Challenging semi-bootstrapping molecular-replacement strategy reveals intriguing crystal packing of rhizavidin

The structure of the rhizavidin–biotin complex was determined via combined molecular replacement approaches. Both the apo and biotin complexed structures pack in unique oligomeric states in the corresponding crystals.

X-ray structure of perdeuterated diiso­propyl fluorophosphatase (DFPase): perdeuteration of proteins for neutron diffraction

The crystal structure of perdeuterated diisopropyl fluorophosphatase is reported and compared with the hydrogenated structure. Diffraction guidelines for neutron crystallography experiments are summarized.

X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia

The three-dimensional structure of carbamate kinase from the human parasite G. lamblia has been determined at 3 Å resolution. Steady-state kinetic parameters have been determined and dsRNA-silencing experiments indicate that the enzyme is essential for the survival of G. lamblia.

Structure of the aliphatic sulfonate-binding protein SsuA from Escherichia coli

Sulfur is an essential component for the biosynthesis of the sulfur-containing amino acids L-methionine and L-cysteine. The crystal structure of the periplasmic aliphatic sulfonate-binding protein SsuA from E. coli has been solved at 1.75 Å resolution in a substrate-free state. Comparison with the substrate-bound protein shows significant movement of one of the two domains.

Structure of ST0929, a putative glycosyl transferase from Sulfolobus tokodaii

The structure of ST0929 provides insight into the structural basis of the increase in the hydrolase side reaction that is observed for mutants in which a phenylalanine residue is replaced by a tyrosine residue in the subsite +1 tyrosine cluster of Sulfolobus sp.

Crystallization and preliminary X-ray characterization of a catalytic and ATP-binding domain of a putative PhoR histidine kinase from the γ-radioresistant bacterium Deinococcus radiodurans

The expression, purification and crystallization of a catalytic and ATP-binding domain of PhoR histidine kinase from D. radiodurans is described.

Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli

E. coli D-alanyl-D-alanine ligase has been crystallized in complex with ADP and D-alanyl-D-alanine and in complex with ATP and D-alanyl-D-alanine.

Purification, crystallization and preliminary X-ray analysis of the aspartate aminotransferase of Plasmodium falciparum

The collection of X-ray diffraction data from the aspartate aminotransferase of P. falciparum to a resolution of 2.8 Å is reported.

Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a β-agarase from the flavobacterium Zobellia galactanivorans

A novel family GH16 β-agarase from the marine bacterium Zobellia galactanivorans was expressed, purified and crystallized. Hexagonal crystals belonging to space group P3₁21 diffracted to 2.2 Å resolution, whereas orthorhombic crystals belonging to space group P2₁2₁2₁ diffracted to 1.5–Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of a GroEL1 fragment from Mycobacterium tuberculosis H37Rv

The crystallization and preliminary X-ray crystallographic analysis of a 23 kDa GroEL1 fragment from M. tuberculosis H37Rv is reported.

The absence of inorganic salt is required for the crystallization of the complete oligomerization domain of Salmonella typhimurium histone-like nucleoid-structuring protein

Crystals of the oligomerization domain of the S. typhimurium histone-like nucleoid-structuring protein were obtained that diffracted X-rays to a resolution of at least 4.0 Å.

Purification, crystallization and preliminary crystallographic analysis of very-long-chain acyl-CoA de­hydrogenase from Caenorhabditis elegans

Very-long-chain acyl-CoA dehydrogenase from Caenorhabditis elegans (cVLCAD) has been crystallized in space group C2 and its X-ray diffraction data set has been collected to 1.6 Å resolution. Unlike other VLCADs that were reported to form dimers, the purified cVLCAD was found as a homotetrameric protein according to static light-scattering measurements.

Crystallization and initial X-ray diffraction studies of the flavoenzyme NAD(P)H:(acceptor) oxidoreductase (FerB) from the soil bacterium Paracoccus denitrificans

The flavin-dependent enzyme FerB from P. denitrificans has been purified and both native and SeMet-substituted FerB have been crystallized. The two variants crystallized in two different crystallographic forms belonging to the monoclinic space group P2₁ and the orthorhombic space group P2₁2₁2, respectively. X-ray diffraction data were collected to 1.75 Å resolution for both forms.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of rhesus macaque CD8αα homodimer

CD8α exodomain protein, a crucial immune-system factor in rhesus macaque (M. mulatta), one of the best animal models for vaccine design, was assembled and crystallized. The full structure data will contribute to future studies of immune responses in rhesus macaques.

Preliminary X-ray crystallographic analysis of the breakage–reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea strain 34H

The GyrA subunit of DNA gyrase from C. psychrerythraea strain 34H was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.60 Å.

Crystallization and X-ray diffraction studies of inverting trehalose phosphorylase from Thermoanaerobacter sp.

The inverting trehalose phosphorylase from Thermoanaerobacter sp. was crystallized and its structure was determined by molecular replacement. Its purification, crystallization and preliminary X-ray diffraction analysis are presented.

Crystallization of the pneumococcal autolysin LytC: in-house phasing using novel lanthanide complexes

The complete pneumococcal autolysin LytC has been crystallized by the hanging-drop vapor-diffusion method. A SAD data set has been collected in-house from a Gd derivative up to 2.6 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of the [NiFe]-hydrogenase maturation factor HypF1 from Ralstonia eutropha H16

The hydrogenase maturation factor HypF1 from R. eutropha H16 was successfully crystallized and data sets were collected to a maximum resolution of 1.65 Å.

Crystallization of the plant hormone receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid

Crystals of the Arabidopsis abscisic acid receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 have been obtained.

Crystallization and preliminary X-ray study of the D-altritol oligonucleotide GTGTACAC

The self-complementary octamer sequence GTGTACAC of the fully modified altritol nucleic acid has been crystallized. Diffraction data have been collected and processed to 3.0 Å resolution.

Purification, crystallization and crystallographic analysis of Dictyostelium discoideum phenylalanine hydroxylase in complex with di­hydrobiopterin and Fe^III

Phenylalanine hydroxylase from D. discoideum has been crystallized. Diffraction data were collected to 2.07 Å resolution.

Preliminary X-ray crystallographic analysis of 2-methylcitrate synthase from Salmonella typhimurium

2-Methylcitrate synthase from S. typhimurium was cloned, overexpressed, purified and crystallized. The crystals diffracted X-­rays to 2.4 Å resolution and belonged to the triclinic space group P1 (unit-cell parameters a = 92.068, b = 118.159, c = 120.659 Å, α = 60.84, β = 67.77, γ = 81.92°). Self-rotation function calculations suggested the presence of a putative decamer of protein subunits in the asymmetric unit.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-de­oxy-6-phosphogluconate aldolase from Zymomonas mobilis ZM4

The crystallization of 2-keto-3-deoxy-6-phosphogluconate aldolase from Z. mobilis ZM4, a key enzyme in ethanol production, and collection of diffraction data to 1.80 Å resolution are reported.

Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin

Equine cyanomethemoglobin has been crystallized and X-ray and neutron diffraction data have been measured. Joint X-ray–neutron refinement is under way; the structural results should help to elucidate the differences between the hemoglobin R and T states.

Efficient UV detection of protein crystals enabled by fluorescence excitation at wavelengths longer than 300 nm

Excitation of intrinsic fluorescence at wavelengths longer than 300 nm is effective in the detection of protein crystals in crystallization trials set up in the most commonly used hardware.