The precursor form of Hansenula polymorpha copper amine oxidase 1 in complex with Cu^I and Co^II

The structure of copper amine oxidase 1 from H. polymorpha in its metal-free precursor (apo) form is reported along with structures of the apo protein in complex with Cu^I and Co^II.

Structure of a hexameric form of RadA recombinase from Methanococcus voltae

Hexameric rings of RadA recombinase from M. voltae have been crystallized. Structural comparisons suggest that homologues of RadA tend to form double-ringed assemblies.

New crystal structure of the proteasome-dedicated chaperone Rpn14 at 1.6 Å resolution

A new crystal structure of yeast Rpn14 with an E384A mutation was determined at 1.6 Å resolution. The improved high-resolution structure provides a framework for understanding proteasome assembly.

The structure of an orthorhombic crystal form of a `forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag

The crystal structure of the C61S mutant of Tpx from E. coli is reported at 1.97 Å resolution. A brief structural comparison with homologues is presented.

Structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form: insights into the path of carbamoyl phosphate to the active site of the enzyme

The structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase has been determined in a hexagonal crystal form and gives insight into the possible paths that the substrate carbamoyl phosphate may follow to reach the active site during catalysis.

Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa

Initial crystallographic studies of the X. fastidiosa small heat-shock protein HSP17.9 are reported.

Expression, purification, crystallization and preliminary X-ray analysis of carbonyl reductase S1 from Candida magnoliae

Carbonyl reductase S1 from C. magnoliae was expressed, purified and crystallized. The crystals obtained diffracted X-rays to 1.90 Å resolution and belonged to space group P6₁22 or P6₅22.

Crystallization and preliminary X-ray diffraction analysis of the SOD-TAT fusion protein

A cell membrane permeable SOD, SOD-TAT fusion protein, was expressed, purified and crystallized. X-ray diffraction data have been collected to 3.2 Å resolution.

Expression, purification, crystallization and preliminary crystallographic studies of Rhagium inquisitor antifreeze protein

A novel hyperactive antifreeze protein from R. inquisitor (RiAFP) has been overexpressed, purified and crystallized. A complete native X-ray diffraction data set was recorded to 1.3 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid Δ¹-de­hydrogenase from Rhodococcus erythropolis SQ1

3-Ketosteroid Δ¹-dehydrogenase from Rhodococcus erythropolis SQ1 was successfully crystallized and its initial structure was solved.

Crystallization and preliminary X-ray crystallographic analysis of the me­thionine sulfoxide reductase A domain of MsrAB from Haemophilus influenzae

The MsrA domain of MsrAB from H. influenzae was crystallized. X-ray diffraction data were collected to 1.6 Å resolution.

Identification, crystallization and preliminary X-ray diffraction analysis of esterase A from Caulobacter crescentus CB15, a family VIII lipolytic enzyme

Esterase A from C. crescentus CB15 was crystallized in space group C222₁ and diffraction data were collected to a resolution of 1.62 Å.

Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans

Two hypothetical ribose-5-phosphate isomerases from S. mutans have been produced in E. coli and crystallized. The crystals diffracted to high resolutions suitable for crystallographic analyses.

Expression, purification and crystallization of the Crimean–Congo haemorrhagic fever virus nucleocapsid protein

The Crimean–Congo haemorrhagic fever virus nucleocapsid protein was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to 2.1 Å resolution.

Crystallization and preliminary crystallographic study of a trypsin-resistant catalytic domain of human calcineurin

A trypsin-resistant catalytic domain of human calcineurin α (A subunit, residues 20–347) was crystallized in space group P2₁2₁2. An X-ray diffraction data set was collected to 2.87 Å resolution and the structure was solved by molecular replacement.

Crystallization and preliminary crystallographic analysis of the major capsid proteins VP16 and VP17 of bacteriophage P23-77

The major capsid proteins VP16 and VP17 of bacteriophage P23-77 have been crystallized using both recombinant and purified virus and preliminary diffraction analyses have been performed.

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgL

The expression, purification and crystallization of the alginate lyase AlgL from P. aeruginosa is described. The crystals diffracted to a resolution of 1.64 Å.

Crystallization and preliminary X-ray crystallographic analysis of hydro­quinone dioxygenase from Sphingomonas sp. TTNP3

Crystals of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3 belonged to space group P2₁ and contained two heterotetramers in the asymmetric unit related by 222 noncrystallographic symmetry. X-ray data were collected to a maximum resolution of 2.5 Å using a synchrotron source.

Crystallization and preliminary characterization of chloromuconolactone dehalogenase from Rhodococcus opacus 1CP

The crystallization and preliminary X-ray diffraction analysis of a novel chloromuconolactone dehalogenase from R. opacus 1CP are described. The oligomeric state was determined based on the self-rotation function.

Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

The utility of differential scanning fluorimetry for homogeneity assessment and crystallization improvement of PLP-dependent enzymes is demonstrated using the potential drug target BioA from M. tuberculosis.

On-column ligand exchange for structure-based drug design: a case study with human 11β-hydroxysteroid de­hydrogenase type 1

An on-column ligand- and detergent-exchange method was developed to obtain ligand–protein complexes for an adamantane series of compounds with 11β-HSD1 after a variety of other complexation methods had failed. An interesting byproduct of the method was the observation of artificial trimers in the crystal structures.

`Seeding' with protease to optimize protein crystallization conditions in in situ proteolysis

Addition of protease instead of seeds using a robot can be used to optimize the concentration of protease in in situ proteolysis experiments and has been successfully tested using two proteins.