issue contents

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X

May 2017 issue

Highlighted illustration

Cover illustration: Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4 Å resolution (Karthik et al., p. 259).

research communications


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The structure of a binary complex of NADP+ with 7β-hydroxysteroid dehydrogenase reveals two modes of cofactor binding.

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LsrK from E. coli phosphorylates imported AI-2 molecules and triggers quorum-sensing signalling. The protein was expressed by applying heat and osmotic shock before IPTG induction, and a crystal of LsrK diffracted to 2.9 Å resolution.

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The structure of the tetradecamer sequence d(CCGGGGTACCCCGG)2 is reported here as an A-DNA duplex at 1.4 Å resolution.

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The structures of inactive mutants (F106V and L348M) of the cytochrome P450 vitamin D3 hydroxylase were determined at 1.95 and 2.7 Å resolution, respectively.

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The RNA 2′,3′-cyclic phosphodiesterase (CPDase) from D. radiodurans was crystallized and the crystals diffracted to 1.6 Å resolution. The structure, with an open active site, reveals an elegant mechanism for a CPDase enzyme, suggesting a conserved mechanism of catalysis.

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The response regulator Rv0081 is an essential regulatory hub for promoting the long-term survival of M. tuberculosis. Here, the production, crystallization and initial X-ray crystallographic analysis of Rv0081 are reported.

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The structures of four nanobodies raised against the periplasmic domain of PorM were solved, and their interaction with their target was analysed. Diffracting crystals of two PorM constructs in complex with their specific nanobodies were obtained.

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The crystal structure of human CD27 was determined at a resolution of 1.8 Å, revealing the assembly of the cysteine-rich domains. The binding of the antibody at the edge of the receptor molecule is unusual for the tumor necrosis factor receptor superfamily.

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An archaeal lectin from Methanococcus voltae A3 has been cloned, expressed, purified and crystallized, and the crystals have been characterized using X-ray diffraction.
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