Crystallographic analysis of an RNA polymerase σ-subunit fragment complexed with −10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein–ssDNA complexes

Structures of a protein–ssDNA complex suggest a possible approach for engineering crystal contacts by virtue of G-quartet formation.

Structure of isochorismate synthase DhbC from Bacillus anthracis

The crystal structure of B. anthracis isochorismate synthase DhbC, which is involved in the biosynthesis of bacillibactin, was determined at 2.4 Å resolution. It was compared with other chorismate-utilizing enzymes and both structural and bioinformatics analyses were performed. The putative active site was pinpointed.

Structural analysis of DNA–protein complexes regulating the restriction–modification system Esp1396I

Comparison of bound and unbound DNA in protein–DNA co-crystal complexes reveals insights into controller-protein binding and DNA distortion in transcriptional regulation.

Structure of formaldehyde de­hydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD⁺

The crystal structure of NAD⁺-bound FDH from P. aeruginosa was determined to 2.7 Å resolution. The overall structure is very similar to that of P. putida FDH. Preliminary kinetics analysis of the enzyme indicated a glutathione-independent bi-substrate `ping-pong' catalytic mechanism.

The structure of amylosucrase from Deinococcus radiodurans has an unusual open active-site topology

Major conformational changes are reported in the structure of unliganded amylosucrase from D. radiodurans.

Structure of the Aeropyrum pernix L7Ae multifunctional protein and insight into its extreme thermostability

The crystal structure of A. pernix L7Ae is reported, providing insight into the extreme thermostability of this protein.

Structure of zebrafish MO25

The conserved protein MO25 activates STE20 family kinases. The crystal structure of zebrafish MO25 is reported, which is similar to that of human MO25.

Expression, purification and crystallization of the ancestral androgen receptor–DHT complex

The ancestral androgen receptor was crystallized in complex with 5α-dihydrotestosterone (DHT) and a fragment of the transcriptional mediator/intermediary factor 2.

Crystallization studies of the keratin-like domain from Arabidopsis thaliana SEPALLATA 3

The keratin-like domain of the transcription factor SEPALLATA 3 was purified and crystallized. Native data were collected to 2.53 Å resolution from a single crystal belonging to space group P2₁2₁2.

Purification, crystallization and preliminary X-ray diffraction analysis of the 23S rRNA methyltransferase RlmJ from Escherichia coli

The 23S rRNA methyltransferase RlmJ from E. coli has been cloned, expressed, purified and crystallized. X-ray diffraction data to 1.85 Å resolution have been collected from the apo RlmJ crystals.

Crystallization and preliminary X-ray crystallographic analysis of the amylomaltase from Corynebacterium glutamicum

The crystallization and preliminary X-ray analysis of the full-length amylomaltase from C. glutamicum are reported.

Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active β-1,4-D-mannanase from the Antarctic springtail Cryptopygus antarcticus

A cold-active β-1,4-D-mannanase from the Antarctic springtail C. antarcticus (CaMan) has been expressed, purified and crystallized. The CaMan crystal belonged to space group P2₁2₁2₁, with unit-cell parameters a = 73.40, b = 83.81, c = 163.55 Å, and diffracted to 2.35 Å resolution. A 2.40 Å resolution data set was also collected from a CaMan–mannopentaose complex crystal.

Cloning, purification and preliminary crystallographic analysis of the Helicobacter pylori leucyl aminopeptidase–bestatin complex

Leucyl aminopeptidase from H. pylori was purified and crystallized by the hanging-drop vapour-diffusion method. A diffraction data set was collected to 2.8 Å resolution.

Neutron diffraction studies towards deciphering the protonation state of catalytic residues in the bacterial KDN9P phosphatase

A complete neutron data set was collected from a medium-sized H/D-exchanged crystal of KDN9P phosphatase. Initial refinement to 2.3 Å resolution using only neutron data showed significant density for catalytically important residues.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human myosin 1c in complex with calmodulin

Human myosin 1c and its light chain calmodulin were co-expressed and purified to homogeneity from Sf9 cells and crystallized. The crystals belonged to space group P2₁ and diffracted to 3 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of the variable domain of Scl2.3, a streptococcal collagen-like protein from invasive M3-type Streptococcus pyogenes

In this study, the variable domain of the collagen-like protein Scl2 from invasive M3-type S. pyogenes has successfully been crystallized. Single-wavelength anomalous dispersion experiments have been carried out to obtain experimental phases by preparing crystal derivatives with lanthanides. Model building and refinement, which are in progress, will provide the first structural clues for Scls.

Preliminary X-ray crystallographic studies of the TRAF domain of human TRAF4

The human TRAF4 TRAF domain was crystallized and the crystals were found to belong to space group P2₁2₁2₁, with unit-cell parameters a = 58.9, b = 87.9, c = 117.3 Å, α = β = γ = 90°. The crystals were obtained at 293 K and diffracted to a resolution of 2.3 Å.

Cloning, expression, purification and preliminary X-ray analysis of the dimerization domain of ethylene response sensor 1 (ERS1) from Arabidopsis thaliana

The central dimerization domain of the ethylene receptor ERS1 from A. thaliana was expressed, purified and crystallized. The crystals belonged to space groups C222₁ and P2₁2₁2.

Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans

Adenylosuccinate synthetase from C. neoformans has been crystallized and the crystals diffracted to a resolution of 2.2 Å.

Expression, crystallization and preliminary X-ray crystallographic analysis of alcohol de­hydrogenase (ADH) from Kangiella koreensis

Alcohol dehydrogenase (ADH) catalyzes the oxidation of alcohols using NAD(P)⁺ as a cofactor. The adh gene from K. koreensis was cloned and the protein was expressed, purified and crystallized. A preliminary X-ray crystallographic analysis of the ADH crystal was performed.

Expression, crystallization and preliminary X-ray crystallographic analysis of alanine racemase from Acinetobacter baumannii OXA-23

Alanine racemase (ALR) catalyzes the racemization of L-alanine to D-alanine with pyridoxal 5′-phosphate (PLP) as a cofactor. The alr gene from A. baumannii OXA-23 was cloned and the protein was expressed, purified and crystallized. A preliminary X-ray crystallographic analysis of the ALR crystal was performed.

Fibronectin type III domains engineered to bind CD40L: cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of two complexes

Tn3 proteins, a novel class of binding molecules based on a fibronectin type III domain, have been cloned, expressed, purified and crystallized in complex with their cognate target.

Crystallization and preliminary X-ray diffraction analysis of human importin β–Snail zinc finger domain complex

The overproduction, co-purification, crystallization and preliminary X-ray analysis of the complex between importin β and Snail zinc finger domain are described.

Crystallization of the HigBA2 toxin–antitoxin complex from Vibrio cholerae

Crystals of the V. cholerae HigBA2 complex and of the C-terminal domain of HigA2 diffracted to 2.2 and 1.8 Å resolution, respectively.

Identifying ligand-binding hot spots in proteins using brominated fragments

X-ray crystallographic screening can provide both leads for high-affinity small-molecule development and information about potential substrate sites. In this study, in work on the well-studied protein T. thermophilus EF-Tu, brominated fragments were used for easy identification in anomalous maps.

Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

Serial femtosecond X-ray (SFX) diffraction extending beyond 6 Å resolution using T. thermophilus 30S ribosomal subunit crystals is reported.

Expression, purification, crystallization and preliminary X-ray studies of Lactobacillus jensenii enolase. Corrigendum

A correction is made to the article by Harris et al. [(2010) Acta Cryst. F66, 938–940].