Crystal structure of yeast mono­thiol glutaredoxin Grx6 in complex with a gluta­thione-coordinated [2Fe–2S] cluster

The dimeric structure of the C-terminal domain of Grx6, bridged by one [2Fe–2S] cluster coordinated by the active-site Cys136 and two external glutathione molecules, is reported.

Arabidopsis receptor-like cytoplasmic kinase BIK1: purification, crystallization and X-ray diffraction analysis

The purification, crystallization and X-ray diffraction analysis of the Arabidopsis receptor-like cytoplasmic kinase BIK1, which plays an important role in growth and immune signaling pathways, are described.

Crystal and solution structural studies of mouse phospholipid hydroperoxide gluta­thione peroxidase 4

The crystal structure of mouse phospholipid hydroperoxide glutathione peroxidase 4 solved at 1.8 Å resolution and the first solution structural studies of a glutathione peroxidase protein are reported.

The structure of a glycoside hydrolase 29 family member from a rumen bacterium reveals unique, dual carbohydrate-binding domains

A high-resolution crystal structure of an unknown glycoside hydrolase enzyme from the rumen of B. taurus is presented. Unique dual carbohydrate-binding domains are revealed.

Comparative analysis of anti-polyglutamine Fab crystals grown on Earth and in microgravity

Microgravity was used in an attempt to define the crystal structure of the polyQ repeat of huntingtin alone or bound to MW1, an anti-polyQ Fab. While huntingtin was not crystallized in the experiments, analysis of microgravity-grown and Earth-grown MW1 Fab crystals showed that, on average, microgravity-grown crystals of MW1 Fab showed an increase in size and an improvement in resolution and mosaicity when compared with Earth-grown crystals in one space group, in agreement with data published for other proteins, although the highest overall resolution X-ray data in our experiments were obtained from a crystal grown on Earth.

Crystal structure of rofecoxib bound to human cyclooxygenase-2

New crystallization conditions were identified that allowed the structure determination of human cyclooxygenase-2 in complex with rofecoxib (Vioxx) and the structure was subsequently determined to 2.7 Å resolution.

Crystallographic study of the 2-thioribothymidine-synthetic complex TtuA–TtuB from Thermus thermophilus

TtuA and TtuB are the sulfur transferase and sulfur carriers for the biosynthesis of 2-thioribothymidine in some bacterial tRNAs. To elucidate their mechanism of interaction, the TtuA–TtuB complex from T. thermophilus was crystallized and a Zn-MAD data set was collected to a resolution of 2.5 Å.

X-ray crystallographic studies of the extracellular domain of the first plant ATP receptor, DORN1, and the orthologous protein from Camelina sativa

The extracellular domain of the A. thaliana ATP receptor DORN1 has been expressed and purified; a protein orthologous to DORN1 from C. sativa has been crystallized and its X-ray diffraction data have been collected.

Biochemical, spectroscopic and X-ray structural analysis of deuterated multicopper oxidase CueO prepared from a new expression construct for neutron crystallography

Crystals of the multicopper oxidase CueO obtained from a perdeuterated sample for neutron diffraction diffracted to 1.8 Å resolution using X-rays and their crystal structure was solved.

Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyro­phosphorylase VTC1

The GDP-D-mannose pyrophosphorylase VTC1 from A. thaliana was crystallized and co-crystallized with its substrate. X-ray diffraction data were collected for crystallographic analysis.