The structure of phosphate-bound Escherichia coli adenylosuccinate lyase identifies His171 as a catalytic acid

Here, the crystal structure of adenylosuccinate lyase from Escherichia coli was determined to 1.9 Å resolution.

Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by mono­ethylphosphonate

The crystal structure of the esterase EstA from a cold-adapted bacterium was determined in a form that was covalently inhibited by monoethylphosphonate.

Structure of Escherichia coli malate de­hydrogenase at 1.45 Å resolution

The structure of apo malate dehydrogenase from Escherichia coli has been determined to 1.45 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of the N-terminal domain of GNBP3 from Drosophila melanogaster

Crystals of the N-terminal domain of Gram-negative bacteria-binding protein 3 of D. melanogaster grown from PEG solutions are monoclinic (space group C2) and diffract to 1.7 Å resolution.

Crystallization and preliminary X-ray analysis of βC–S lyases from two oral streptococci

The βC-S lyases from two oral bacteria, Streptococcus anginosus and S. gordonii, were cloned, overproduced, purified and crystallized. The obtained crystals were characterized by X-ray diffraction.

Crystallization and preliminary X-ray analysis of the small subunit (R2F) of native ribonucleotide reductase from Corynebacterium ammoniagenes

The crystallization of the metallo-cofactor (R2F) of native ribonucleotide reductase isolated from the Mn-requiring Gram-positive bacterium C. ammoniagenes is described. The crystals diffracted to 1.36 Å resolution.

Crystallization and preliminary X-ray diffraction data of an LNA 7-mer duplex derived from a ricin aptamer

An all-LNA duplex was designed from the stem region of an RNA aptamer which has been generated against ricin. The LNA duplex was crystallized and preliminary X-ray diffraction analysis revealed diffraction to a resolution of up to 2.8 Å.

Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti

Recombinant 4-pyridoxolactonase from M. loti MAFF303099 was crystallized in two forms and diffraction data were collected to 2.0 and 1.9 Å resolution, respectively.

Crystallization and preliminary X-ray diffraction analysis of motif N from Saccharomyces cerevisiae Dbf4

To understand the role of the Cdc7–Dbf4 complex in checkpoint responses, a fragment of Saccharomyces cerevisiae Dbf4 encom­passing motif N was isolated, overproduced and crystallized.

Crystallization and preliminary structural studies of champedak galactose-binding lectin

Galactose-binding lectin from champedak was crystallized at 293 K. Preliminary X-ray diffraction analyses are reported.

Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231

The crystallization of a novel controller protein is reported and its interaction with DNA is characterized.

Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-α-L-arabinanase from hyperthermophilic Thermotoga petrophila

The crystallization and preliminary X-ray diffraction studies of an endo-1,5-α-arabinanase from hyperthermophilic T. petrophila are reported. The crystals diffracted to 2.86 Å resolution.

Expression, purification and preliminary X-ray analysis of proliferating cell nuclear antigen from the archaeon Thermococcus thioreducens

The proliferating cell nuclear antigen (PCNA) from a novel hyperthermophilic archaeon Thermococcus thioreducens has been crystallized, and diffraction data have been collected to 1.86 Å.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of Mycoplasma genitalium protein MG289

Single orthorhombic crystals of M. genitalium protein MG289 have been grown and shown to diffract X-rays to 2.8 Å resolution with good statistics. The structure obtained from these data will help to provide insight into the function of the protein as well as improving the understanding of its role in this human pathogen.

Crystallization and preliminary crystallographic analysis of poly-γ-glutamate hydrolase from bacteriophage ΦNIT1

Poly-γ-glutamate hydrolase from bacteriophage ΦNIT1 was crystallized by the sitting-drop vapour-diffusion method and the crystals diffracted to beyond 2.4 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of PhoK, an extracellular alkaline phosphatase from Sphingomonas sp. BSAR-1

A new alkaline phosphatase enzyme from Sphingomonas sp. strain BSAR-1, termed PhoK, has been shown to be useful in uranium bioprecipitation. PhoK has been expressed, purified and crystallized.

Purification, crystallization and preliminary crystallographic analysis of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough

Crystals of the soluble form of the [NiFeSe] hydrogenase from D. vulgaris Hildenborough were obtained and belonged to the monoclinic space group P2₁, with unit-cell parameters a = 60.57, b = 91.05, c = 66.85 Å, β = 101.46°.

Crystallization and preliminary X-ray analysis of flap endonuclease 1 (FEN1) from Desulfurococcus amylolyticus

Flap endonuclease 1 from D. amylolyticus was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 2.00 Å resolution.

Cobalt-, zinc- and iron-bound forms of adenylate kinase (AK) from the sulfate-reducing bacterium Desulfovibrio gigas: purification, crystallization and preliminary X-ray diffraction analysis

Adenylate kinase (AK) from D. gigas was purified and crystallized in three different metal-bound forms: Zn²⁺–AK, Co²⁺–AK and Fe²⁺–AK.

Crystallization and preliminary X-ray diffraction studies of the ubiquitin-like (UbL) domain of the human homologue A of Rad23 (hHR23A) protein

The ubiquitin-like domain of human hHR23A protein was crystallized by the hanging-drop vapour-diffusion method in space group P6₅22 and diffraction data were collected to 1.97 Å resolution. Structure solution by molecular replacement is described.

Crystallization and preliminary X-ray analysis of aspartate transcarbamoylase from the parasitic protist Trypanosoma cruzi

Aspartate transcarbamoylase, the second enzyme of the de novo pyrimidine-biosynthetic pathway, from T. cruzi has been purified and crystallized for X-ray structure analysis.

Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate de­hydrogenase from Antheraea mylitta

The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of glyceraldehyde-3-phosphate dehydrogenase from A. mylitta are reported.

Crystallization of mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode Ascaris suum with the specific inhibitor flutolanil

Rhodoquinol-fumarate reductase is a key enzyme in the anaerobic respiratory chain of adult A. suum mitochondria. Its crystallization in the presence of a mixture of octaethyleneglycol monododecyl ether and n-dodecyl-β-D-maltopyranoside in a form suitable for X-ray structure analysis is reported.

Crystallization and preliminary X-ray analysis of the complexes between a Fab and two forms of human insulin-like growth factor II

Complexes of both hIGF-II and hIGF-IIE with a Fab have been crystallized and investigated by X-ray analysis.

Purification, crystallization and preliminary X-ray analysis of urease from jack bean (Canavalia ensiformis)

Jack bean urease was purified and crystallized and X-ray diffraction data were collected to 2.05 Å resolution.

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of leucine aminopeptidase (LAP) from the pepA gene of Xanthomonas oryzae pv. oryzae

Leucine aminopeptidase, an exopeptidase that hydrolyzes leucine from the N-terminus of polypeptides, from X. oryzae pv. oryzae was cloned, expressed and crystallized.

Crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of the human spliceosomal DExD/H-box protein hPrp22

The cloning, purification and crystallization of the C-terminal domain of human hPrp22 are reported. This communication also contains data for the preliminary X-ray diffraction analysis.

Purification, crystallization and initial X-ray diffraction study of the zinc-finger domain of zebrafish Nanos

Crystallization of Nanos.