Structure of the twin-arginine signal-binding protein DmsD from Escherichia coli

The crystal structure of DmsD from E. coli has been determined at 2.4 Å resolution.

Structure and biochemical characterization of an adenylate kinase originating from the psychrophilic organism Marinibacillus marinus

The crystal structure of adenylate kinase from the psychrophile M. marinus has been determined at 2.0 Å resolution and the kinetic parameters of this cold-adapted enzyme have been examined.

Structure of the choline-binding domain of Spr1274 in Streptococcus pneumoniae

The crystal structure of the choline-binding domain of Spr1274 (residues 44–129) has been solved at 2.38 Å resolution with three molecules in the asymmetric unit.

The Z isomer of 2,4-di­aminofuro[2,3-d]pyrimidine antifolate promotes unusual crystal packing in a human di­hydrofolate reductase ternary complex

The crystal structure of the Z-isomer of 2,4-diamino-5-[2-(2′-methoxyphenyl)-propenyl]-furo[2,3-d]pyrimidine shows an unusual packing arrangement in which the conserved active site Arg70 forms a salt bridge to the side chain of Glu44 from a symmetry-related molecule.

Crystallization and preliminary diffraction analysis of a β-galactosidase from Trichoderma reesei

The crystallization and preliminary diffraction analysis of a β-galactosidase from T. reesei is described. The crystals diffracted to 1.2 Å resolution.

Crystallization and preliminary crystallographic analysis of the bacterial capsule assembly-regulating tyrosine phosphatases Wzb of Escherichia coli and Cps4B of Streptococcus pneumoniae

The crystallization is reported of two bacterial tyrosine phosphatases which belong to different enzyme families despite their ability to catalyse identical reactions.

Purification, crystallization and preliminary crystallographic study of haemoglobin from camel (Camelus dromedarius): a high oxygen-affinity lowland species

Haemoglobin from Camelus dromedarius provides an interesting case study of adaptation to life in deserts at extremely high temperatures. An ambition to unravel the integrated structural and functional aspects of the casual survival of this animal at high temperatures led the authors to specifically work on this problem. This work reports the preliminary crystallographic study of camel haemoglobin.

Crystallization and preliminary X-ray characterization of a PaaX-like protein from Sulfolobus solfataricus P2

In this study, the PaaX-like protein from the hyperthermophilic archaeon Sulfolobus solfataricus P2 was successfully crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant.

Purification, crystallization and preliminary data analysis of ligand–receptor complexes of growth and differentiation factor 5 (GDF5) and BMP receptor IB (BRIB)

Crystallization of the ligand–receptor complex of GDF5 and its type I receptor BRIB requires the presence of the activin type IIB receptor (ActRIIB) for crystal formation, but ActRIIB is not part of the complex found in the asymmetric unit.

Crystallization and preliminary X-ray diffraction analysis of the truncated cytosolic domain of the iron transporter FeoB

The truncated cytosolic domain of the iron transporter FeoB from T. maritima was overexpressed, purified and crystallized. Four native or SeMet crystal forms in a nucleotide-free state or in complex with either GDP or GMPPNP diffracted to resolutions of between 1.5 and 2.1 Å.

Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus

The N-terminal domain of nsp2 from avian infectious bronchitis virus has been purified and crystallized. The crystals diffracted to 2.5 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of the DNA-binding domain of a Chlamydia trachomatis OmpR/PhoB-subfamily response regulator homolog, ChxR

Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the DNA-binding effector domain from an atypical OmpR response regulator homolog, ChxR, encoded by Chlamydia trachomatis are reported.

Cloning, purification, crystallization and preliminary X-ray studies of flagellar hook scaffolding protein FlgD from Pseudomonas aeruginosa PAO1

In order to better elucidate the functions of FlgD in flagellar hook biosynthesis, the three-dimensional structure of FlgD is being determined by X-­ray crystallo­graphy. Here, the expression, purification, crystallization and preliminary crystallographic analysis of FlgD from P. aeruginosa are reported.

Complete amino-acid sequence, crystallization and preliminary X-ray diffraction studies of leucurolysin-a, a nonhaemorrhagic metalloproteinase from Bothrops leucurus snake venom

Leucurolysin-a, a nonhaemorrhagic metalloproteinase from B. leucurus snake venom, has been crystallized in a free form and in a complexed form.

