Crystal structure of the mouse innate immunity factor bacterial permeability-increasing family member A1

The crystal structure of mouse BPIFA1 was solved at 2.5 Å resolution and compared with those of structural homologs.

A cryoprotectant induces conformational change in glyceraldehyde-3-phosphate de­hydrogenase

Trehalose is used in the cryoprotection of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) crystals and induces conformational changes in GAPDH from E. coli. The conformational changes were independent of the duration of cryoprotectant soaking for up to 10 min.

The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies

Siderophore-interacting proteins (SIPs) can reduce ferric iron to ferrous iron and cause iron to be released from the ferric siderophore complex. The SIP from Vibrio anguillarum 775 has been expressed, purified and crystallized. The crystal diffracted to 1.113 Å resolution, which is the highest resolution so far observed for a member of the SIP family.

Crystal structures of human CK2α2 in new crystal forms arising from a subtle difference in salt concentration

New crystal forms of human CK2α2 were discovered and one showed improved crystallographic resolution to a maximum of 1.89 Å.

Crystal structure of chorismate mutase from Burkholderia thailandensis

The recombinant production and crystal structure of chorismate mutase from Burkholderi thailandensis, which is a proxy for more virulent Burkholderia species, are presented. The enzyme shares conserved binding-cavity residues with other chorismate mutases, including those with which it has no appreciable sequence identity.

In situ proteolysis of an N-terminal His tag with thrombin improves the diffraction quality of human aldo-keto reductase 1C3 crystals

In situ specific proteolytic removal of a His tag by thrombin improves the morphology and diffraction quality of crystals of human aldo-keto reductase 1C3.

Improved protein-crystal identification by using 2,2,2-tri­chloro­ethanol as a fluorescence enhancer

Protein-crystal labelling with 2,2,2-trichloroethanol for more sensitive detection using UV light is described.

Structure of the tandem PX-PH domains of Bem3 from Saccharomyces cerevisiae

The structure of the putative membrane-binding tandem PX-PH domain module of the yeast protein Bem3 is reported.

The CD163 long-range scavenger receptor cysteine-rich repeat: expression, purification and X-ray crystallographic characterization

The CD163 long-range scavenger receptor cysteine-rich (SRCR) repeat was prepared in a Drosophila system and was crystallized using 20% PEG 4000, 0.15 M potassium sodium tartrate tetrahydrate pH 8.5. X-ray crystallographic characterization of this long-range SRCR repeat will provide structural and functional information for the scavenger receptor superfamily.