The structure of MP-4 from Mucuna pruriens at 2.22 Å resolution

The crystal structure of MP-4 from Mucuna pruriens was determined with improved resolution and the recently available gene sequence enabled further analysis of the structure.

Structure of the AAVhu.37 capsid by cryoelectron microscopy

The structure of the capsid of the gene-therapy vector AAVhu.37 was obtained at 2.56 Å resolution by single-particle cryo-electron microscopy.

Conformational flexibility within the small domain of human serine racemase

Serine racemase (SR) is a PLP-dependent enzyme that converts L-serine to D-serine. A 1.9 Å resolution crystal structure of the human SR holoenzyme with two point mutations (Cys2Asp, Cys6Asp) is presented and secondary-structure and tertiary-structure elements are analysed to redefine domain boundaries, structural arrangement and subdomain movement within the small domain by comparison with other holo and ligand-bound SR structures.

The thermodynamic profile and molecular interactions of a C(9)-cytisine derivative-binding acetylcholine-binding protein from Aplysia californica

The binding of a C(9)-cytisine derivative carrying a 3-(hydroxypropyl) modification to acetylcholine-binding protein was characterized using isothermal titration calorimetry and crystallography.

An unexpected co-crystal structure of the calpain PEF(S) domain with Hfq reveals a potential chaperone function of Hfq

A co-crystal structure of the calpain PEF(S) domain bound to the Escherichia coli chaperone Hfq provides insight into the pitfalls of expression of the full-length heterodimeric calpain I complex.

Crystal structures of the cell-division protein FtsZ from Klebsiella pneumoniae and Escherichia coli

In the presence of GTP, the tubulin-like GTPase FtsZ polymerizes into filamentous structures, which are key to cell division. The enzymatic domains of FtsZ from Klebsiella pneumoniae (KpFtsZ) and Escherichia coli (EcFtsZ) formed straight protofilaments in crystals, and both structures adopted relaxed conformations. The T3 loop adopted a unique open conformation in KpFtsZ, while the T3 loop of EcFtsZ was partially disordered.

High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP

Escherichia coli has served as the main model system for examining cell division in bacteria; however, the structure of the central cytokinesis protein FtsZ has not been determined to date. Here, high-resolution structures of E. coli FtsZ in GDP-bound and GTP-bound states are described.