Structure of CBM4 from Clostridium thermocellum cellulase K

The crystal structure of C. thermocellum CelK CMB4 confirms unique extended binding pocket features of clostridial CBM4s.

Structure of the uncomplexed Neisseria meningitidis factor H-binding protein fHbp (rLP2086)

Here, the crystal structure of mature fHbp determined at 2 Å resolution is presented and is compared with the structure of the same protein in complex with factor H domains 6 and 7 recently solved using X-ray techniques.

Structural analysis of full-length Hfq from Escherichia coli

The full-length RNA-chaperone Hfq from Escherichia coli (102 amino acids) has been crystallized. The crystal belongs to the P1 space group. The structure was solved to 2.85 Å resolution, revealing that the disordered C-terminal resides on the proximal face of the hexamer.

Structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase in complex with the feedback inhibitor CoA reveals only one active-site conformation

The X-ray structure of phosphopantetheine adenylyltransferase (PPAT) from M. tuberculosis in complex with its feedback regulator coenzyme A (CoA) was determined to 2.11 Å resolution. Unlike previous X-ray structures of PPAT–CoA complexes from other bacteria, which showed two distinct conformations of bound CoA, only one conformation of bound CoA is observed in the M. tuberculosis PPAT–CoA complex.

Preliminary crystallographic analysis of the RNA-binding domain of HuR and its poly(U)-binding properties

Here, the recombinant ARE-binding region of HuR (residues 18–186) was crystallized in space group P2₁2₁2, with unit-cell parameters a = 41.2, b = 133.1, c = 31.4 Å.

Purification, crystallization and preliminary data analysis of the ligand–receptor complex of the growth and differentiation factor 5 variant R57A (GDF5R57A) and BMP receptor IA (BRIA)

The ligand–receptor complex of human growth and differentiation factor 5 (GDF5) bound to the extracellular domain of human BMP type IA receptor (BRIA_EC) can be crystallized as long as the GDF5 variant R57A, which exhibits an enhanced affinity for BRIA, is employed.

Crystallization of an engineered RUN domain of Rab6-interacting protein 1/DENND5

An engineered deletion of a nonconserved loop in the RUN domain of Rab6-interacting protein 1 enhances the solubility of the recombinant protein when expressed in E. coli. Crystals of the engineered effector in complex with Rab6a diffracted X-rays to 3.25 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of the barley yellow dwarf virus cap-independent translation element

The sequence design, purification, crystallization and preliminary X-ray diffraction analysis of the novel cap-independent translation RNA element from the 3′ untranslated region of the barley yellow dwarf virus genome is reported.

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of NifH1 from Methanocaldococcus jannaschii

Solving the structure of MjNifH1 may help in better understanding its function and may supply some clues to understanding the evolution of nitrogenase. The full-length protein with an additional His₆ tag at the C-terminus was expressed, purified and crystallized by the hanging-drop vapour-diffusion method at 287 K.

Complex assembly, crystallization and preliminary X-ray crystallographic studies of the swine major histocompatibility complex molecule SLA-1*1502

A crystal of a swine MHC class I molecule diffracted to 2.2 Å resolution and belonged to space group P2₁2₁2₁, with unit-cell parameters a = 66.1, b = 74.1, c = 98.6 Å; it contained one molecule in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.74 ų Da⁻¹ and 55.17%, respectively.

Crystallization and diffraction analysis of Sm23: an SGNH-family arylesterase from Sinorhizobium meliloti 1021

Sm23, a novel SGNH arylesterase from S. meliloti 1021, was crystallized in space group I4₁22 and diffraction data were collected to a resolution of 2.2 Å.

Crystallization and preliminary X-ray crystallographic analysis of tyrosinase from the mushroom Agaricus bis­porus

Mushroom tyrosinase was crystallized in two different space groups. The crystals diffracted to 3.0 Å resolution (P2₁) or 2.6 Å resolution (P2₁2₁2).

Purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda

Phycocyanin and phycoerythrin from P. yezoensis Ueda were prepared using co-precipitation with ammonium sulfate and hydroxyapatite column chromatography with improved purity and yield. The preliminary X-ray analysis clarified the ambiguity in the unit-cell contents resulting from the Matthews coefficient calculation and the final model showed that phycocyanin is present as two α₆β₆ heterododecamers in the asymmetric unit associated by an NCS twofold axis.

Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum

The transaldolase enzyme from T. acidophilum has been crystallized in two different space groups.

Time-of-flight neutron diffraction study of bovine γ-chymotrypsin at the Protein Crystallography Station

In order to begin an exact determination of hydrogen positions in proteins, a neutron diffraction study of bovine gamma-chymotrypsin has been conducted. This paper details the data collection of the protein at pD (pH*) 7.1.

Superoxide reductase from Nanoarchaeum equitans: expression, purification, crystallization and preliminary X-ray crystallographic analysis

Diffraction-quality crystals of N. equitans neelaredoxin have been produced. The expression, purification and crystallization of the protein and preliminary X-ray crystallographic analysis of the crystals are reported.

Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis

The mannose-binding lectin domain of MSMEG_3662 from M. smegmatis has been crystallized and preliminary X-ray studies have been carried out on the crystals.

Crystallization and preliminary diffraction analysis of the CAL PDZ domain in complex with a selective peptide inhibitor

A CAL PDZ-domain construct was crystallized in complex with the selective peptide inhibitor iCAL36, which has been shown to enhance ΔF508-CFTR chloride-channel activity in human airway epithelial cells.

Purification, crystallization and preliminary X-ray crystallographic analysis of the putative Vibrio parahaemolyticus resuscitation-promoting factor YeaZ

The V. parahaemolyticus resuscitation-promoting factor YeaZ has been purified and crystallized by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 3.1 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of MIL, a glycosylated jacalin-related lectin from mulberry (Morus indica) latex

The purification, preliminary characterization and crystallization of a lectin purified from M. indica latex are presented. Using synchrotron radiation, a diffraction data set to 2.8 Å resolution was collected.

Crystallization and preliminary X-ray analysis of the N-terminal domain of human thioredoxin-interacting protein

The N-terminal domain of thioredoxin-interacting protein has been expressed, purified and crystallized. The crystals belonged to a monoclinic space group and diffracted to 3 Å resolution using synchrotron radiation.

Molecular cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a purine nucleoside phosphorylase from Bacillus subtilis strain 168

Recombinant purine nucleoside phosphorylase from B. subtilis strain 168 has been crystallized at different pH values using the sitting-drop vapour-diffusion method.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

Cloning, expression, purification and X-ray data collection of the RadA intein from the hyperthermophilic archaebacterium P. horikoshii are reported.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of Rv3168 from Mycobacterium tuberculosis H37Rv

The gene product Rv3168, a putative aminoglycoside phosphotransferase (APH), from Mycobacterium tuberculosis has been crystallized using the sitting-drop vapour-diffusion method and X-ray diffraction data were collected to a maximum resolution of 1.67 Å on a synchrotron beamline.

Crystallization and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter haemolysin B

Here, the overexpression, purification and crystallization of the isolated C39 peptidase-like domain of the Escherichia coli ABC transporter haemolysin B are described.

Crystallization and preliminary X-ray diffraction studies of the precursor protein of a thermostable variant of papain

The crystallization of the precursor of a thermostable variant of papain and the collection of diffraction data to 2.6 Å resolution are reported.

Protein expression, crystallization and preliminary X-ray crystallographic studies of LidA from Legionella pneumophila

The crystallization of recombinant lidA, a translocated substrate of the Legionella pneumophila Dot/Icm type IV secretion system, is reported. Crystals were obtained and diffracted to 2.75 Å in space group P2₁2₁2₁.