The structure of a family GH25 lysozyme from Aspergillus fumigatus

The X-ray structure of a fungal lysozyme shows a modified α/β-barrel-like fold and a likely active-site DXE motif.

Structure of a 14-3-3σ–YAP phosphopeptide complex at 1.15 Å resolution

The first structure of a 14-3-3 protein–phosphopeptide complex is reported at 1.15 Å resolution. The YAP 14-3-3-binding motif is revealed for the first time using crystallographic tools.

The structural plasticity of Tom71 for mitochondrial precursor translocations

Tom70 family members may exhibit structural plasticity when complexed with Hsp70/Hsp90. This structural plasticity enables Tom70 to accommodate various precursor substrates for mitochondrial translocation.

Near-atomic resolution analysis of BipD, a component of the type III secretion system of Burkholderia pseudomallei

The type III secretion system needle-tip protein BipD has been crystallized in a form that diffracts X-rays to 1.5 Å resolution and the structure has been refined to an R factor of 16.1% and an R_free of 19.8% at this resolution. The putative antiparallel dimer interface that was observed in earlier structures is conserved.

A new crystal form of Lys48-linked diubiquitin

A new crystal form of Lys48-linked diubiquitin was obtained and its structure was determined by X-ray crystallography to 1.6 Å resolution.

An inhibited conformation for the protein kinase domain of the Saccharomyces cerevisiae AMPK homolog Snf1

An inhibited conformation of the protein kinase domain (KD) of Snf1 is observed. The auto-inhibitory domain (AID) is disordered.

Structure of the endonuclease IV homologue from Thermotoga maritima in the presence of active-site divalent metal ions

When compared with their E. coli homologue, two X-ray crystal structures of T. maritima endonuclease IV differing in the composition of the trinuclear metal site point to the importance of the trinuclear site and its modulation among species to the function of this enzyme in the AP endonuclease IV family.

Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus

Structures of binary and ternary Dpo4 DNA polymerase complexes crystallized in the presence of Mg²⁺ have been determined. Comparisons with the corresponding Ca²⁺-form structures revealed subtle changes in the active-site geometry.

Structure of a stacked anthra­quinone–DNA complex

The molecular structure of a complex of an anthraquinone derivative and the oligonucleotide d(U^BrAGG) is presented. The anthraquinone molecules are stacked. Isolated base pairs are intercalated in the stack of drug molecules.

High-resolution structure of an α-spectrin SH3-domain mutant with a redesigned hydrophobic core

The structure of an α-spectrin SH3-domain mutant with a stabilized hydrophobic core and distal loop has been solved at 1 Å resolution.

Comparison between the orthorhombic and tetragonal forms of the heptamer sequence d[GCG(xT)GCG]/d(CGCACGC)

The crystal structure of the tetragonal form of the non-self-complementary DNA sequence d[GCG(xT)GCG]/d(CGCACGC) with one incorporated CeNA (xT) moiety has been solved and is compared with the high-resolution orthorhombic form.

Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2

The structure of Ca²⁺-bound EF-hand protein S100A2 was determined by calcium and sulfur SAD at a wavelength of 0.90 Å.

Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of bitter gourd (Momordica charantia)

A galactose-specific lectin purified from the seeds of bitter gourd (M. charantia) has been crystallized and preliminary X-ray study of the crystals has been carried out.

Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum

The catalytic domain of CHBI was purified from a cellular extract of T. harzianum. Diffraction-quality crystals were obtained and a native X-ray data set was collected using a synchrotron source.

Crystallization and preliminary X-ray analysis of PaaAC, the main component of the hydroxylase of the Escherichia coli phenylacetyl-coenzyme A oxygenase complex

The expression, purification, crystallization and preliminary crystallographic analysis of the PaaAC complex is reported. This is the main component of the E. coliphenylacetyl-coenzyme A oxygenase complex.

Crystallization and preliminary X-ray crystallographic analysis of the ArsM arsenic(III) S-adenosylme­thionine methyltransferase

A common biotransformation of arsenic is methylation to monomethylated, dimethylated and trimethylated species, which is catalyzed by the ArsM (or AS3MT) arsenic(III) S-adenosylmethionine methyltransferase. ArsM from the acidothermophilic alga Cyanidioschyzon sp. 5508 was expressed, purified and crystallized by the hanging-drop vapor-diffusion method and diffraction data were collected to 1.76 Å resolution.

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of 5-aminolaevulinic acid dehydratase from Bacillus subtilis

Crystals of 5-aminolaevulinic acid dehydratase from B. subtilis are tetragonal, belonging to space group P4₂2₁2, and diffract to 2.7 Å resolution.

Crystallization and preliminary crystallographic characterization of the PAS domains of EAG and ELK potassium channels

The N-terminal PAS domains from the eukaryotic EAG potassium channels are thought to have a regulatory function. Here the expression, purification, crystallization and preliminary crystallographic characterization of two of these domains are described.

Preliminary crystallographic analysis of the Escherichia coli antitoxin MqsA (YgiT/b3021) in complex with mqsRA promoter DNA

The E. coli antitoxin MqsA (YgiT/b3021) has been cocrystallized with mqsRA promoter DNA. A native data set has been collected to a resolution of 2.1 Å.

