The host-binding domain of the P2 phage tail spike reveals a trimeric iron-binding structure

The C-terminal domain of a bacteriophage P2 tail-spike protein, gpV, was crystallized and its structure was solved at 1.27 Å resolution. The refined model showed a triple β-helix structure and the presence of iron, calcium and chloride ions.

In silico identification and crystal structure validation of caspase-3 inhibitors without a P1 aspartic acid moiety

Structure-based identification and validation by protein X-ray crystallography of halomethyl ketone derivatives that inhibit caspase-3 without an aspartic acid in the P1 position is reported.

Structure of the RuBisCO chaperone RbcX from the thermophilic cyanobacterium Thermosynechococcus elongatus

The crystal structure of the RuBisCO assembly chaperone RbcX from a thermophilic cyanobacterium has been determined at 1.7 Å resolution. The dimeric structure is capable of a hinge movement (probably connected with binding of the RuBisCO large subunit) pivoted on a kink in two long antiparallel α-helices.

Conservation of a crystallographic interface suggests a role for β-sheet augmentation in influenza virus NS1 multifunctionality

The structure of a monomeric effector domain from influenza A virus NS1 is presented from diffraction data extending to 1.8 Å resolution. Comparison of this and other NS1 effector-domain structures shows conformational changes at a strand–strand packing interface, hinting at a role for β-strand augmentation in NS1 function.

Structure of saxthrombin, a thrombin-like enzyme from Gloydius saxatilis

Saxthrombin, a snake-venom thrombin-like enzyme from Gloydius saxatilis, was purified and crystallized to obtain a high-quality crystal, from which data were acquired to 1.43 Å resolution.

Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0

The crystal structure of the M. tuberculosis soluble inorganic pyrophosphatase Rv3628 determined at pH 7.0 reveals conformational changes compared with the structure at pH 5.0. The conformational changes arise not only as a consequence of the different pH but also of the presence of two potassium ions bound to the protein.

Structure of filamin A immunoglobulin-like repeat 10 from Homo sapiens

The structure of immunoglobulin-like repeat 10 from human filamin A solved at 2.44 Å resolution suggests the potential effects of mutations correlated with otopalatodigital syndrome spectrum disorders.

Conformational bias imposed by source microseeds results in structural ambiguity

The p38αY323T active mutant crystallized in two different forms representing different structural features. It has been shown that the crystal profile and the resultant structures are biased by the source microseeds.

Preliminary X-ray crystallographic studies of UDP-glucose-4-epimerase from Aspergillus nidulans

UDP-glucose-4-epimerase from A. nidulans has been crystallized as a complex with the substrate UDP-galactose using the microbatch method under paraffin oil.

Crystallization and preliminary X-ray crystallographic studies of swine CD8α

Crystals of swine CD8α diffracted to 1.8 Å resolution and belonged to space group P3₂21, with unit-cell parameters a = 80.97, b = 80.97, c = 95.19 Å; they contained two molecules in the asymmetric unit. The Matthews coefficient and solvent content were calculated to be 3.23 ų Da⁻¹ and 61.89%, respectively.

Crystallization and X-ray diffraction analysis of nylon-oligomer hydrolase (NylC) from Agromyces sp. KY5R

Crystals of 6-aminohexanoate-oligomer hydrolase have been obtained by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. Diffraction data for native and K₂PtCl₄-derivative crystals were collected to resolutions of 2.00 and 2.20 Å, respectively.

Purification and crystallization of yeast glycosylphosphatidylinositol transamidase subunit PIG-S (PIG-S_71–467)

The PIG-S_71–467 subunit of the yeast GPI transamidase complex was cloned, overexpressed, purified and crystallized. The crystals belonged to space group C2 and diffracted to 3.2 Å resolution.

Crystallization and preliminary X-ray diffraction studies of BmooPLA₂-I, a platelet-aggregation inhibitor and hypotensive phospholipase A₂ from Bothrops moojeni venom

BmooPLA₂-I, an acidic, catalytic and nontoxic phospholipase A₂ from B. moojeni venom that is able to inhibit platelet aggregation and induce a hypotensive effect, has been crystallized. An X-ray diffraction data set was collected to 1.6 Å resolution and a molecular-replacement solution was obtained.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a 12R-LOX–chaperone complex

Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a 12R-LOX–chaperone complex are reported. The crystals belonged to the space group P2₁ and diffracted to 4 Å resolution.

