Oligomerization of BenM, a LysR-type transcriptional regulator: structural basis for the aggregation of proteins in this family

A general tetramerization and oligomerization scheme is proposed for BenM and other LysR-type transcriptional regulators based on two newly characterized structures of the effector-binding domain of BenM. An analysis of these structures in the light of other LysR-type structures may explain the general solubility problems associated with this protein family.

Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus

Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 Å.

Crystallization of human nicotinamide phosphoribosyltransferase

Human nicotinamide phosphoribosyltransferase has been crystallized using microseeding methods and X-ray diffraction data have been collected at 2.0 Å resolution.

Crystallization and diffraction data of 1H-3-hy­droxy-4-oxo­quinoline 2,4-dioxygenase: a cofactor-free oxygenase of the α/β-hydrolase family

Preliminary crystallographic data for 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from P. putida 33/1 are reported.

Crystallization and preliminary X-ray analysis of 1H-3-hy­droxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter nitroguajacolicus Rü61a: a cofactor-devoid dioxygenase of the α/β-hydrolase-fold superfamily

Preliminary crystallographic data are reported for 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) from A. nitroguajacolicus Rü61a.

Humidity control as a strategy for lattice optimization applied to crystals of HLA-A*1101 complexed with variant peptides from dengue virus

Crystals of an MHC class I molecule bound to naturally occurring peptide variants from the dengue virus NS3 protein contained high levels of solvent and required optimization of cryoprotectant and dehydration protocols for each complex to yield well ordered diffraction, a process facilitated by the use of a free-mounting system.

Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of L-arabinose metabolism

L-2-Keto-3-deoxyarabonate dehydratase was overexpressed, purified and crystallized at 291 K using the hanging-drop vapour-diffusion method.

Crystallization and X-ray analysis of the salmon-egg lectin SEL24K

The 24 kDa egg lectin of Chinook salmon (Oncorhynchus tshawytscha) was purified by affinity chromatography from salmon eggs and crystallized by the hanging-drop vapor-diffusion method using 15/4 EO/OH (pentaerythritol ethoxylate) as a precipitant.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of Thermus aquaticus succinyl-CoA synthetase

Attempts to crystallize succinyl-CoA synthetase from the thermophile T. aquaticus were thwarted by proteolysis of the β-subunit and preferential crystallization of a truncated form. Crystals of the full-length enzyme were grown after the purification protocol was modified to include frequent additions of protease inhibitors.

Crystallization and preliminary X-ray diffraction data of the rat histone H1⁰ globular domain

Crystals of the rat histone H1⁰ globular domain have been grown in the trigonal space group P3₁21 and diffracted to a resolution of 1.98 Å. Molecular-replacement solutions were identified and refinement has been started.

Crystallization and preliminary X-ray analysis of a RecB-family nuclease from the archaeon Pyrococcus abyssi

A RecB-like nuclease from the archaeon Pyrococcus abyssi was expressed, purified and crystallized. The crystals belong to the orthorhombic space group C222₁ with a = 81.5, b = 159.8, c = 100.8 Å, and a native data set was collected to 2.65 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotido­hydrolase from Arabidopsis thaliana

The first crystallization of deoxyuridine triphosphate nucleotidohydrolase from plant, Arabidopsis thaliana, has been performed. An additive, taurine, was effective in producing the single crystal.

Purification, crystallization and preliminary X-ray diffraction studies of a putative UDP-N-acetyl-D-mannosamine de­hydrogenase from Pyrococcus horikoshii OT3

A putative UDP-N-acetyl-D-mannosamine dehydrogenase from P. horikoshii OT3 has been crystallized in space group P2₁, with unit-cell parameters a = 80.28, b = 69.24, c = 83.10 Å, β = 114.4°. X-ray diffraction data have been collected to 1.80 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK

The cloning, expression, purification and crystallization of wild-type AlgK from P. fluorescens is described. The crystals diffract to 2.5 Å resolution.

Crystallization and preliminary X-ray analysis of the mRNA-binding domain of elongation factor SelB from Escherichia coli in complex with RNA

The mRNA-binding domain of E. coli selenocysteine-specific elongation factor SelB (residues 478–614; SelB-WH3/4) was overproduced in E. coli and its cognate mRNA ligand, 23 nucleotides of the SECIS RNA hairpin, was prepared by in vitro transcription. The purified SelB-WH3/4–SECIS RNA complex crystallized in space group C2 and diffracted to 2.3 Å.

Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase from Rhodopseudomonas palustris CGA009

Palustrisredoxin reductase (RPA3782, PuR), a flavin-dependent ferredoxin reductase, is an essential component of the Class I cytochrome P450 systems in Rhodopseudomonas palustris CGA009. Crystals of PuR that diffract to 2.2 Å resolution have been obtained.

Crystallization of the avian reovirus double-stranded RNA-binding and core protein σA

The avian reovirus double-stranded RNA-binding and core protein σA has been crystallized in space group P1, with unit-cell parameters a = 103.2, b = 129.9, c = 144.0 Å, α = 93.8, β = 105.1, γ = 98.2°. A complete data set has been collected to 2.3 Å resolution and analyzed.

Crystallization and preliminary X-ray diffraction studies of hexokinase KlHxk1 from Kluyveromyces lactis

Crystals of the enzyme hexokinase 1 from the yeast K. lactis (KlHxk1) suitable for X-ray analysis were obtained in various space groups.

Crystallization of a Mos1 transposase–inverted-repeat DNA complex: biochemical and preliminary crystallographic analyses

Full-length Mos1 transposase has been crystallized in a complex with inverted-repeat DNA. Crystals diffract to 3.25 Å resolution and display noncrystallographic twofold symmetry.

The crystallization of apo-form UMP kinase from Xanthomonas campestris is significantly improved in a strong magnetic field

A bacterial UMP kinase from the plant pathogen X. campestris pathovar campestris has been overexpressed in E. coli, purified and crystallized in a strong magnetic field. The crystals diffracted to 2.35 Å.

Crystallization and preliminary X-ray analysis of Atg10

S. cerevisiae Atg10, an E2-like enzyme that mediates the conjugation reaction between Atg12 and Atg5, was crystallized and diffracted to 2.3 Å resolution.

Preliminary X-ray crystallographic study of glucose de­hydrogenase from Thermus thermophilus HB8

The glucose dehydrogenase (GDH) protein from T. thermophilus HB8 was cloned, expressed, purified and crystallized. GDH crystals belong to space group P2₁ and diffract to 1.9 Å resolution.

Expression, purification and preliminary crystallographic characterization of FlhF from Bacillus subtilis

Preliminary crystallographic data are reported for the third SRP GTPase FlhF from Bacillus subtilis.

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase

As part of work on S. aureus, the crystallization of Sar2028, a protein that is upregulated in MRSA, is reported.