An improved strategy for the crystallization of Leishmania mexicana pyruvate kinase

The first crystal structure of Leishmania mexicana pyruvate kinase (LmPYK) obtained at a neutral pH. LmPYK was co-crystallized with the small molecule 1,3,6,8-pyrenetetrasulfonic acid, which provides a helpful intermolecular bridge between macromolecules.

The structure of DinB from Geobacillus stearothermophilus: a representative of a unique four-helix-bundle superfamily

The structure of DinB from G. stearothermophilus is described and compared with a number of recently reported structures of this unusual fold. Structural similarities are revealed that unite several distant protein families.

Revisiting glutaraldehyde cross-linking: the case of the Arg–Lys intermolecular doublet

A specific Lys–Arg cross-link is observed in trimeric barnase crystals.

Noncanonical conformation of CDR L1 in the anti-IL-23 antibody CNTO4088

The X-ray structure of the anti-IL-23 antibody CNTO4088 reveals an unusual conformation of CDR L1 despite the fact that the residues in key positions fit the pattern of the canonical structure.

Structural implications of a G170R mutation of alanine:glyoxylate aminotransferase that is associated with peroxisome-to-mitochondrion mistargeting

The crystal structure of the G170R mutant form of human alanine:glyoxylate aminotransferase has been determined at 2.6 Å resolution. This mutation is associated with enzyme mistargeting in the hereditary kidney-stone disease primary hyperoxaluria type 1.

A triclinic crystal form of Escherichia coli 4-di­phosphocytidyl-2C-methyl-D-erythritol kinase and reassessment of the quaternary structure

The structure of a triclinic crystal form of 4-diphosphocytidyl-2C-methyl-D-erythritol kinase has been determined. Comparisons with a previously reported monoclinic crystal form raise questions about our knowledge of the quaternary structure of this enzyme.

Apo and ligand-bound structures of ModA from the archaeon Methanosarcina acetivorans

Crystal structures of ModA from M. acetivorans in the apo and ligand-bound conformations confirm domain rotation upon ligand binding.

X-ray structure of the NO-bound Cu_B in bovine cytochrome c oxidase

The structure of nitric oxide-treated bovine heart cytochrome c oxidase (CcO) in the fully reduced state was determined at 50 K under light illumination.

Structure of native laccase B from Trametes sp. AH28-2

The crystal structure of a fully active laccase from Trametes sp. AH28-2 is reported.

X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid

Crystals of insulin have been grown from a solution containing insulin and polysialic acid and the three-dimensional structure of insulin in these crystals has been solved at 1.6 Å resolution.

Two-step counterdiffusion protocol for the crystallization of haemoglobin II from Lucina pectinata in the pH range 4–9

oxyHbII crystals have been grown at pH 4, 5, 8 and 9 by capillary counterdiffusion technique by a two-step protocol: (i) mini screen (searching step) and (ii) pH screen (optimization step).

Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose de­hydrogenase from Burkholderia cepacia IST408

Crystals of B. cepacia IST408 UDP-glucose dehydrogenase (EC 1.1.1.22) were obtained in space group P2₁2₁2₁ and diffracted to 2.09 Å resolution.

Crystallization and preliminary X-ray analysis of the MaoC-like dehydratase from Phytophthora capsici

The MaoC-like dehydratase from P. capsici was cloned, expressed and purified to homogeneity. Crystals were obtained that diffracted to 1.93 Å resolution.

Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei

Cyanide-insensitive alternative oxidase from T. brucei brucei has been purified and crystallized for X-ray structure analysis.

Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P¹,P⁴-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

A novel diadenosine 5′,5′′′-P¹,P⁴-tetraphosphate phosphorylase from M. tuberculosis H37Rv has been crystallized. The crystal belonged to space group C2 and diffracted X-rays to 1.9 Å resolution.

Crystallization and preliminary crystallographic analysis of maganese(II)-dependent 2,3-di­hydroxybi­phenyl 1,2-dioxygenase from Bacillus sp. JF8

A thermostable manganese(II)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from Bacillus sp. JF8 was crystallized in two forms using the sitting-drop vapour-diffusion method. Both crystals diffracted to approximately 1.3 Å resolution.

Purification, crystallization and preliminary X-ray studies of the putative lysozyme SP0987 from Streptococcus pneumoniae

Here, the expression, purification, crystallization and preliminary crystallographic analysis of SP0987 from Streptococcus pneumoniae TIGR4 are reported.

Crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen Dichelobacter nodosus

The subtilisin-like proteases AprV2 and BprV from virulent strains and AprB2 and BprB from benign strains of D. nodosus have been crystallized and X-ray diffraction data have been collected to 2.0, 2.0, 1.7 and 1.8 Å resolution, respectively.

Crystallization of community-acquired respiratory distress syndrome toxin from Mycoplasma pneumoniae

Community-acquired respiratory distress syndrome toxin (CARDS TX) from M. pneumoniae was cloned, expressed, purified and crystallized. A diffraction data set from a native CARDS TX crystal was obtained at 2.2 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of β-galactosidase from Kluyveromyces lactis

β-Galactosidase from K. lactis has been expressed in S. cerevisiae, purified by affinity chromatography and crystallized in its native form.