Crystallization and preliminary crystallographic analysis of a PHD domain of human JARID1B

The expression, purification and crystallization of a PHD domain of human JARID1B are described.

Crystallization and preliminary X-ray analysis of a cold-active β-galactosidase from the psychrotrophic and halotolerant Planococcus sp. L4

β-Galactosidase from the psychrotrophic and halotolerant Planococcus sp. L4 (BgaP) was crystallized and a complete data set was collected. There are six protein subunits in the asymmetric unit.

Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose

Glucokinase from S. griseus (SgGlkA) was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. A crystal of SgGlkA in complex with glucose diffracted X-rays to 1.84 Å resolution.

Crystallization and diffraction analysis of thioredoxin reductase from Streptomyces coelicolor

Thioredoxin reductase from S. coelicolor was crystallized and diffraction data were collected to 2.4 Å resolution.

Preliminary crystallographic analysis of a possible transcription factor encoded by the mimivirus L544 gene

The mimivirus L544 gene product was expressed in E. coli and crystallized; preliminary phasing of a MAD data set was performed using the selenium signal present in a crystal of recombinant selenomethionine-substituted protein.

Purification, crystallization and preliminary X-ray structure analysis of the laccase from Ganoderma lucidum

The purification, crystallization and preliminary X-ray structure analysis of the laccase from G. lucidum are reported.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

P. furiosus PF2050 has been crystallized. Diffraction data were collected to 1.56 Å resolution using synchrotron X-rays.

Expression, purification and preliminary crystallographic analysis of Rv3002c, the regulatory subunit of acetolactate synthase (IlvH) from Mycobacterium tuberculosis

The small regulatory subunit of acetohydroxylate synthase (IlvH) from M. tuberculosis has been crystallized and preliminary crystallographic analysis has been performed.

Purification, crystallization and preliminary X-ray crystallographic analysis of the receiver and stalk domains (PA3346RS) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1

The crystallization of PA3346RS from P. aeruginosa is reported.

Expression, purification and preliminary crystallographic analysis of human thyroid hormone responsive protein

Human Thrsp has been crystallized as a prelude to the determination of its three-dimensional structure by X-ray crystallography.

Purification, crystallization and preliminary X-ray diffraction analysis of the human mismatch repair protein MutSβ

Human MutSβ is a 232 kDa heterodimer (MSH2–MSH3) involved in the lesion-recognition step of mismatch repair. Here, the overexpression, purification, biochemical characterization and cocrystallization of MutSβ with a duplex DNA substrate are reported.

Crystallization and preliminary X-ray analysis of the yeast tRNA-thiouridine modification protein 1 (Tum1p)

Yeast tRNA-thiouridine modification protein 1 was overpressed in E. coli, purified and crystallized. The crystals belonged to space group I4₁ and diffracted to a resolution of 1.9 Å.

Purification, crystallization and preliminary crystallographic analysis of recombinant Lac15 from a marine microbial metagenome

Lac15 from a marine microbial metagenome has been crystallized and preliminary crystallographic analysis has been performed.

Expression, purification and preliminary crystallographic analysis of O-acetylhomoserine sulfhydrylase from Mycobacterium tuberculosis

O-Acetylhomoserine sulfhydrylase from M. tuberculosis H37Rv has been crystallized and preliminary X-ray crystallographic analysis has been performed.

Crystallization and preliminary X-ray analysis of CYP153C1 from Novosphingobium aromaticivorans DSM12444

The expression, purification and preliminary crystallographic analysis of CYP153C1, an alkane-binding cytochrome P450 enzyme, are reported.

Expression, purification, crystallization and preliminary crystallographic analysis of Cg1458: a novel oxalo­acetate de­carboxylase from Corynebacterium glutamicum

To elucidate the mechanism of oxaloacetate decarboxylation by Cg1458, recombinant Cg1458 has been purified and crystallized.

From screen to structure with a harvestable microfluidic device

Microfluidic crystallization using the Crystal Former improves the identification of initial crystallization conditions relative to screening via vapour diffusion.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of glucuronic acid dehy­drogenase from Chromohalobacter salexigens. Addendum

An addendum to the article by Ahn et al. [Acta Cryst. (2011) F67, 689–691].

Crystallization and preliminary X-ray crystallographic studies of the ice-binding protein from the Antarctic yeast Leucosporidium sp. AY30. Corrigendum

A correction to the article by Park et al. [(2011). Acta Cryst. F67, 800–802].