Crystal structures of a yeast 14-3-3 protein from Lachancea thermotolerans in the unliganded form and bound to a human lipid kinase PI4KB-derived peptide reveal high evolutionary conservation

Crystal structures of a 14-3-3 protein (Bmh1) from the yeast L. thermotolerans in the unliganded form and in complex with a peptide from a human homologue (PI4KB) of its binding partner Pik1 reveal the high evolutionary conservation of 14-3-3 proteins. Indeed, the yeast 14-3-3 protein binds its human-derived ligand in a manner highly similar to that of human 14-3-3 proteins.

Crystal structure of a phosphoribosyl anthranilate isomerase from the hyperthermophilic archaeon Thermococcus kodakaraensis

The crystal structure of a phosphoribosyl anthranilate isomerase from the hyperthermophilic archaeon T. kodakaraensis was determined in space groups P1 and C2 at 1.75 and 1.85 Å resolution, respectively.

The structure of the metallo-β-lactamase VIM-2 in complex with a triazolylthioacetamide inhibitor

The crystal structure of a triazolylthioacetamide inhibitor in complex with Verona integron-encoded metallo-β-lactamase 2 (VIM-2) is reported at a resolution of 1.50 Å. The structure shows that the inhibitor binds to the active site of the enzyme and reveals detailed information on the inhibitor interactions.

Natural and non-natural amino-acid side-chain substitutions: affinity and diffraction studies of meditope–Fab complexes

The structure–affinity relationship of meditope–cetuximab complexes is investigated by measuring their affinity using surface plasmon resonance and determining their structures using X-ray crystallography.

Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12

The 1.34 Å resolution crystal structure of fuculose aldolase from the psychrophilic yeast G. antarctica reveals characteristics of the enzymatic site and tolerance to cold temperature.

Development of a high-throughput crystal structure-determination platform for JAK1 using a novel metal-chelator soaking system

Active JAK1 kinase domain was crystallized with MgADP and soaked in the presence of EDTA to allow displacement of the nucleotide by compounds of interest. This high-throughput method of structure determination may be widely applicable to other protein kinases and small-molecule ATP-competitive ligands.

Structural characterization of the thermostable Bradyrhizobium japonicum D-sorbitol de­hydrogenase

B. japonicum D-sorbitol dehydrogenase is NADH-dependent. It is of interest for industrial applications because of its ability to also oxidize L-glucitol and its thermostability. Here, its structure is presented and discussed.