Co-crystal structures of the protein kinase haspin with bis­ubstrate inhibitors

Two high-resolution co-crystal structures of the mitotic protein kinase haspin with previously reported conjugates consisting of an ATP analogue and a histone H3(1–7) peptide analogue confirm the bisubstrate character of the compounds and provide directions for the future development of more specific haspin inhibitors.

Structures of endo­thiapepsin–fragment complexes from crystallographic fragment screening using a novel, diverse and affordable 96-compound fragment library

A novel and affordable fragment library was assembled and applied to the model protease endothiapepsin in a crystallographic fragment-screening experiment.

The Myb domain of LUX ARRHYTHMO in complex with DNA: expression, purification and crystallization

The expression, purification and crystallization of the DNA-binding Myb domain of LUX ARRHYTHMO from A. thaliana in complex with DNA are reported. The binding affinity of the Myb domain for DNA was measured and was found to be in the low-nanomolar range, suggesting that a single Myb domain is able to bind to its cognate DNA with very high affinity.

Structural analysis of a phosphonate hydroxylase with an access tunnel at the back of the active site

The crystal structure of the dioxygenase FrbJ is reported. An access tunnel was found at the back of the active site, which connects the putative binding site for α-ketoglutarate to the solvent.

Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP⁺

The crystal structure of ketopantoate reductase complexed with NADP⁺ was determined at 2.3 Å resolution.

Crystal structure of bile salt hydrolase from Lactobacillus salivarius

Bile salt hydrolase is an enzyme that is produced by various commensal bacteria in the intestine and is also a key mechanistic microbiome target for enhanced animal and human health.

The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis

The periplasmic sensing domain of V. fischeri chemoreceptor protein A was purified and crystallized. Analysis of an X-ray diffraction data set collected to 1.8 Å resolution is presented.

Structural and functional insights into the stationary-phase survival protein SurE, an important virulence factor of Brucella abortus

A crystallographic analysis of the stationary-phase survival protein from B. abortus is reported.

RRM domain of human RBM7: purification, crystallization and structure determination

The X-ray crystal structure of the RRM domain of human RBM7 reveals a pentameric assembly.

1.2 Å resolution crystal structure of the periplasmic aminotransferase PvdN from Pseudomonas aeruginosa

PvdN (PA2394) from P. aeruginosa is a nonribosomal peptide synthetase tailoring enzyme that is involved in pyoverdine biosynthesis. PvdN has been identified as a periplasmic protein that is pyridoxal 5′-phosphate-dependent and shows homology to class I aminotransferases. Here, the crystal structure of PvdN is reported at 1.22 Å resolution.

Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum

The crystal structure of Prp28 from C. thermophilum is reported at 3.2 Å resolution. This DEAD-box helicase exhibits multiple functions during spliceosome assembly.