Unexpected features in the Protein Data Bank entries 3qd1 and 4i8e: the structural description of the binding of the serine-rich repeat adhesin GspB to host cell carbohydrate receptor is not a solved issue

A recently published crystallographic model of GspB in complex with a disaccharide was proposed to provide insight into the interaction of the S. gordonii adhesin with the cell sialyl-T antigen from host cells. However, a close inspection of this model revealed that in this complex a buffer molecule was mistaken for the disaccharide. As a consequence, this complex provides no information regarding the interaction of GspB with host cell receptors.

A monoclinic crystal form of casein kinase 1 δ

The crystal structure of a new P2₁ crystal form of the catalytic domain of mammalian casein kinase 1 δ is described and compared with previously deposited structures.

Structure of 2-keto-3-de­oxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa

The recombinant production, purification, crystallization and crystal structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from P. aeruginosa is presented.

Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster

The crystal structure of succinyl-CoA: 3-ketoacid CoA transferase from Drosophila melanogaster was determined at 2.64 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

This report describes the crystallization and X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1.

Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7

The 155 amino-acid FP domain of the human F-box protein Fbxo7 was successfully expressed in bacteria, purified and crystallized. Native and single-wavelength anomalous dispersion data sets have been collected.

Preliminary X-ray diffraction analysis of thermostable β-1,4-mannanase from Aspergillus niger BK01

The recombinant protein of acidic thermophilic β-1,4-mannanase from Aspergillus niger BK01 was expressed, purified, and crystallized. X-ray diffraction data to 1.57 Å was collected and reported.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SpyCEP, a candidate antigen for a vaccine against Streptococcus pyogenes

The cloning, expression and purification of different fragments of the S. pyogenes SpyCEP protein are reported. Preliminary X-ray diffraction analysis of a 1580-residue selenomethionine-labelled ectodomain fragment is described.

Purification, crystallization and preliminary crystallographic studies of C-terminal RNA recognition motif (RRM-3) of human ELAV-type RNA-binding protein 3 (ETR-3)

The C-terminal RNA recognition motif of human ETR-3 protein involved in myotonic dystrophy was over-expressed, purified and crystallized. The crystals obtained diffracted X-rays to 3 Å resolution and belonged to space group P2₁3.

Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of Rv0241c (HtdX) from Mycobacterium tuberculosis H37Rv

A putative (3R)-hydroxyacyl-CoA dehydratase, HtdX from M. tuberculosis H37Rv, has been cloned, overexpressed, purified and crystallized to collect preliminary X-ray diffraction data.

Crystallization and preliminary crystallographic analysis of GanB, a GH42 intracellular β-galactosidase from Geobacillus stearothermophilus

The GH42 β-galactosidase GanB from G. stearothermophilus has been crystallized in the primitive orthorhombic space group P2₁2₁2₁. Complete diffraction data sets have been measured for the wild-type enzyme (2.45 Å resolution) and its catalytic mutant (E323A; 2.50 Å resolution) for use in a full three-dimensional structural analysis of the GanB protein.

Expression, crystallization and preliminary X-ray crystallographic analysis of peptide de­formylase from Campylobacter jejuni

Peptide deformylase catalyzes the deformylation of N-formylated methionine in newly synthesized polypeptides in prokaryotes and some eukaryotic organelles. The synthetic codon-optimized def gene from C. jejuni was cloned and the protein expressed, purified and crystallized. A preliminary X-ray crystallography analysis of the C. jejuni peptide deformylase crystals was performed.

Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1

A proteolytically stable fragment of a plasmid replication initiation protein from the thermophile G. stearothermophilus has been biochemically characterized, crystallized and diffraction data collected to a resolution of 2.5 Å.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron

A putative xylose isomerase from B. thetaiotaomicron has been crystallized (space group P1, unit-cell parameters a = 96.3, b = 101.7, c = 108.3 Å, α = 82.8, β = 68.2, γ = 83.0°). Diffraction data have been collected to 2.10 Å resolution using synchrotron X-rays.

Crystallization and preliminary X-ray diffraction studies of D-threo-3-hydroxyaspartate dehydratase isolated from Delftia sp. HT23

Improvement of the expression level, crystallization and preliminary X-ray diffraction studies of D-threo-3-hydroxyaspartate dehydratase isolated from Delftia sp. HT23 are reported.

