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Structure of bovine pancreatic ribonuclease complexed with uridine 5′-monophosphate at 1.60 Å resolution

The crystal structure of bovine pancreatic ribonuclease A in complex with uridine 5′-monophosphate was determined at 1.60 Å resolution. The uridine lies in the pyrimidine-specific binding site of the enzyme, with the phosphate group extended into the solvent without interacting with the protein.

Structure of Bacillus amyloliquefaciens α-amylase at high resolution: implications for thermal stability

The crystal structure of B. amyloliquefaciens α-amylase (BAA) at 1.4 Å resolution revealed ambiguities in the thermal adaptation of homologous proteins in this family.

Structure of uracil-DNA N-glycosylase (UNG) from Vibrio cholerae: mapping temperature adaptation through structural and mutational analysis

The crystal structure of uracil-DNA N-glycosylase from Vibrio cholerae has been determined at 1.5 Å, and used for mapping temperature adaptation.

A new crystal form of human di­amine oxidase

The structure of human diamine oxidase has been refined to 2.1 Å resolution in space group C222₁.

A conserved mechanism of autoinhibition for the AMPK kinase domain: ATP-binding site and catalytic loop refolding as a means of regulation

A 1.9 Å resolution crystal structure of the isolated kinase domain from the α2 subunit of human AMPK, the first from a multicellular organism, is presented.

A micromolar O-sulfated thio­hydroximate inhibitor bound to plant myrosinase

The crystal structure of an O-sulfated thiohydroximate inhibitor bound to S. alba myrosinase is reported.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the collagen-binding region of RspB from Erysipelothrix rhusiopa­thiae

The expression, purification and crystallization of the collagen-binding region of the E. rhusiopathiae surface protein RspB is described. The crystals diffracted to 2.2 Å resolution using synchrotron radiation.

A preliminary X-ray study of D,D-heptose-1,7-bis­phosphate phosphatase from Burkholderia thailandensis E264

In this study, D,D-heptose-1,7-bisphosphate phosphatase has been cloned, expressed, purified and crystallized.

Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum

Recombinant P. somniferum salutaridine reductase (SalR) was purified and crystallized with NADPH using the hanging-drop vapor-diffusion method. Crystals of the SalR–NADPH complex diffracted X-rays to a resolution of 1.9 Å.

Purification and crystallization of Phd, the antitoxin of the phd/doc operon

The antitoxin Phd from the phd/doc operon of bacteriophage P1 was crystallized in two distinct crystal forms.

Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus

The purification, crystallization and preliminary X-ray diffraction analysis of a surface-associated antigen from the major human hookworm N. americanus is presented.

Purification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes

The minor pilin FctB from S. pyogenes strain 90/306S was expressed in E. coli, purified and crystallized. The hexagonal FctB crystals diffracted to 2.9 Å resolution.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3

SAICAR synthetase from P. horikoshii OT3 has been cloned, expressed, purified and crystallized.

Expression, purification and X-ray analysis of 1,3-propanediol de­hydrogenase (Aq_1145) from Aquifex aeolicus VF5

1,3-Propanediol dehydrogenase (Aq_1145) from A. aeolicus VF5 has been overexpressed, purified and crystallized. The crystals diffracted to 2.4 Å resolution.

Crystallization and preliminary X-ray diffraction analyses of the homodimeric glycine de­carboxylase (P-protein) from the cyanobacterium Synechocystis sp. PCC 6803

Recombinant glycine decarboxylase from Synechocystis sp. PCC 6803 was expressed in E. coli and purified to homogeneity. Crystals were obtained that diffracted to 2.1 Å resolution using synchrotron radiation.

Expression, purification and preliminary crystallographic analysis of recombinant human DEAD-box polypeptide 5

The production and crystallization of the human DEAD box polypeptide 5 are reported. A 2.7 Å native diffraction data set has been obtained.

Preliminary crystallographic characterization of the Grb2 SH2 domain in complex with a FAK-derived phosphotyrosyl peptide

Crystals of the Grb2 SH2 domain in complex with a phosphotyrosyl peptide corresponding to residues 921–930 of focal adhesion kinase (FAK) have been obtained using the sitting-drop vapour-diffusion technique. Data have been collected to 2.49 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of a highly specific serpin from the beetle Tenebrio molitor

In this study, the mature form of SPN48 was overexpressed in Escherichia coli and purified. The purified SPN48 protein was crystallized using 14% polyethylene glycol 8000 and 0.1 M 2-(N-morpho­lino)ethanesulfonic acid pH 6.0 as the precipitant. The crystals diffracted X-rays to 2.1 Å resolution and were suitable for structure determination.

Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana

VSP1 from Arabidopsis thaliana was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to 1.9 Å resolution.

Crystallization and preliminary X-ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho

Psu, a unique 21 kDa protein from bacteriophage P4, is a non-essential capsid-decoration protein that inhibits Rho-dependent termination specifically and efficiently both in vivo and in vitro. Psu has been crystallized using PEG as precipitant and the crystals diffracted to 2.3 Å resolution.

Crystallization and preliminary crystallographic analysis of eukaryotic transcription and mRNA export factor Iws1 from Encephalitozoon cuniculi

E. cuniculi Iws1, which acts in eukaryotic transcription and mRNA processing and export, has been crystallized and data have been collected to 2.5 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of human FAF1 UBX domain

The FAF1 UBX domain was crystallized. X-ray diffraction data were collected to 3.00 Å resolution and the crystals belonged to space group F4₁32.

Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1. Addendum

Addendum to Hee et al. [Acta Cryst. (2009), F65, 422–425].