Structure of Aspergillus aculeatus β-1,4-galactanase in complex with galactobiose

The structure of Aspergillus aculeatus β-1,4-galactanase, a glycoside hydrolase belonging to family 53, has been determined in complex with galactobiose at subsites −1 and −2.

Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan

Crystal structures of the extracellular domain of rabbit neprilysin and of neprilysin complexed with the inhibitors phosphoramidon or thiorphan are reported.

Crystal structure of the SPRY domain of human SPSB2 in the apo state

The apo structure of the SPRY domain of human SPRY domain-containing SOCS box protein 2 (SPSB2) determined at 1.9 Å resolution shows that its inducible nitric oxide synthase (iNOS)-binding site is highly preformed and that the C-terminal His₆ tag binds to a shallow pocket adjacent to the iNOS-binding site, which may help in structure-based and fragment-based SPSB2 inhibitor design.

Cruzain structures: apocruzain and cruzain bound to S-methyl thio­methane­sulfonate and implications for drug design

High-resolution structures of apocruzain and cruzain bound to S-methyl thiomethanesulfonate provide insights for the structure-based design of new cruzain inhibitors.

Structure of the Prx6-subfamily 1-Cys peroxiredoxin from Sulfolobus islandicus

The crystal structure of the 1-Cys peroxiredoxin from S. islandicus is presented; it assembles into a ring-shaped decamer composed of five homodimers. It is concluded that this Prx6-like protein undergoes decamerization independently of arm-domain cysteines.

Crystallization of a potassium ion channel and X-ray and neutron data collection

The growth of a large crystal of a potassium ion channel, and neutron and X-ray data collection from the crystal are described.

A new crystal structure and small-angle X-ray scattering analysis of the homodimer of human SFPQ

The dimerization domain of splicing factor proline/glutamine-rich (SFPQ), an essential RNA-binding protein, has been crystallized in the C-centered orthorhombic space group C222₁ with one monomer in the asymmetric unit, and its structure has been determined and refined to 1.9 Å resolution. The crystal structure was analyzed and compared with the solution scattering data.

Crystal structure of the flavin-dependent thymidylate synthase Thy1 from Thermus thermophilus with an extra C-terminal domain

The crystal structure of the flavin-dependent thymidylate synthase Thy1 from Thermus thermophilus HB8 (TtThy1, TTHA1096) was determined in complex with FAD and phosphate at 2.5 Å resolution. TtThy1 is a tetrameric molecule, to which four FAD molecules are bound. Two phosphate ions were bound to each dUMP-binding site. The characteristic feature of TtThy1 is the existence of an extra C-terminal domain (CTD) consisting of three α-helices and a β-strand.

Structural analysis of the HIN1 domain of interferon-inducible protein 204

Interferon-inducible protein 204 (p204) binds to microbial DNA to elicit inflammatory responses and induce interferon production. The DNA-binding affinity of the p204 HIN1 domain has been characterized and its crystal structure has been determined. Surface-charge distribution together with sequence alignment suggests that the p204 HIN domain uses its L12 and L45 loops for DNA binding.

Changes in the allosteric site of human liver pyruvate kinase upon activator binding include the breakage of an intersubunit cation–π bond

Human liver pyruvate kinase converts phosphoenolpyruvate to pyruvate in the final step of glycolysis and is allosterically activated by fructose-1,6-bisphosphate. The allosteric site is located between the phosphate-binding loop and the allosteric loop. The crystal structures of four site-directed mutants revealed a conformational toggle between the open and closed positions of the allosteric loop.