Structural polymorphism of the Escherichia coli poly-α-L-glutamate synthetase RimK

Two different conformations, closed and open forms, of the E. coli poly-α-L-glutamate synthetase protein RimK were determined in complex with the ATP analogue AMP-PNP by X-ray crystallography at 2.05 Å resolution. The structure of the open form of RimK is unique and differs substantially from the structures of its homologues.

Crystallization and X-ray analysis of all of the players in the autoregulation of the ataRT toxin–antitoxin system

The crystallization and structural analysis of the various players in the ataRT toxin–antitoxin module from enteropathogenic Escherichia coli are reported: the toxin AtaT, the intrinsically disordered antitoxin AtaR and its complex with a 22 bp fragment of the operator DNA.

Crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with a­cyclovir

The three-dimensional structure of Escherichia coli purine nucleoside phosphorylase (PNP) complexed with acyclovir has been solved and a comparison with other complexes of PNP with guanosine derivatives has been carried out.

Cinder: keeping crystallographers app-y

A mobile application has been developed to facilitate the human annotation of crystallization images.

A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans: cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis

The gene encoding a novel bacterial class V dye-decolourizing peroxidase (DyP) from the extremely radiation-resistant and desiccation-resistant bacterium Deinococcus radiodurans was cloned and optimum conditions for high-yield expression were identified. The optimized crystals diffracted to 2.2 Å resolution and the initial characterization and crystallographic analysis is promising with respect to providing highly desired insights into this largely unknown family of DyPs.

Crystal structure of human anterior gradient protein 3

The recombinant expression, purification, crystallization and crystal structure of human anterior gradient protein 3, a single-domain member of the protein disulfide isomerase family that lacks the canonical active-site motif, are described.

Crystal structures and kinetic analyses of N-acetylmannosamine-6-phosphate 2-epimerases from Fusobacterium nucleatum and Vibrio cholerae

Structure–function studies on N-acetylmannosamine-6-phosphate 2-epimerases from F. nucleatum and V. cholerae reveal conserved features among this class of epimerases.