Crystal structure of carbon–nitrogen hydrolase from Helicobacter pylori G27

H. pylori carbon–nitrogen hydrolase was produced and crystallized, and its 2.1 Å resolution structure is reported.

Crystallization and X-ray structure of a highly aggregation-prone monobody engineered for high-affinity small-molecule recognition

This report describes the crystallization and X-ray structure of a highly aggregation-prone monobody engineered for small-molecule recognition. By employing a maltose-binding protein fusion strategy with optimized inter-domain linkers, we successfully obtained diffraction-quality crystals of the protein–ligand complex and determined its structure.

Cryo-EM structures of a MexB–MexY chimeric efflux pump reveal that large open clefts are intrinsic to the MexY porter domain

In this study, cryo-EM structures of the chimeric RND transporter MexBYB, composed of MexB and MexY, are presented, revealing distinct symmetric-like and asymmetric conformations under apo and drug-supplemented conditions. These structures show that the unusually large open clefts observed in MexBYB, similar to those in MexY, represent a unique conformational feature of the MexY porter domain.

Crystal structure of endo-β-N-acetylglucosaminidase HSα

Structural analysis of the GH85 enzyme endo-β-N-acetylglucosaminidase HSα reveals a novel domain that is potentially involved in complex N-glycan recognition.