Validation of macromolecular structures: updating standards for publication of NMR structures in an IUCr journal

Structural basis for the binding of naproxen to human serum albumin in the presence of fatty acids and the GA module

The crystal structure of the complex formed by human serum albumin and a bacterial albumin-binding domain with the drug compound naproxen and fatty acid decanoate bound to albumin is described.

Structure of calcium-bound human S100A13 at pH 7.5 at 1.8 Å resolution

The crystal structure of Ca²⁺-bound human S100A13 at pH 7.5 is reported.

Preliminary X-ray characterization of a novel type of anchoring cohesin from the cellulosome of Ruminococcus flavefaciens

The cloning, expression, purification, crystallization and preliminary X-ray characterization of a novel class of cohesin module (type III) from the R. flavefaciens ScaE anchoring scaffoldin are described.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the mannose 6-phosphate isomerase from Salmonella typhimurium

The cloning, expression, purification, crystallization and preliminary X-ray crystallographic studies of mannose 6-phosphate isomerase from S. typhimurium are reported.

Crystallization and preliminary X-ray diffraction analysis of a gluta­thione S-transferase from Xylella fastidiosa

Glutathione S-transferase from X. fastidiosa (xfGST) has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.23 Å.

Purification, crystallization and preliminary crystallographic study of a recombinant plant amino­aldehyde de­hydrogenase from Pisum sativum

A 2.8 Å resolution data set was collected from a crystal of a recombinant plant aminoaldehyde dehydrogenase from P. sativum. This enzyme oxidizes ω-aminoaldehydes arising from polyamine degradation.

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

A non-Pfam protein, AF1514, from A. fulgidus has been crystallized.

Crystallization and preliminary diffraction analysis of a DsbA homologue from Wolbachia pipientis

The first crystallization of a W. pipientis protein, α-DsbA1, was achieved using hanging-drop and sitting-drop vapour diffusion.

Purification, crystallization and preliminary X-ray analysis of inositol de­hydrogenase (IDH) from Bacillus subtilis

Selenomethionine-substituted IDH was crystallized using the microbatch method. The crystals diffracted to beyond 2.0 Å resolution using synchrotron radiation.

Crystallization and preliminary crystallographic studies of L30e, a ribosomal protein from Methanocaldococcus jannaschii (MJ1044)

The ribosomal protein (L30e) from M. jannaschii was cloned from the gene MJ1044, expressed, purified and crystallized. The crystal belongs to the primitive tetragonal space group P4₃ and diffracted to 1.9 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of a 6-pyruvoyltetra­hydropterin synthase homologue from Esherichia coli

The 6-pyruvoyltetrahydropterin synthase from E. coli has been crystallized in two crystal forms. Diffraction data were collected from hexagonal- and rectangular-shaped crystals to 3.0 and 2.3 Å, respectively.

Preliminary X-ray crystallographic studies of mouse UPR responsive protein P58(IPK) TPR fragment

To investigate the mechanism by which P58(IPK) functions to promote protein folding within the ER, a P58(IPK) TPR fragment without the C-terminal J-domain has been crystallized.

Preliminary crystallographic data of the three homologues of the thiol–di­sulfide oxidoreductase DsbA in Neisseria meningitidis

The Neisseria meningitidis genome possesses three genes encoding active DsbAs. To throw light on the reason for this genetic multiplicity, the three enzymes have been purified and crystallized.

Overproduction, purification and preliminary crystallographic analysis of the carbohydrate-recognition domain of human langerin

Crystals of the carbohydrate-recognition domain of human langerin were obtained that diffracted synchrotron radiation to 1.5 Å resolution.

Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis

B. subtilis queD was overexpressed, purified and crystallized. A diffraction data set was collected to a resolution of 3.6 Å.

Crystallization of the extracellular rubber oxygenase RoxA from Xanthomonas sp. strain 35Y

The extracellular rubber-degrading enzyme rubber oxygenase A (RoxA) from Xanthomonas sp. strain 35Y has been crystallized and diffraction data have been collected to high resolution.

Purification, crystallization and preliminary X-ray analysis of aminoglycoside-2′′-phosphotransferase-Ic [APH(2′′)-Ic] from Enterococcus gallinarum

APH(2′′)-Ic is an enzyme that is responsible for high-level gentamicin resistance in E. gallinarum isolates. Crystals of the wild-type enzyme and three mutants have been prepared and a complete X-ray diffraction data set was collected to 2.15 Å resolution from an F108L crystal.

Crystallization and preliminary X-ray crystallographic analyses of Nur, a nickel-responsive transcription regulator from Streptomyces coelicolor

A nickel uptake regulator (Nur) from Streptomyces coelicolor has been crystallized. A 2.4 Å native data set and a 3.0 Å Ni-MAD data set were collected using synchrotron radiation.

Crystallization and preliminary crystallographic analysis of human Ca²⁺-loaded calbindin-D28k

Human calbindin-D28k has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.

Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis

Three mutants of the haloalkane dehalogenase DhaA derived from R. rhodochrous NCIMB 13064 were crystallized and diffracted to ultrahigh resolution.

Purification, crystallization and preliminary crystallographic analysis of Streptococcus pyogenes laminin-binding protein Lbp

The S. pyogenes laminin-binding protein Lbp, which is essential for adhesion to human laminin, has been expressed, purified and crystallized.

Purification, crystallization and preliminary structural characterization of the periplasmic domain P1 of the Escherichia coli membrane-protein insertase YidC

The overproduction, purification, crystallization and preliminary crystallographic studies of the native and selenomethionine-labelled P1 domain are reported here as a first step towards the elucidation of the molecular mechanism of YidC as a membrane-protein insertase.

Notes for authors 2008