Crystallization and preliminary X-ray analysis of formate oxidase, an enzyme of the glucose–methanol–choline oxidoreductase family

Formate oxidase from A. oryzae RIB40 was crystallized and diffraction data were collected to a resolution of 2.4 Å.

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of a human condensin SMC2 hinge domain with short coiled coils

In this study, the human condensin SMC2 hinge domain with short coiled coils was cloned, expressed, purified and crystallized in the orthorhombic space group C222 in native and SeMet-derivatized forms.

Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region

In this study, the core region of Ara h 1, one of the major peanut allergens, has been overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.25 Å resolution.

The Corynebacterium glutamicum aconitase repressor: scratching around for crystals

Crystallization of AcnR, a repressor of the aconitase gene in Corynebacterium glutamicum, is reported. Intentional manual scratching of the crystallization plates was applied to induce heterogeneous nucleation.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of the catalytic domain of a hyperthermostable endo-1,4-β-D-mannanase from Thermotoga petrophila RKU-1

The catalytic domain of a hyperthermostable endo-1,4-β-D-mannanase from T. petrophila RKU-1 has been cloned, overexpressed in E. coli cells, purified and crystallized in two distinct crystalline forms by the sitting-drop vapour-diffusion method.

Crystallographic study of wild-type carbonic anhydrase αCA1 from Chlamydomonas reinhardtii

The carbonic anhydrase αCA1 from C. reinhardtii is a dimeric class αCA enzyme with post-translational glycosylation at three asparagine residues and proteolytic removal of a short peptide. The mature enzyme has been crystallized, MAD data have been collected to 1.88 Å resolution and a preliminary solution of the crystal structure has been obtained.

Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans

In this study, the glucansucrase from the dental caries pathogen S. mutans was purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant.

Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase

Cu,Zn superoxide dismutase from the thermophilic fungus C. thermophilum was expressed in P. pastoris, purified and crystallized. A complete data set was collected to 1.9 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray studies of the C-terminal domain of Mycobacterium tuberculosis LexA

The C-terminal domain of M. tuberculosis LexA has been crystallized and its structure has been solved.

Preliminary crystallographic study of the Streptococcus agalactiae sortases, sortase A and sortase C1

The expression, purification, crystallization and preliminary X-ray diffraction analysis of pili-specific and housekeeping sortases from S. agalactiae are reported.

Crystallization and preliminary X-ray crystallographic analysis of a bacterial tyrosinase from Bacillus megaterium

The crystallization and primary crystallographic analysis of B. megaterium tyrosinase is reported. The purified protein was crystallized in the absence or presence of zinc ions to a resolution of 2.0 Å.

Crystallization and preliminary X-ray analysis of mycophenolic acid-resistant and mycophenolic acid-sensitive forms of IMP de­hydrogenase from the human fungal pathogen Cryptococcus

IMP dehydrogenase from C. neoformans and C. gattii has been crystallized in complex with the substrate IMP and the cofactor NAD⁺.

Cloning, purification, crystallization and preliminary X-ray diffraction of the OleC protein from Stenotrophomonas maltophilia involved in head-to-head hydrocarbon biosynthesis

OleC crystals belonged to space group P3₁21 or P3₂21 and diffracted to 3.4 Å resolution at a third-generation synchrotron X-ray source.

Preliminary crystallographic analysis of the N-terminal domain of FILIA, a protein essential for embryogenesis

The preliminary crystallographic analysis of the N-terminal domain of FILIA is described in this paper. FILIA is a component of subcortical maternal complex, which plays critical roles in embryogenesis.

Crystallization and preliminary X-ray crystallographic study of GenX, a lysyl-tRNA synthetase paralogue from Escherichia coli, in complex with translation elongation factor P

GenX, a lysyl-tRNA synthetase paralogue from Escherichia coli, has been overexpressed in E. coli, purified by three chromatographic steps and cocrystallized with a lysyl adenylate analogue (LysAMS) by the hanging-drop vapour-diffusion method using PEG 4000 as a precipitant.

Crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein FrpD from Neisseria meningitidis

Native FrpD_43–271 and selenomethionine-derivative FrpD_43–271 proteins were purified, crystallized and diffracted to resolutions of 2.25 and 2.00 Å, respectively.

Expression, purification and crystallization of Swi5 and the Swi5–Sfr1 complex from fission yeast

The crystallization of the homologous recombination mediators Swi5 and Sfr1 from fission yeast is reported.

Crystallization and preliminary X-ray diffraction analysis of rat autotaxin

Autotaxin (ATX), a pyrophosphatase/phosphodiesterase enzyme, is a promising drug target for many indications and is only distantly related to enzymes of previously determined structure. Here, the cloning, expression, purification, crystallization and preliminary diffraction analysis of ATX are reported.

Mammalian cell expression, purification, crystallization and microcrystal data collection of autotaxin/ENPP2, a secreted mammalian glycoprotein

Autotaxin, a four-domain ∼100 kDa mammalian glycoprotein, was expressed in stably transfected mammalian cells, purified from the medium and crystallized. Diffraction data from micrometre-thick crystal plates were collected on various European synchrotron beamlines and are presented and analysed.