with Charcot–Marie–Tooth disease

Crystallization and preliminary X-ray analysis of a native human tRNA synthetase whose allelic variants are associated with Charcot–Marie–Tooth Disease.

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of pseudo death-effector domain of HIPPI, a molecular partner of Huntingtin-interacting protein HIP-1

A pseudo death-effector domain (pDED) of HIPPI, a partner of Huntingtin-interacting protein HIP1, has been cloned, overexpressed and crystallized. The crystals of pDED-HIPPI diffracted to 2.2 Å.

Cloning, expression, crystallization and preliminary crystallographic analysis of a pentapeptide-repeat protein (Rfr23) from the bacterium Cyanothece 51142

A construct of the Cyanothece 51142 pentapeptide-repeat protein Rfr23 (Thr27–Asp174) has been cloned, overexpressed in E. coli and purified. Diffraction data that extend to 2.5 Å and preliminary analyses are reported for selenomethionine-labeled crystals that belong to space group I4₁.

Combining site-specific mutagenesis and seeding as a strategy to crystallize `difficult' proteins: the case of Staphylococcus aureus thioredoxin

S. aureus thioredoxin was crystallized using a combination of seeding and site-specific mutagenesis.

Crystallization and preliminary X-ray diffraction studies on the bicupin YwfC from Bacillus subtilis

The bicupin YwfC from B. subtilis was crystallized in two crystal forms and diffraction data were collected to 2.2 Å resolution.

Crystallization and preliminary X-ray analysis of XC1015, a histidine triad-like protein from Xanthomonas campestris

A HIT-like protein from the plant pathogen X. campestris pathovar campestris has been overexpressed in E. coli, purified and crystallized. The crystals diffract to 1.3 Å.

Overexpression, purification, crystallization and preliminary X-ray cystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1

Preliminary X-ray crystallographic study of a proline-specific aminopepitdase from Aneurinibacillus sp, strain AM-1 was carried out.

Purification, crystallization and preliminary crystallographic studies on 2-de­hydro-3-deoxygalactarate aldolase from Leptospira interrogans

Preliminary crystallographic studies on 2-dehydro-3-deoxygalactarate aldolase from L. interrogans.

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 1

Crystals of baculovirus-expressed adeno-associated virus serotype 1 (AAV1) capsids have been grown in the rhombohedral space group R32 (unit-cell parameters a = 254.7 Å, α = 62.3°) and shown to diffract X-rays to at least 2.5 Å resolution.

Crystallization and preliminary X-ray crystallographic studies of the oxidative-stress sensor SoxR and its complex with DNA

The crystallization and preliminary X-ray analysis of SoxR from E. coli and its complex with DNA are reported.

N-terminal truncation enables crystallization of the receptor-binding domain of the FedF bacterial adhesin

The N-terminal receptor-binding domain of the FedF adhesin from enterotoxigenic E. coli has been crystallized. This required the deletion of its first 14 residues, which are also cleaved off naturally.

Crystallization and preliminary X-ray analysis of human MTH1 complexed with two oxidized nucleotides, 8-oxo-dGMP and 2-oxo-dATP

The complexes of human MTH1 with two oxidized nucleotides, 8-oxo-dGMP and 2-oxo-dATP, were crystallized. The crystals diffracted to 1.95 and 2.22 Å resolution, respectively.

Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants

Perakine reductase, a novel member of the aldo-keto reductase enzyme superfamily of higher plants, is involved in the biosynthesis of monoterpenoid indole alkaloids in the Indian medicinal plant Rauvolfia serpentina. The enzyme has been crystallized in C-centered orthorhombic space group and diffracts to 2.0 Å resolution.

Crystallization and preliminary X-ray characterization of two thermostable DNA nucleases

Two thermostable DNA nucleases from archaea were crystallized in different space groups; the crystals were suitable for X-ray analysis.

Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study

A study on the crystallization of ornithine acetyltransferase from yeast, catalysing the fifth step in microbial arginine synthesis, is presented. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either batch or hanging-drop techniques.

Crystallization and preliminary X-ray analysis of an alditol oxidase from Streptomyces coelicolor A3(2)

Alditol oxidase oxidizes a range of alditols into the corresponding aldoses and is an interesting candidate for biotechnological applications. Crystals of alditol oxidase from S. coelicolor A3(2) were obtained by the hanging-drop vapour-diffusion method and diffracted to 1.1 Å resolution.