Crystal structure of the cytosolic Nudix-like domain of CD-NTase-associated protein 16 from Enterococcus faecalis

The cytosolic Nudix-like domain of the CBASS effector EfCap16 from E. faecalis was expressed, purified and crystallized, and its structure was determined by X-ray crystallography, revealing a canonical Nudix fold.

Crystal structure of the type VI secretion system adaptor protein Tla3 from Pseudomonas aeruginosa

The 1.8 Å resolution crystal structure of the adaptor protein Tla3 from the P. aeruginosa type VI secretion system is reported. The structure reveals a thiolase-like fold.

Crystal structure of human Dickkopf 4 cysteine-rich domain 1 and evaluation of conformational rigidity

This article describes the structural characterization of cysteine-rich domain 1 of Dickkopf 4. Comparison with the solution NMR structure indicates that regions displaying increased conformational variability may be relevant for protein–protein interactions.

Helical reconstruction of amyloids in cryoSPARC

We present practical guidelines for helical reconstruction of amyloid filaments in cryoSPARC, benchmarked against publicly available datasets. Our analysis defines current capabilities and limitations, and outlines optimization strategies to enable high-throughput structural studies and therapeutic discovery.