A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus

The structure of 3-methyladenine DNA glycosylase I in complex with 3-methyladenine is reported.

Structure of human ADAM-8 catalytic domain complexed with batimastat

The catalytic domain of human ADAM-8 was expressed, purified and crystallized in complex with a hydroxamic acid inhibitor, batimastat. The crystal structure of the enzyme–inhibitor complex was refined to 2.1 Å resolution.

Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution

The structure of the L166K variant of G protein-coupled receptor kinase 1 has been determined at 2.5 Å resolution in order to determine how a dimer interface observed in prior crystal structures influences the conformation of the enzyme and how the C-terminal amino acids are configured while in a monomeric state.

Structure of the GDP-bound G domain of the RGK protein Rem2

The structure of the Rem2 G domain bound to GDP is reported in a monoclinic crystal form at 2.66 Å resolution.

Structure of a short-chain de­hydrogenase/reductase from Bacillus anthracis

The crystal structure of a short-chain dehydrogenase/reductase from B. anthracis strain `Ames Ancestor' is reported.

Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site

MatP is a small DNA-binding protein of about 18 kDa. In order to understand the DNA-compaction mechanism of MatP at an atomic level, the structures of apo MatP and of the nucleoprotein complex MatP–matS have been studied.

Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney

D-Aspartate oxidase from porcine kidney was crystallized by the sitting-drop vapour-diffusion method. The crystal diffracted to 1.80 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of the Bag2 amino-terminal domain from Mus musculus

The amino-terminal domain of the Hsp70 co-chaperone Bag2 from M. musculus has been crystallized in native and selenomethionyl forms diffracting to 2.27 and 3.1 Å resolution, respectively.

Crystallization and preliminary X-ray analysis of the haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58

The haloalkane dehalogenase DatA from A. tumefaciens C58 was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.70 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of the ATPase domain of human TAP in nucleotide-free and ADP-, vanadate- and azide-complexed forms

The ATPase domain of human TAP in nucleotide free, ADP, vanadate and azide complexed forms were purified and crystallized. The X-ray diffraction data sets were collected for all crystals in the resolution range of 2.8–3.0 Å.

Crystallization and preliminary X-ray diffraction of malate de­hydrogenase from Plasmodium falciparum

The collection of X-ray diffraction data from malate dehydrogenase from P. falciparum to a resolution of 2.2 Å is reported.

Crystallization and preliminary crystallographic studies of both components of the staphylococcal LukE–LukD leukotoxin

The expression, purification and crystallization of the soluble forms of LukE and LukD, which together constitute the LukE–LukD staphylococcal leukotoxin, are described.

Crystallization and preliminary X-ray crystallographic analysis of human peptidylarginine deiminase type III

Human peptidylarginine deiminase type III was crystallized using polyethylene glycol monomethylether or polyethylene glycol as a precipitant. The crystals belonged to space group R3, with unit-cell parameters a = b = 114.97, c = 332.49 Å (hexagonal axes), and contained two molecules in an asymmetric unit.

Expression, purification, crystallization and preliminary X-ray analysis of Plasmodium falciparum GTP:AMP phosphotransferase

P. falciparum GTP:AMP phosphotransferase was expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 2.90 Å.

Purification, crystallization and preliminary X-ray analysis of human histidine de­carboxylase

Human histidine decarboxylase was crystallized by the sitting-drop vapour-diffusion method. Diffraction data were collected to 1.8 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of bacterial tRNA^Sec in complex with seryl-tRNA synthetase

Bacterial selenocysteine tRNA was crystallized as the heterologous complex with archaeal seryl-tRNA synthetase. X-ray diffraction was improved by introducing point mutations and heavy-atom labeling, and a 3.2 Å diffraction data set for phase determination was obtained from a platinum-labeled crystal.

Crystallization and preliminary X-ray analysis of the C-terminal domain of CCM2, part of a novel adaptor protein involved in cerebral cavernous malformations

The purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of CCM2, which is part of a signalling complex, are reported.

Overexpression, purification, crystallization and preliminary X-ray analysis of putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv

Rv0864, an enzyme of the Moco biosynthesis pathway from M. tuberculosis, has been overexpressed, purified and crystallized. Diffraction data to 2.5 Å were collected and the structure solved by molecular replacement.

Cloning, purification, crystallization and preliminary X-ray studies of human α₁-microglobulin

Here, the expression, purification, crystallization and preliminary crystallographic analysis of human α₁m are reported.

Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa

MvfRC87, a 242-residue C-­terminal segment of the LysR-type transcriptional regulator MvfR, was produced in Escherichia coli, purified and crystallized.

Crystallization of the novel S-adenosyl-L-me­thionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis

Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-­adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the apo form of InsP₅ 2-K from Arabidopsis thaliana

A mutated version of InsP₅ 2-K allows the production of crystals of the apo form and structure determination using X-ray crystallography.

Purification, crystallization and preliminary X-ray diffraction data of UDP-galacto­pyranose mutase from Aspergillus fumigatus

The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction data are described for UDP-galactopyranose mutase, an enzyme involved in cell-wall synthesis in A. fumigatus.

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of a cyclophilin A-like protein from Piriformospora indica

Cyclophin A like protein from Piriformospora indica involved in salt-stress tolerance was cloned, overexpressed, purified and crystallized. The crystals obtained diffracted X-rays to 2 Å resolution and belonged to space group C222₁.

Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW

T. maritima CheA P3-P4-P5 domains were crystallized in complex with CheW. Low-resolution diffraction data were collected to ∼8 Å using synchrotron X-ray radiation.

Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus

Nonstructural protein 2 from avian infectious bronchitis virus has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.

Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii

The reductase component of p-hydroxyphenylacetate 3-hydroxylase from A. baumannii was overexpressed, purified and crystallized. X-ray diffraction data were collected and processed to 2.3 Å resolution.

The ParE2–PaaA2 toxin–antitoxin complex from Escherichia coli O157 forms a heterodocecamer in solution and in the crystal

Crystals of the complex of E. coli O157 ParE2 and PaaA2 belong to space group P3₁21 or P3₂21 and diffract to 3.8 Å resolution.