Structure of GlnK1, a signalling protein from Archaeoglobus fulgidus

The structure of GlnK1, one of three GlnK proteins encoded by the hyperthermophilic archaeon A. fulgidus, was solved to 2.28 Å resolution.

Structure of the putative di­hydroorotate de­hydrogenase from Streptococcus mutans

The crystal structure of SMU.595, a putative dihydroorotate dehydrogenase (DHOD) from S. mutans, is reported at 2.4 Å resolution.

Structure of the catalytic domain of glucoamylase from Aspergillus niger

A crystal structure of the catalytic domain of glucoamylase from A. niger with Tris and glycerol bound in the active site has been solved by molecular replacement and refined to 1.9 Å resolution.

The structure of a haemopexin-fold protein from cow pea (Vigna unguiculata) suggests functional diversity of haemopexins in plants

The structure of CP4, a haemopexin-fold protein from cow pea (Vigna unguiculata), has been determined at 2.1 Å resolution.

Structure of the dimeric form of CTP synthase from Sulfolobus solfataricus

The crystal structure of an unliganded form of CTP synthase from S. solfataricus unambiguously establishes that while the active form of the enzyme is tetrameric, a dimeric form has been crystallized. A comparison with the tetrameric form of the E. coli enzyme suggests that rather large intramolecular movements accompany tetramerization.

Structure of flap endonuclease 1 from the hyperthermophilic archaeon Desulfuro­coccus amylolyticus

The crystal structure of flap endonuclease 1 from the hyperthermophilic archaeon D. amylolyticus was determined at 2.00 Å resolution.

Crystallization and preliminary X-ray crystallographic studies of enoyl-acyl carrier protein reductase (FabI) from Psuedomonas aeruginosa

Enoyl-acyl carrier protein reductase (FabI) from P. aeruginosa was purified and crystallized. FabI was also cocrystallized with the inhibitor triclosan and the cofactor NADH. Crystals of native and complexed FabI diffracted to resolutions of 2.6 and 1.8 Å, respectively.

Crystallization and preliminary crystallographic analysis of β-mannanase from Bacillus licheniformis

The expression, purification, crystallization and preliminary crystallographic analysis of recombinant β-mannanase from B. licheniformis strain DSM13 are described.

Crystallization and preliminary X-ray analysis of the human long myosin light-chain kinase 1-specific domain IgCAM3

To gain insight into the structure of the IgCAM3 domain, the IgCAM3 domain of MLCK1 has been expressed, purified and crystallized.

Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae

The thermostable direct haemolysin from G. hollisae has been purified and crystallized in two crystal forms using the vapour-diffusion method.

Crystallization and preliminary crystallographic studies of a cysteine protease inhibitor from the human nematode parasite Ascaris lumbricoides

A recombinant cysteine protease inhibitor from the human nematode parasite A. lumbricoides has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.1 Å resolution.

Crystallization and preliminary X-ray crystallographic studies of β-transaminase from Mesorhizobium sp. strain LUK

β-Transaminase from Mesorhizobium sp. strain LUK was crystallized. The crystals were found to belong to the orthorhombic space group C222₁, with unit-cell parameters a = 90.91, b = 192.17, c = 52.75 Å. The crystals were obtained at 293 K and diffracted to a resolution of 2.5 Å.

Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

Wild-type and H12A-mutant class II phospholipase D from L. intermedia venom were crystallized; the crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively.

Crystallization, X-ray diffraction analysis and preliminary structure determination of the TIR domain from the flax resistance protein L6

This report describes the crystallization and preliminary structure determination of the TIR domain from a plant disease-resistance protein.

Purification, crystallization and preliminary X-ray diffraction of a di­sulfide cross-linked complex between bovine poly(A) polymerase and a chemically modified 15-mer oligo(A) RNA

A disulfide cross-linked complex between bovine poly(A) polymerase and a chemically modified RNA was crystallized. X-ray diffraction data were collected to 2.25 Å resolution from crystals that belonged to space group P2.

Crystallization and preliminary crystallographic analysis of Arabidopsis thaliana EDS1, a key component of plant immunity, in complex with its signalling partner SAG101

The central plant immune regulator EDS1 (enhanced disease susceptibility1) from A. thaliana has been crystallized for the first time. Crystals of EDS1 in complex with its signalling partner SAG101 (senescence-associated gene101) diffracted to 3.5 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of a Lys49-phospholipase A₂ complexed with caffeic acid, a molecule with inhibitory properties against snake venoms

Piratoxin I, a noncatalytic and myotoxic Lys49-phospholipase A₂ from B. pirajai venom, was cocrystallized with the inhibitor caffeic acid and a data set was collected to a resolution of 1.65 Å. The electron-density map unambiguously indicated that three inhibitor molecules interact with the C-­terminus of the protein.

Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands

Crystals of the wild-type haloalkane dehalogenase DhaA derived from R. rhodochrous NCIMB 13064 and of its catalytically inactive variant DhaA13 were grown in the presence of various ligands and diffraction data were collected to high and atomic resolution.

Preliminary joint neutron time-of-flight and X-ray crystallographic study of human ABO(H) blood group A glycosyltransferase

Collection to 2.5 Å resolution of neutron diffraction data from crystals of the human ABO(H) blood group glycosyltransferase GTA, is presented with the preliminary joint refinement with the corresponding X-ray diffraction data.

Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori

Octaprenyl pyrophosphate synthase from H. pylori has been expressed, purified and crystallized, and a diffraction data set was collected to 2.00 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of Lmo0540 from Listeria monocytogenes

The crystallization of Lmo0540 from L. monocytogenes is reported.

Purification, crystallization and preliminary crystallographic analysis of the SH2 domain of IL-2-inducible T-cell kinase

Crystallization conditions are described for the cis- and trans-imide bond-containing SH2 domain of IL-2-inducible T-cell kinase.

Crystallization and initial crystallographic analysis of the Streptococcus parasanguinis FW213 Fap1-NR_α adhesive domain at pH 5.0

The adhesin fimbriae-associated protein 1 (Fap1) is a surface protein of Streptococcus parasanguinis FW213 and plays a major role in the formation of dental plaque in humans. Here, the adhesive domain Fap1-NR_α, which is activated by acidic pH, has been crystallized at pH 5.0 and diffraction data have been collected to 3.0 Å resolution.

Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the C-terminal domain of the GyrA subunit of DNA gyrase from Staphylococcus aureus strain Mu50

The C-terminal domain of the DNA gyrase GyrA from S. aureus strain Mu50 was cloned, overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.80 Å.

Purification, crystallization and preliminary X-ray crystallographic analysis of the C-terminal cytoplasmic domain of FlhB from Aquifex aeolicus

The cytoplasmic domain of FlhB from A. aeolicus has been purified and crystallized.

Preliminary joint X-ray and neutron protein crystallographic studies of endoxylanase II from the fungus Trichoderma longibrachiatum

A fungal family 11 endoxylanase has been crystallized at pH 8.5 and room-temperature X-ray and neutron diffraction data have been collected. Joint X-ray/neutron refinement is under way; the structural results will aid in rational engineering of the enzyme.

Expression, crystallization and preliminary X-ray analysis of the phosphoribosylglycinamide formyltransferase from Streptococcus mutans

Phosphoribosylglycinamide formyltransferase (PurN) from Streptococcus mutans was expressed in E. coli, purified and studied crystallographically.

Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid

Intestinal fatty-acid binding proteins from human and rat have been crystallized in complex with the fluorescent probe 11-(dansylamino)undecanoic acid. Diffraction data for the crystals were collected to 1.8 Å resolution (human) and 1.6 Å resolution (rat).