Crystallization and preliminary X-ray studies of ferredoxin-NADP⁺ oxidoreductase encoded by Bacillus subtilis yumC

Ferredoxin-NADP⁺ oxidoreductase from B. subtilis has been crystallized by the sitting-drop vapour-diffusion method. Diffraction data were collected to 1.8 Å resolution.

Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase

Glycerol kinase from human African trypanosomes has been purified and crystallized for X-ray structure analysis.

Crystallization and preliminary X-ray crystallographic studies of an exo-β-D-glucosaminidase from Trichoderma reesei

An exo-β-D-glucosaminidase from T. reesei (Gls93) has been crystallized by the hanging-drop vapour-diffusion method. Diffraction data have been collected using synchrotron radiation.

Crystallization of mouse S-adenosyl-L-homocysteine hydrolase

Mouse S-adenosyl-L-homocysteine hydrolase has been crystallized in the presence of the reaction product adenosine. Diffraction data to 1.55 Å resolution were collected using synchrotron radiation.

Purification, crystallization and X-ray crystallographic analysis of Archaeoglobus fulgidus neelaredoxin

Diffraction-quality crystals of A. fulgidus neelaredoxin have been produced. The expression, purification, crystallization and X-ray crystallographic analysis of A. fulgidus neelaredoxin are reported.

Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 β-glucosidase

Recombinant rice Os4BGlu12 β-glucosidase purified from E. coli was crystallized with and without 2,4-dinitrophenyl-2-deoxy-2-fluoro-β-D-glucopyranoside.

Purification and crystallization of the entire recombinant subunit E of the energy producer A₁A_o ATP synthase

The full-length subunit E of the ATP synthase from P. horikoshii OT3 was cloned, overexpressed, purified and crystallized. The crystals belonged to space group I4 and diffracted to 3.3 Å resolution using synchrotron radiation.

Purification and crystallization of a multimodular heterotrimeric complex containing both type I and type II cohesin–dockerin interactions from the cellulosome of Clostridium thermocellum

A multimodular heterotrimeric complex encompassing a trimodular C-terminal fragment of the cellulosomal scaffoldin CipA from C. thermocellum bound to the type II cohesin module of SdbA and the type I dockerin module of CelD has been crystallized by the hanging-drop vapour-diffusion method and initial X-ray diffraction data analysis has been conducted.

Crystallization and preliminary X-ray crystallographic study of 1-de­oxy-D-xylulose 5-phosphate reductoisomerase from Plasmodium falciparum

1-Deoxy-D-xylulose 5-phosphate reductoisomerase from P. falciparum has been crystallized in the presence of NADPH. Diffraction data to 1.85 Å resolution have been collected using synchrotron radiation.

Crystallization and preliminary X-ray crystallographic study of phosphoglucose isomerase from Plasmodium falciparum

Phosphoglucose isomerase from P. falciparum has been crystallized. Diffraction data to 1.8 Å resolution have been collected using synchrotron radiation.

Purification, crystallization and preliminary data analysis of FocB, a transcription factor regulating fimbrial adhesin expression in uropathogenic Escherichia coli

The focB gene from E. coli was cloned and the protein was expressed and purified. The protein is oligomeric, most likely in the form of dimers. NMR analysis showed that line broadening significantly increases at temperatures below 293 K, presumably owing to increased conformational exchange or oligomerization. Crystals were obtained at room temperature and diffracted to better than 2.0 Å resolution.

Crystallization and preliminary crystallographic studies of the catalytic subunits of human pyruvate de­hydrogenase phosphatase isoforms 1 and 2

The catalytic subunits of the human pyruvate dehydrogenase phosphatase isoforms 1 and 2 have been purified and crystallized. The crystals of PDP1c and PDP2c diffracted to 2.45 and 2.0 Å resolution, respectively.

Crystallization and X-ray diffraction studies of cellobiose phosphorylase from Cellulomonas uda

Preliminary crystallographic studies of recombinant cellobiose phosphorylase from Cellulomonas uda in complex with reaction substrates and products have led to high-quality diffraction data at high resolution, thus enabling structural studies to dissect the structural and mechanistic determinants of disaccharide phosphorylase activity.

Isolation and purification of Thermus thermophilus HpaB by a crystallization approach

Microcrystals of HpaB were formed while isolating integral membrane respiratory complexes from Thermus thermophilus. Further purification of the enzyme was achieved by repetitive crystallization. Subsequently, well shaped single crystals of the native enzyme that diffracted to 1.82 Å resolution were grown in sitting drops.

Crystallization and preliminary crystallographic analysis of mouse peroxiredoxin II with significant pseudosymmetry

Mouse peroxiredoxin II was crystallized in an orthorhombic space group and native X-ray diffraction data were collected.

Expression, purification, crystallization and preliminary X-ray analysis of calmodulin in complex with the regulatory domain of the plasma-membrane Ca²⁺-ATPase ACA8

Plant plasma-membrane Ca²⁺-ATPase is regulated via binding of calmodulin to its autoinhibitory N-terminal domain. In this study, the expression, purification, crystallization and preliminary X-ray diffraction analysis of this protein complex from A. thaliana are reported.

Evaluating the efficacy of tryptophan fluorescence and absorbance as a selection tool for identifying protein crystals

The effectiveness of using ultraviolet microscopes to illuminate protein crystals in high throughput screens is evaluated.