Crystal structure of the cytosolic Nudix-like domain of CD-NTase-associated protein 16 from Enterococcus faecalis

The cytosolic Nudix-like domain of the CBASS effector EfCap16 from E. faecalis was expressed, purified and crystallized, and its structure was determined by X-ray crystallography, revealing a canonical Nudix fold.

Helical reconstruction of amyloids in cryoSPARC

We present practical guidelines for helical reconstruction of amyloid filaments in cryoSPARC, benchmarked against publicly available datasets. Our analysis defines current capabilities and limitations, and outlines optimization strategies to enable high-throughput structural studies and therapeutic discovery.