Structural characterization of hexameric shell proteins from two types of choline-utilization bacterial microcompartments

Several crystal structures are analyzed of proteins that form the shells of bacterial microcompartment organelles. These new structures are from microcompartments where the metabolic substrate choline diffuses across the shell-protein pore.

Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site

The Yersinia pestis substrate-binding protein YfeA contains two polyspecific metal-binding sites, and site 2 is capable of collaborating with other protein molecules for inter-protein metal coordination. The inter-protein metal coordination can occur by multiple coordination geometries and may be flexible enough to enable metal transfer to multiple metal-binding proteins.

Complex structure of the acyltransferase VinK and the carrier protein VinL with a pantetheine cross-linking probe

Protein–protein interaction between the acyltransferase VinK and the carrier protein VinL is critical for substrate transfer in vicenistatin biosynthesis. The crystal structure of the VinK–VinL covalent complex formed with a pantetheine cross-linking probe has been determined.

Glycogen phosphorylase revisited: extending the resolution of the R- and T-state structures of the free enzyme and in complex with allosteric activators

Crystal structures of glycogen phosphorylase in the free T-state and R-state and in the R-state in complex with the allosteric activators IMP and AMP are reported at improved resolution.

Biochemical and biophysical characterization of the OXA-48-like carbapenemase OXA-436

Possible mechanisms are demonstrated to explain why the antibiotic-resistance enzyme OXA-436 has higher catalytic rates towards last-resort antibiotics under physiological conditions compared with its more widespread homolog OXA-48.

Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline

A novel device has been designed that could be used as both an in situ macroplate and in situ microplate for high-throughput crystal screening and in situ data collection.