Latest methods of fluorescence-based protein crystal identification

Fluorescence, whether intrinsic or by using trace fluorescent labeling, can be a powerful aid in macromolecule crystallization. Its use in screening for crystals is discussed here.

Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein

The preliminary structural analysis of VSR in complex with the C-terminal sorting determinant of a seed storage protein is reported.

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1–CBM22-2 tandem

The P. barcinonensis xylanase 10C N-terminal domain, containing the CBM22-1–CBM22-2 tandem, was overexpressed, purified and crystallized, and its preliminary X-ray diffraction analysis is reported at 2.43 Å resolution.

Expression, purification and preliminary crystallographic analysis of a haem-utilizing protein, HutX, from Vibrio cholerae

HutX from V. cholerae was expressed, purified and crystallized. The crystals were of sufficient quality to collect high-resolution data sets and carry out a preliminary X-ray crystallographic analysis.

Structure of the apo anti-influenza CH65 Fab

The crystal structure of anti-influenza antibody CH65 at 1.70 Å resolution reveals that the affinity-matured antibody evolved to preconfigure the antibody loops to increase binding affinity to the hemagglutinin.

Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway

VioD, a hydroxylase in the violacein-biosynthesis pathway, was crystallized and a complete 1.7 Å resolution data set was collected. The crystal belonged to space group P3₁, with unit-cell parameters a = b = 90.0, c = 94.5 Å, α = β = 90, γ = 120°.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of tomato β-galactosidase 4

Tomato β-galactosidase 4 has been expressed in P. pastoris, purified and crystallized. Diffraction data have been collected to a resolution of 1.65 Å.

The role of flexibility and molecular shape in the crystallization of proteins by surface mutagenesis

The role of surface flexibility and molecular shape in protein crystallization is investigated.

Structural analysis of ibuprofen binding to human adipocyte fatty-acid binding protein (FABP4)

The ligand-binding properties of human adipocyte fatty-acid binding protein is crystallographically probed with a set of compounds structurally related to ibuprofen.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the effector-interaction domain of the resistance protein RGA5-A from Oryza sativa L. japonica

RGA5-A_S, the effector-interaction domain of the resistance protein RGA5-A, was expressed, purified and crystallized, yielding crystals that diffracted to 2.43 Å resolution.

Structural basis for the recognition of the scaffold protein Frmpd4/Preso1 by the TPR domain of the adaptor protein LGN

The crystal structure of the TPR domain of LGN in complex with the LGN binding region of Frmpd4/Preso1 is presented at 1.5–Å resolution. Comparison with structures of LGN complexed with its other partners has identified the consensus sequence motif (E/Q)XEX_4–5(E/D/Q)X_1–2(K/R)X_0–1(V/I) as the common core LGN-binding region.

Preliminary X-ray crystallographic analysis of an engineered variant of human chimera-type galectin-3 with a shortened N-terminal domain

A variant of human galectin-3 containing sections of the N-terminal domain has been crystallized for the first time. The crystals belonged to the orthorhombic space group P2₁2₁2₁ and diffracted to 3.3 Å resolution.

Crystallization and preliminary crystallographic analysis of the putative sugar-binding protein Msmeg_0515 (AgaE) from Mycobacterium smegmatis

The M. smegmatis gene Msmeg_0515 encoding the AgaE protein has been cloned, expressed in E. coli and purified. Crystals of AgaE have been grown that diffracted to beyond 1.5 Å resolution and belonged to space group P2₁2₁2₁ with a single AgaE polypeptide in the asymmetric unit.

Crystallization and preliminary X-ray crystallographic analysis of Z-ring-associated protein (ZapD) from Escherichia coli

The E. coli ZapD protein was expressed in E. coli, purified and crystallized using lithium sulfate as a precipitant. X-ray diffraction data were collected to 2.95 Å resolution.

Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate

The crystal structure of a substrate-trapping mutant of human dual-specificity phosphatase 22 (DUSP22) in complex with the small molecule substrate 4-nitrophenyl phosphate was determined at 1.34 Å resolution.

Crystallization and preliminary X-ray crystallographic studies of dipeptidyl peptidase 11 from Porphyromonas gingivalis

Dipeptidyl peptidase 11 from P. gingivalis (PgDPP11) was crystallized. X-ray diffraction data were collected to 1.82 Å resolution.

Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL)

A periplasmic sensory domain of the C. jejuni chemoreceptor for multiple ligands (CcmL) has been purified and co-crystallized with isoleucine by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 1.3 Å resolution.

Crystallization and preliminary X-ray diffraction studies of the family 54 carbohydrate-binding module from laminarinase (β-1,3-glucanase) Lic16A of Clostridium thermocellum

The paper describes the purification, crystallization and preliminary structural analysis of ctCBM54.

Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena

SM80.1, a 7S vicilin from S. melongena, was identified, purified and crystallized. The crystals belonged to space group P6₃22 and diffracted to 2.21 Å resolution.

Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin–antitoxin module

The HigA2 antitoxin and the HigBA2 toxin–antitoxin complex from V. cholerae were crystallized in complex with their operator box.

Recombinant expression, purification, crystallization and preliminary X-ray diffraction analysis of Haemophilus influenzae BamD and BamCD complex

In this study, the recombinant expression, purification, crystallization and preliminary X-ray diffraction analysis of Haemophilus influenzae BamD and BamCD complex are reported.

Crystallization and preliminary X-ray diffraction analysis of a putative carbon–carbon bond hydrolase from Mycobacterium abscessus 103

The crystallization and preliminary X-ray diffraction analysis of a putative carbon–carbon bond hydrolase from M. abscessus 103 are reported.

Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae

A soluble variant of the monoglyceride lipase Yju3p was successfully expressed, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.