issue contents
September 2017 issue
research communications
The interaction of DAPI with d(CGTGAATTCACG) results in displacement of the ordered spine waters and confers hydrophobic behaviour on the DNA.
PDB reference: d(CGTGAATTCACG) with DAPI, 5t4w
Two forms of the HicAB toxin–antitoxin complex from E. coli were purified and crystals were obtained of one of the forms.
3,6-Anhydro-L-galactonate cycloisomerase (ACI) is a second-step enzyme in the metabolic pathway of 3,6-anhydro-L-galactose. Structural analysis of ACI is crucial in order to elucidate its function, substrate specificity and reaction mechanism at the molecular level.
The crystal structure of zearalenone hydrolase from C. batiana is reported.
PDB reference: lactonase from Cladophialophora bantiana, 5xwz
The Roco protein from the bacterium C. tepidum has been used as a model system to investigate the structure and mechanism of the Roco protein family. Here, the crystallization and crystallographic analysis of the LRR-Roc-COR construct of the C. tepidum Roco protein are reported.
Crystals of PsbO were grown in capillaries using an agarose-plug setup and were cross-linked using glutaraldehyde. Initial neutron diffraction data were collected to 2.3 Å resolution on the MaNDi instrument at the Spallation Neutron Source.
Plasmodesmata-localized protein 5 (PDLP5) is a member of the cysteine-rich receptor-like plasmodesmata-localized proteins and is involved in the regulation of plasmodesmata permeability. The ectodomain of PDLP5, which possesses two DUF26 domains, was expressed, purified and crystallized by the hanging-drop vapour-diffusion method.
Mycobacterial glucosylglycerate hydrolase is a highly conserved enzyme that is involved in recovery from nitrogen starvation. Here, the production, crystallization and structure solution of Mycobacterium hassiacum glucosylglycerate hydrolase using two-wavelength anomalous diffraction of selenomethionine-substituted crystals are presented.