Expression, purification, crystallization and preliminary crystallographic analysis of SpaA, a major pilin from Corynebacterium diphtheriae

SpaA, one of the major pilins of C. diphtheriae, has been expressed, purified and crystallized and X-ray diffraction data have been collected to 1.6 Å resolution.

Preliminary X-ray analysis of twinned crystals of sarcosine di­methylglycine methyltransferase from Halorhodospira halochoris

The crystallization and preliminary X-ray diffraction analysis of sarcosine dimethylglycine methyltransferase from H. halochoris is reported.

Crystallization and X-ray diffraction analysis of an `all-locked' nucleic acid duplex derived from a tRNA^Ser microhelix

A completely `all-locked' nucleic acid duplex was designed from an E. coli tRNA^Ser microhelix. The helix consists exclusively of LNA building blocks and was crystallized. The crystals diffracted to 1.9 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homologue of γ-AMPK

A preliminary crystallographic analysis at 2.3 Å resolution of protein MJ1225 from M. jannaschii, a putative archaeal homolog of γ-AMPK, is described.

Crystallization and preliminary X-ray diffraction of chlorite dismutase from De­chloromonas aromatica RCB

Chlorite dismutase crystals belonged to space group P2₁2₁2₁ and diffracted to 3.0 Å resolution at a third-generation synchrotron X-ray source.

Crystallization and preliminary X-ray analysis of γ-hexa­chloro­cyclo­hexane de­hydrochlorinase LinA from Sphingobium japonicum UT26

Purification and crystallization of LinA allowed the collection of data to 2.25 Å resolution.

Purification, crystallization and preliminary X-ray analysis of FliT, a bacterial flagellar substrate-specific export chaperone

FliT is a cytoplasmic flagellar type III substrate-specific export chaperone; it has been expressed, purified and crystallized and the crystals have been characterized by X-ray diffraction.

Crystallization and preliminary X-ray analysis of AAMS amido­hydrolase, the final enzyme in degradation pathway I of pyridoxine

Recombinant α-(N-acetylaminomethylene)succinic acid amidohydrolase from M. loti MAFF303099 was crystallized and diffraction data were collected at 2.7 Å resolution.

Crystallization and preliminary X-ray diffraction of the DEAD-box protein Mss116p complexed with an RNA oligonucleotide and AMP-PNP

The DEAD-box protein Mss116p was crystallized in a complex with a U₁₀ RNA oligonucleotide and an ATP analog. High glycerol (50%) and L-­arginine plus L-glutamate (50 mM each) enabled the concentration of Mss116p to ∼10 mg ml⁻¹ for crystallization.

Crystallization and preliminary X-ray analysis of eukaryotic initiation factor 4E from Pisum sativum

Crystals of N-terminally truncated eIF4E from pea were obtained and X-ray data were recorded in-house to a resolution of 2.2 Å.

Preliminary X-ray crystallographic analysis of sulfide:quinone oxidoreductase from Acidi­thiobacillus ferrooxidans

The sulfide:quinone oxidoreductase from A. ferrooxidans ATCC 23270 was overexpressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis were performed for the recombinant enzyme.

Expression, purification and preliminary X-ray crystallographic analysis of UDP-galacto­pyranose mutase from Deinococcus radiodurans

UDP-galactopyranose mutase was crystallized using the microbatch method. The crystals diffracted to 2.36 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray analysis of a complex formed between the antibiotic simocyclinone D8 and the DNA breakage–reunion domain of Escherichia coli DNA gyrase

Crystals of a complex formed between the 59 kDa N-terminal fragment of the E. coli DNA gyrase A subunit and the antibiotic simocyclinone D8 were obtained and X-ray data were recorded to a resolution of 2.75 Å.

Purification, crystallization and preliminary X-ray crystallographic studies of the complex between Smc5 and the SUMO E3 ligase Mms21

The Mms21–Smc5 protein complex has been crystallized. The diffraction of the crystals was improved greatly by glutaraldehyde treatment and X-ray diffraction data sets were collected to resolutions of 2.3 and 3.9 Å from native and selenomethionine-derivative protein crystals, respectively.

Crystallization and preliminary X-ray analysis of the LOV domain of the blue-light receptor YtvA from Bacillus amyloliquefaciens FZB42

The crystallization of the LOV domain of the blue-light receptor YtvA from B. amyloliquefaciens FZB42 is described. The crystals diffracted to 1.60 Å resolution.

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas. Corrigendum

A corrigendum to the article by Najmudin et al. [Acta Cryst. (2009). F65, 730–732].