The state-of-the art in macromolecular crystallization: the IYCr series concludes

The IYCr crystallization series comes to an end.

Cloning, expression, purification, crystallization and X-ray diffraction analysis of di­hydrodipicolinate synthase from the human pathogenic bacterium Bartonella henselae strain Houston-1 at 2.1 Å resolution

The enzyme dihydrodipicolinate synthase catalyzes the committed step in the synthetic pathway for- the intermediate diaminopimelate and the essential amino acid lysine in bacteria and plants. Here, the crystallization and X-ray diffraction analysis of dihydrodipicolinate synthase from the human pathogenic bacterium B. henselae, the causative bacterium of cat-scratch disease, are presented.

Structure of a thermostable serralysin from Serratia sp. FS14 at 1.1 Å resolution

The crystal structure of a thermostable serralysin from Serratia sp. FS14 was solved at 1.10 Å resolution.

Expression, crystallization and structure elucidation of γ-terpinene synthase from Thymus vulgaris

γ-Terpinene synthase from T. vulgaris was characterized. The crystal structure was elucidated to almost 1.5 Å resolution.

Crystallization of nepenthesin I using a low-pH crystallization screen

The aspartic protease from N. gracilis was crystallized in complex with the inhibitor pepstatin A using a newly formulated low-pH screen. X-ray diffraction data showed that the crystals diffracted to 2.8 Å resolution.

New crystal form of human ubiquitin in the presence of magnesium

Ubiquitin is a signalling protein that has been reported to crystallize in the presence of metal ions. Here, the first crystallographic structure of human ubiquitin in the presence of magnesium is reported.

Purification, crystallization and structural elucidation of D-galactaro-1,4-lactone cycloisomerase from Agrobacterium tumefaciens involved in pectin degradation

The structural elucidation of a unique lactone cycloisomerase involved in pectin degradation is reported.

Crystal structure of HINT from Helicobacter pylori

Crystallographic analysis of the histidine triad nucleotide protein (HINT) from H. pylori is reported.

Expression, purification, crystallization and crystallographic analysis of the N-terminal domain of translocated intimin receptor

The N-terminal domain of translocated intimin receptor (Tir-N) plays a key role in pathogenic E. coli infection. Tir-N was cloned, expressed, purified and crystallized.

Improved purification, crystallization and crystallographic study of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77

The hydrogenase unit of the Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77 was purified using an improved procedure and was then crystallized. Diffraction data sets from crystals of the samples with and without trypsin treatment were collected at resolutions of 1.60 and 2.00 Å, respectively.

Sqt1p is an eight-bladed WD40 protein

The crystal structure of Sqt1p is presented and its biophysical properties are explored.

Structure of me­thionine γ-lyase from Clostridium sporogenes

The crystal structure of pyridoxal 5′-phosphate-dependent methionine γ-lyase from C. sporogenes was solved at 2.37 Å resolution. The cofactor- and substrate-binding sites and the oligomeric organization of the enzyme are described.