Crystallization of the C-terminal head domain of the fibre protein from a siadenovirus, turkey adenovirus 3

The C-terminal domain of the fibre protein from turkey adenovirus 3, a siadenovirus, consisting of amino acids 304–454, has been crystallized. Diffraction data were obtained to 2.0 and 2.14 Å resolution for a native crystal and a selenomethionine-derivative crystal, respectively.

Crystallization and preliminary X-ray study of a (2R,3R)-2,3-butanediol de­hydrogenase from Bacillus coagulans 2-6

A (2R,3R)-2,3-butanediol dehydrogenase from B. coagulans 2-6 was expressed in E. coli BL21 (DE3) and purified. Crystallization and preliminary X-ray crystallographic analysis were performed for this recombinant enzyme.

Crystallization and preliminary X-ray diffraction analysis of a high-affinity phosphate-binding protein endowed with phosphatase activity from Pseudomonas aeruginosa PAO1

A phosphate-binding protein endowed with phosphatase activity, previously dubbed LapA, from P. aeruginosa PAO1 has been crystallized. The crystals diffracted to 0.87 Å resolution and belonged to space group P2₁.

Crystallization and preliminary crystallographic analysis of a viral RNA-silencing suppressor encoded by Wuhan nodavirus

A viral RNA-silencing suppressor encoded by Wuhan nodavirus has been overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.

Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72

Diffraction data were collected from a NylC_p2 crystal to a resolution of 1.60 Å. The crystal belonged to space group C222₁, with unit-cell parameters a = 70.84, b = 144.90, c = 129.05 Å.

Expression, purification and preliminary X-ray crystallographic analysis of Arf1-GDP in complex with dimeric p23 peptide

The complex of Arf1-GDP and dimeric p23 peptide, which is likely to be involved in membrane binding of Arf1, has been crystallized and preliminary crystallographic analysis was performed.

Purification, crystallization and preliminary X-ray crystallographic analysis of the transport unit of the monomeric autotransporter AIDA-I from Escherichia coli

Here, the purification, crystallization and preliminary X-ray crystallographic studies of the transport unit of AIDA-I are reported.

Expression, crystallization and preliminary X-ray crystallographic analysis of cellobiose 2-epimerase from Dictyoglomus turgidum DSM 6724

Cellobiose 2-epimerase epimerizes and isomerizes β-1,4- and α-1,4-gluco-oligosaccharides. The cellobiose 2-epimerase gene from D. turgidum was cloned and the protein was expressed, purified and crystallized. A preliminary X-ray crystallography analysis of the cellobiose 2-epimerase crystals was performed.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the TIR domain from the Brucella melitensis TIR-domain-containing protein TcpB

In this study, the B. melitensis TIR-domain-containing protein (TcpB) was cloned, expressed, purified and crystallized, and X-ray diffraction data were collected to 2.6 Å resolution.

Crystallization and preliminary structural characterization of the two actin isoforms of the malaria parasite

The two Plasmodium actin isoforms were expressed, purified and crystallized in complex with the gelsolin G1 domain. Crystals diffracting to high resolution were obtained and data sets for actin I and II were collected to 1.19 and 2.2 Å resolution, respectively.

Cloning to crystallization of di­hydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila

Dihydrodipicolinate synthase (DHDPS) catalyses the rate-limiting step in the biosynthesis of meso-diaminopimelate and lysine. Here, the cloning, expression, purification and crystallization of DHDPS from the intracellular pathogen Legionella pneumophila are described.

Purification, crystallization and preliminary crystallographic analysis of the SpaA backbone-pilin subunit from probiotic Lactobacillus rhamnosus GG

SpaA pilin, which forms the pilus shaft of SpaCBA pili in L. rhamnosus GG, was crystallized and X-ray diffraction data were collected to a resolution of 2.0 Å.

Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant α-cyclodextrin glucanotransferase from Bacillus macerans

The constructed Y167H mutant α-cyclodextrin glucanotransferase was successfully expressed and purified. Single crystals were grown with two kinds of morphology in different crystallization conditions.