Structure of recombinant Thermoascus aurantiacus xylanase TaXyn10 with an authentic N-terminal pyroglutamate

The high-resolution structure of the T. aurantiacus xylanase TaXyn10 expressed in Escherichia coli shows that recombinant production combined with split-intein-based purification recovers the native fold of TaXyn10 and permits the formation of an N-terminal pyroglutamate. These results demonstrate that this approach can enable recombinant proteins to adopt their native mature state in E. coli, including physiologically relevant post-translational modifications such as N-terminal pyroglutamate formation.

Structural insights into a putative BLF1-like deamidase from Methylomonas sp. Kb3

The crystal structure of a putative BLF1-like deamidase from Methylomonas sp. Kb3 reveals conservation of the catalytic cysteine–histidine dyad despite extensive sequence variation. These findings expand the diversity of BLF1-like deamidases and provide a structural framework for understanding their evolution and potential functions.

Crystal structures of the sweet-tasting protein honey truffle active component from Mattirolomyces terfezioides

Crystal structures of the sweet-tasting protein honey truffle active component are reported at resolutions ranging from 1.23 to 1.70 Å. The structures correlate well with a previously reported NMR solution-phase protein structure and reveal side-chain configurations that are critical in binding of the protein to the human sweet taste receptor and are also involved in ligand interactions in solution and self-association.

Serendipitous crystal structure of mJuniper, a rationally designed cyan fluorescent protein

We report the crystal structure of mJuniper, a rapidly maturing cyan fluorescent protein.

Sulfate-compatible density marks an intratrimeric junction in the Escherichia coli di­hydrolipo­amide succinyltransferase catalytic domain

The crystal structure of the catalytic domain of E. coli dihydrolipoamide succinyltransferase in space group F432 identifies a sulfate-compatible intratrimeric junction associated with Lys196 and α1-helix positioning near the active-site pocket.