Origin and use of crystallization phase diagrams

While crystallization phase diagrams are excellent tools to conceptualize phase relations in protein solutions, they need to be used appropriately and to be compatible with their physicochemical meaning.

Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1

Human myotubularin-related protein 1 (MTMR1) was crystallized using polyethylene glycol 20 000 as a precipitant. Diffraction data have been collected to 2.0 Å resolution using synchrotron X-rays.

Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody

Crystals of a complex between a fragment of TssM obtained by limited proteolysis and a nanobody have been obtained and diffracted to 1.9 Å resolution.

Purification, crystallization and preliminary X-ray diffraction studies of the β-catenin homolog HMP-2 from Caenorhabditis elegans

The purification, crystallization and molecular-replacement structure solution of two crystal forms of the β-catenin homolog HMP-2 from C. elegans are described.

Structure of the dodecamer of the aminopeptidase APDkam598 from the archaeon Desulfurococcus kamchatkensis

The structure of the dodecamer of the aminopeptidase APDkam598 from the archaeon D. kamchatkensis was determined using X-ray diffraction data to a resolution of 3.0 Å and single-particle electron microscopy.

Structures of Escherichia coli tryptophanase in holo and `semi-holo' forms

Two slightly different crystal forms of E. coli tryptophanase are presented. The transition between apo and holo forms of the enzyme is accompanied by a significant domain shift.

Crystallization and preliminary X-ray diffraction analysis of the arginine repressor ArgR from Bacillus halodurans

Cubic crystals of ArgR from B. halodurans have been obtained. Their diffraction was improved by the dehydration method. X-ray data have been collected to 2.35 Å resolution using synchrotron radiation.

Cwp84, a Clostridium difficile cysteine protease, exhibits conformational flexibility in the absence of its propeptide

Two structures of Cwp84, a cysteine protease from the S-layer of C. difficile, are presented after propeptide cleavage. They reveal the movement of three loops, two in the active-site groove and one on the surface of the lectin-like domain, exposing a hydrophobic pocket.

Retracted: Mode of binding of the antithyroid drug propylthiouracil to mammalian haem peroxidases

The crystal structure of the complex of lactoperoxidase with the antithyroid drug propylthiouracil (PTU) was determined at 2.50 Å resolution. It shows that PTU binds to lactoperoxidase in the substrate-binding site on the distal haem side. The S atom of PTU is coordinated to the haem iron, while the methyl group of the propyl moiety forms van der Waals contacts with the side chain of Ala114.

Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module from family 64 (StX)

A carbohydrate-binding module from S. thermophile belonging to family GH64 was crystallized and diffracted to 1.8 Å. The phases were found using the single-wavelength anomalous diffraction method.

The structure of C290A:C393A Aurora A provides structural insights into kinase regulation

A variant of the Aurora A kinase domain in which both surface cysteines are mutated to alanine generates crystals that diffract to higher resolution than the equivalent crystals produced using wild-type protein. The structure confirms that single phosphorylation on Thr288 is insufficient to stabilize a conformation of the activation loop in a `fully active' state.

Crystallization and preliminary crystallographic analysis of aceto­phenone reductase from Geotrichum candidum NBRC 4597

Acetophenone reductase from G. candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method. The crystal diffracted to 2.6 Å resolution.

Bacterial expression and preliminary crystallographic studies of a 149-residue fragment of human Caprin-1

A protein construct consisting of a 149-residue fragment of human Caprin-1 was successfully expressed in E. coli, purified and crystallized. Native and Se-SAD data sets were collected to resolutions of 2.05 and 2.65 Å, respectively.

Protein production, crystallization and preliminary X-ray analysis of two isoforms of the Dscam1 Ig7 domain

X-ray data were collected for two Dscam1 Ig7 isoforms and were processed to resolutions of 1.95 and 2.37 Å, respectively. Comparison of different Ig7 isoforms will provide insights into the mechanism of its homophilic binding specificity.

Crystallization and preliminary X-ray analysis of the Plasmodium falciparum apicoplast DNA polymerase

P. falciparum apicoplast DNA polymerase has been expressed, purified and crystallized in the apo form.

High-resolution crystal structure of a polyextreme GH43 glycosidase from Halothermothrix orenii with α-L-arabino­furanosidase activity

The crystal structure of the H. orenii glycosidase was determined by molecular replacement and refined at 1.10 Å resolution.

Improved crystallization and diffraction of caffeine-induced death suppressor protein 1 (Cid1)

The use of truncation and RNA-binding mutations of caffeine induced death suppressor protein 1 (Cid1) as a means to enhance crystallogenesis leading to an improvement of X-ray diffraction resolution by 1.5 Å is reported.

Crystallization and preliminary X-ray analysis of Rv1674c from Mycobacterium tuberculosis

Rv1674c from M. tuberculosis, a possible DNA-binding sulfurtransferase, was purified and crystallized. Preliminary crystallographic analysis was performed.