Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis

The crystal structure of MtSK in complex with ADP shows conformational changes caused by the absence of the shikimate molecule, and the effect of chloride ions on the nucleotide binding site, and the crystal structure of MtSK in absence of magnesium shows its effect on the shikimate binding site.

Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus

The crystal structure of the catalytic domain of a chitinase from P. furiosus is reported.

Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with its cognate DNA

The DNA methyltransferase M.BseCI from B. stearothermophilus was crystallized as a complex with its cognate DNA. Crystals belong to space group P6 and diffract to 2.5 Å resolution at a synchrotron source.

Purification, crystallization and preliminary crystallographic analysis of archaeal 6-pyruvoyl tetra­hydrobiopterin synthase homologue PH0634 from Pyrococcus horikoshii OT3

An archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue from P. horikoshii OT3 was overexpressed as native and selenomethionine-substituted protein, purified and crystallized. The native and selenomethionine-derivative crystals are isomorphous and diffract X-rays to 2.1 and 2.9 Å resolution, respectively.

Expression, purification and preliminary crystallographic studies on the catalytic region of the nonreceptor tyrosine kinase Fes

The catalytic domain of human Fes tyrosine kinase has been cloned, expressed, purified and crystallized.

Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1

The caspase-recruitment domain of the cytosolic pathogen receptor Nod1 was crystallized. X-ray diffraction data were collected to 1.9 Å resolution.

Preliminary crystallographic analysis of avian infectious bronchitis virus main protease

The avian infectious bronchitis virus main protease has been crystallized; crystals diffract to 2.7 Å resolution.

Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

The molybdenum-cofactor biosynthesis protein C from T. thermophilus has been crystallized in two different space groups, P2₁ and R32; the crystals diffracted to 1.9 and 1.75 Å resolution, respectively.

Preliminary X-ray analysis of XC5848, a hypothetical ORFan protein with an Sm-like motif from Xanthomonas campestris

A conserved hypothetical protein Se-XC5848 from X. campestris pv. campestris has been overexpressed in E. coli, purified and crystallized. Crystals obtained from the purified recombinant protein diffracted to a resolution of 1.68 Å.

Preliminary structural studies of the transcriptional regulator CmeR from Campylobacter jejuni

The transcriptional regulator CmeR from C. jejuni has been purified and crystallized and X-ray diffraction data have been collected to a resolution of 2.2 Å.

Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-secreted periplasmic protein with a potential role in iron transport

The crystallization and structure determination of the apo form of a novel haem-containing Tat substrate, YcdB from E. coli, has been solved to 2.0 Å resolution. The preliminary structure shows similarity to other haem-dependent peroxidases, despite low sequence homology.

Preliminary time-of-flight neutron diffraction study on diiso­propyl fluorophosphatase (DFPase) from Loligo vulgaris

Diisopropyl fluorophosphatase (DFPase) effectively hydrolyzes a number of organophosphorus nerve agents, including sarin, cyclohexylsarin, soman and tabun. Neutron diffraction data have been collected from DFPase crystals to 2.2 Å resolution in an effort to gain further insight into the mechanism of this enzyme.

Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNA^Gly acceptor-stem microhelix

In order to investigate the identity elements of the E. coli tRNA^Gly/GlyRS class II system, a tRNA^Gly acceptor-stem microhelix was crystallized and a data set was collected to 2.0 Å resolution using synchrotron radiation.

Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tails of adhesion molecules CD43 and PSGL-1

The radixin FERM domain has been crystallized in complex with CD43 and PSGL-1 peptides. Diffraction data sets were collected from the complexes to 2.9 and 2.8 Å resolution, respectively.

Exploration of triple-helical fragments: crystallization and preliminary X-ray diffraction of d(TGGCCTTAAGG)

The crystallization and X-ray data collection of the undecamer d(TGGCCTTAAGG) are reported.

Purification, crystallization and preliminary crystallographic analysis of the putative thi­amine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3

The putative thiamine-biosynthesis protein PH1313 from P. horikoshii OT3 was overexpressed, purified and crystallized. The crystals belong to space group P2₁2₁2₁ and diffract X-rays to 1.9 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of two N-terminal fragments of the DNA-cleavage domain of topoisomerase IV from Staphylococcus aureus. Corrigendum

A corrigendum to the paper by Carr et al. (2006>), Acta Cryst. F62, 1164–1167.